Setmar (NM_001025048) Rat Untagged Clone

CAT#: RN216334

Setmar (untagged) - Rat SET domain and mariner transposase fusion gene (Setmar)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Setmar
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN216334 representing NM_001025048
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGCGGAAGCTGTGAAAAAGCTTTCTTTCGAGATAGCACCATCTGAGGAGGAATCTGAGGCCACGA
  TAGAACAACAAGATGTAGCGTGCGGCCTGGAGAACTTGCCCGTGAGCCTGTGGCCCTTGGGGGCGGGGCC
  CAGGCCTAAACCTTTCCAGTATACTCCTGATCATGTAGCTGGGCCTGGAGTAGACATGGACCCCACACAA
  ATAACCTTCCCTGGTTGTGCTTGCATCAAAACTCCATGTGTCCCTGGTACTTGCTCCTGTCTTCGTCATG
  AGAGTAACTACAATGACAATTTATGCCTTAGGGATGTGGGATCAGAAGCAAAGTACGCCAAGCCAGTCTT
  TGAATGCAATGTTCTGTGCCAGTGTGGCGAGCACTGCAGAAACAGGGTGGTCCAGAGTGGTCTCCAGTTC
  CTTCTCCAGGTATTCCAGACAGAAAAAAAAGGCTGGGGACTTCGGACCTTGGAATATATACCCAAGGGAA
  GATTCGTCTGTGAGTATGCTGGGGAGGTATTAGGATTCTCTGAAGTACAAAGAAGAATTCACCTACAAAC
  AGCACATGACCCAAATTACATCATCGCCCTCAGGGAGCACACATACAATGGCCAGGTCATGGAAACATTT
  GTCGACCCTACCTACATAGGAAATATTGGGAGATTCCTCAATCATTCTTGTGAACCAAATCTGTTGATGA
  TTCCTGTCCGAATTGACTCAATGGTGCCCAAGCTCGCACTTTTTGCAGCCAAAGATATTTTGCCAGGAGA
  AGAACTCTCTTACGACTATTCAGGAAGATTCCTTAATCAAATAAGCAGTAAAGACAAAGAAAGGATAGAC
  TGTGGACAACCAAGGAAGCCTTGTTACTGCGGTGCCCAATCATGTGCCACTTTCCTGCCCTATGACAGTT
  CACTATATGGCCCCTTAGAAAATCCAGGCACCAGTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001025048
• Insert Size: 948 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025048.1, NP_001020219.1
• RefSeq Size: 1569 bp
• RefSeq ORF: 948 bp
• Locus ID: 500281
• Cytogenetics: 4q41
• Gene Summary: This gene encodes a histone-lysine N-methyltransferase that may be involved in the methylation of histone H3. In anthropoid primates this gene is a fusion gene of a SET histone-lysine N-methyltransferase and a mariner (MAR) family transposase. In all other species this gene contains only the SET domain. [provided by RefSeq, Feb 2013]