Ppef1 (NM_001034935) Rat Untagged Clone

CAT#: RN215546

Ppef1 (untagged ORF) - Rat protein phosphatase, EF-hand calcium binding domain 1 (Ppef1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Ppef1
• Synonyms: MGC125115
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN215546 representing NM_001034935
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTGTGGCAGTTCTTCAAAAAAAGGCAAGAAATCTGAGAAAGTGGTGAGAGCTGCATTAATCATCC
  AAAATTGGTACCGACGGTACAGAGCTCGACTGAGCGCCAGACAACATTATGCCCTCGCCATCTTCCAGTC
  TATCGAATATGCTGACGAGCAAGGCCAGATGCAGCTATCCAGCTTCTTTTCTTTCATGTTGGAAAACTAT
  ACAAACACACACAAGGAAGATTCAGCATTAGTAAGTCGACTTTTTGAAAACACCAGGCTCGAAAGCAAGG
  ACAGAGAGGAATACGTGGGATTGATAGATGTGCCTGACTCCTATGACGGTCCTCGTCTGCAGTTTCCTCT
  CACGTTTACTGACATCAATTTACTTCTTCAGGCCTTCAAGCAACAGCAGACACTTCATGCTCACTATGTC
  TTAGAGGTGCTATTTGAAGCCAGGAAAATCCTGAAGCAGATGCCGAATTTCACTCGCATACAAACCTTTC
  CCGCCAAAGAAATAACAATCTGTGGTGATTTGCATGGGAAACTGGATGATCTTATGTTAATTTTCTATAA
  GAATGGCCTGCCTTCAGAGAAGAATCCATATGTGTTTAATGGTGATTTTGTAGATCGAGGAAACAATTCC
  ATGGAGATCCTAATGATCCTGCTTGTTAGTTTTCTTGTCTACCCCACTGACCTGCACCTCAATAGAGGGA
  ACCATGAAGACTTTATGATGAACCTGAGGTATGGCTTCACAAAAGAAATCTTACAGAAATACAAGCTACA
  TGGGAAAAAAATCTTACAAGTCTTGGAAGAACTCTATACCTGGCTCCCAATTGGTACAATTATTGACAAT
  GAAATCCTGGTCATACATGGTGGGATATCAGAATCCACGGACTTGAATATACTCCAGCAACTACAGAGAA
  ATAAAATGAAGTCTGTGTTAATGCCACCAATGTCAACAAATCAGGAATGTAACATAAAGAAAAATAAAGC
  AGGGCCGAGCGAACAGTCTGCTTCTGAACAGTTGACAAAGCTGGAATGGGAACAGATTATTGACCTTCTG
  TGGAGTGACCCCAGAGGCAAAAAAGGCTGTTATCCAAACACAAGCCGAGGAGGCGGCTGCTATTTTGGAC
  CAGATGTTACCTCTAAGGTTCTTAATAAAAACCAGTTGAAGATGGTCATTCGGTCTCATGAGTGTAAACC
  TGATGGGTATGAAATCTGTCATGATGGAAAGGTCATTACTGTATTTTCTGCTTCTAATTATTATGAAGAA
  GGCAGCAACCGAGGAGCTTACATCAGACTAAGTTATGGTACATCTCCACAATTTTTCCAGTACCAAGTGA
  CTAGTACATCATGCCTGAACCCTCTTTACCAAAGGGTGAATGCTATGGAATTCAGTGCCTTCAGGATACT
  AAAGGAAAGAATGATTGCAAGAAAAACTGACCTTATCAATGCTTTTGAGCTCCGAGATCACAGTAGGACA
  GGAAAGATTTCATTGGCCCAGTGGGCTTTTTCTATGGAGAGCATTTTGGGGCTGAACTTACCGTGGAGAT
  CTCTGAGTTCGCATCTGGTAAGCACTGACTCCAGCGGAAGCGTCGACTACATGTCCAGCTTTGATGACAT
  CCACATTGAGAAGCCGATGAAAGATATGAAGTCTGATCTCATTGAAACTATGTACAGATACCGATCTGAC
  CTGAAAATCATATTTAATATCATCGACACCGATCAGTCAGGTCTGATCTCCATGGATGAATTCCGGACCA
  TGTGGAAACTTTTCAATGCTCATTACAAAGTTCATATTGATGATTCCCAAATTGATGAGCTTGCCAGCAC
  AATGGACTCCAACAAAGATGGCAACATAGACTTTAACGAGTTTTTAAGGGCTTTCTATGTAGTCCATAAA
  TACGAGACGCCAGAGAGCCCTTTGAACAAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001034935
• Insert Size: 1923 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001034935.1, NP_001030107.1
• RefSeq Size: 2497 bp
• RefSeq ORF: 1923 bp
• Locus ID: 317498
• Cytogenetics: Xq14