Pfkfb1 (NM_001271063) Rat Untagged Clone

CAT#: RN216743

Pfkfb1 (untagged) - Rat 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), transcript variant 2

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Pfkfb1
• Synonyms: Pfkfb01; PFRX
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN216743 representing NM_001271063
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCACCCACCGGACCTGCTTTGGGGGTCTGTAAATGCAAGAGAGCCATTGGAAAATTAGCGATGGAAG
  AAAAAGCCTCTAAGAGAACAGCCTCCATACCACAGTTCACTAATTCTCCCACGATGGTGATCATGGTGGG
  TTTACCAGCTCGAGGCAAGACCTACATCTCTACGAAGCTCACACGCTATCTCAACTGGATAGGAACACCA
  ACTAAAGTGTTTAATTTAGGTCAGTATCGACGAGAGGCAGTGAGTTACAGGAACTATGAATTCTTTCGCC
  CAGACAACACAGAGGCCCAGCTTATCAGGAAGCAGTGTGCTCTAGCAGCCCTAAAGGATGTCCATAAGTA
  TCTCAGCCGCGAGGAAGGTCATGTTGCGGTTTTTGATGCCACCAACACTACCAGAGAACGACGATCGTTG
  ATTCTACAGTTTGCTAAGGAACATGGTTATAAGGTCTTCTTTATTGAGTCTATTTGTAATGACCCCGAAA
  TCATTGCAGAAAACATCAAGCAAGTGAAACTTGGTAGTCCTGATTACATAGACTGTGACCAAGAAAAGGT
  TTTGGAAGACTTTCTAAAGAGAATAGAGTGCTATGAGATCAACTACCAACCTTTGGATGAGGAATTGGAC
  AGCCACCTGTCCTACATCAAGATCTTCGACGTGGGCACACGCTACATGGTAAATCGAGTGCAGGACCACG
  TTCAGAGCCGTACAGCCTACTACCTCATGAACATCCATGTCACACCTCGATCTATCTACCTATGCCGCCA
  TGGTGAGAGTGAACTCAACCTTAGAGGCCGCATTGGAGGTGACTCTGGCCTCTCAGCTCGGGGCAAGCAG
  TATGCCTATGCACTAGCCAACTTCATCCGGTCTCAAGGCATCAGCTCCCTGAAAGTATGGACTAGCCACA
  TGAAGAGGACCATTCAGACCGCTGAAGCCCTAGGTGTCCCCTATGAACAGTGGAAGGCCCTGAATGAGAT
  TGATGCGGGTGTCTGTGAAGAGATGACCTATGAAGAAATTCAGGAACACTACCCTGAGGAATTTGCACTA
  CGGGACCAGGATAAATATCGTTACCGCTATCCCAAGGGAGAGTCCTATGAGGATCTGGTTCAGCGTCTTG
  AACCAGTTATAATGGAGCTAGAACGGCAAGAAAATGTACTGGTGATCTGTCACCAGGCTGTCATGCGGTG
  CCTCCTGGCATACTTCCTGGATAAAAGTTCAGATGAGCTGCCCTATCTCAAGTGTCCTCTGCATACTGTG
  CTCAAACTCACACCTGTGGCTTATGGCTGCAGAGTGGAGTCCATCTACCTGAATGTGGAGGCTGTGAACA
  CACACCGGGACAAGCCTGAGAATGTGGACATCACCCGTGAAGCTGAGGAAGCCTTGGACACTGTACCTGC
  CCATTACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001271063
• ORF Size: 1410 bp
• Insert Size: 1410
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001271063.1, NP_001257992.1
• RefSeq Size: 1707
• RefSeq ORF: 1410
• Locus ID: 24638
• Gene Summary: bifunctional enzyme; catalyzes synthesis and degradation of fructose 2,6-bisphosphate, a ubiquitous stimulator of glycolysis [RGD, Feb 2006]
  Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.