Hfe (NM_001173435) Rat Untagged Clone

CAT#: RN215952

Hfe (untagged) - Rat hemochromatosis (Hfe), transcript variant 3

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Hfe
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN215952 representing NM_001173435
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCGATCAGCTGGGCTCCCTGTGCGGCTGCTATTGCTGCTGCTGTTGTTGCTGCTGTGGTCCGTGG
  CCCCGCAGGCGCTGCGGCCCGTGCCTACTTTGGTGAAAGTGACTCGCCACTGGGCCTCTACAGGGACCTC
  TCTAAGGTGTCAGGCTCTGAATTTCTTCCCCCAGAACATCACTATGAGGTGGTTGAAGGACAGCCAGCCC
  CTAGATGCCAAGGATGTCAACCCTGAGAACGTGCTGCCAAATGGGGATGGGACCTATCAGGGCTGGCTGA
  CCTTGGCTGTGGCCCCTGGAGAAGAGACAAGGTTCAGCTGTCAAGTGGAGCACCCAGGCCTGGATCAGCC
  TCTCACTGCCACTTGGGAGCCCTCACGGTCTCAGGACATGATTATTGGAATCATAAGTGGGATCACCATT
  TGTGCCATCTTCTTTGTTGGAATTCTGATCCTAGTCTTAAGGAAAAGGAAGGTTTCAGGAGGAACCATGG
  GTGACTATGTCTTAACAGAGTGTGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001173435
• ORF Size: 519 bp
• Insert Size: 519
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001173435.1, NP_001166906.1
• RefSeq Size: 1106
• RefSeq ORF: 519
• Locus ID: 29199
• Gene Summary: membrane protein; may be involved with iron metabolism; defects in human homolog lead to hereditary hemochromotosis, an iron storage disorder [RGD, Feb 2006]
  Transcript Variant: This variant (3) lacks two alternate in-frame exons compared to variant 1. The resulting protein (isoform 3) is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.