Ddx19b (NM_001005895) Rat Untagged Clone

CAT#: RN216761

Ddx19b (untagged) - Rat DEAD (Asp-Glu-Ala-As) box polypeptide 19B (Ddx19b)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Ddx19b
• Synonyms: Zd10a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN216761 representing NM_001005895
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACGGATTCTTGGGCCCTAGCAGTGGACGAGCAGGAAGCCGCCGCTGAGTCGTTGAGCAACTTGC
  ATCTTAAAGAGGAGAAAATCAAACCAGATGCCAATGGTGCTGTTGTTAAGACCAGTGCTAACACAGAGAA
  GACAGAGGAAGATGAGAAAGAGGACAGAGCTGCTCAGTCTTTACTCAACAAGCTGATCAGAAGCAATCTT
  GTGGATAACACCAACCAAGTGGAAGTCCTGCAGAGGGACCCGAGCTCCCCGCTCTACTCAGTGAAGTCCT
  TCGAGGAGCTTCGCCTGAAACCACAGCTTCTCCAGGGAGTCTATGCCATGGGTTTCAACCGCCCTTCCAA
  GATCCAGGAGAATGCACTGCCTTTGATGCTTGCTGAGCCCCCACAGAACTTAATTGCCCAGTCTCAGTCT
  GGTACTGGGAAAACAGCTGCCTTCGTGTTGGCCATGCTCAGCCAAGTAGAGCCTGCAAACAACCATCCCC
  AGTGTCTCTGCCTCTCTCCAACCTATGAGCTGGCTCTTCAAACAGGAAAAGTGATTGAGCAGATGGGCAA
  ATTTTACCCTGAACTGAAGTTAGCTTATGCTGTTCGAGGCAATAAATTGGAAAGAGGTCAGAAGGTCGGT
  GAGCAGATTGTCATCGGTACCCCTGGGACCGTCCTGGACTGGTGCTCCAAACTGAAGTTCATTGACCCCA
  AGAAGATCAAGGTGTTTGTTCTGGACGAGGCTGATGTGATGATAGCGACTCAGGGCCACCAAGACCAGAG
  CATCCGCATCCAGAGGATGCTGCCCAGAAACTGCCAGATGCTGCTTTTCTCTGCCACCTTTGAAGACTCA
  GTGTGGAAGTTTGCCCAGAAGGTGGTCCCAGACCCCAACATCATCAAGCTGAAGCGTGAAGAGGAGACGT
  TGGACACCATCAAACAGTATTATGTCCTTTGCAATAACAGAGAGGAGAAGTTCCAGGCCCTGTGCAACTT
  GTACGGGGCCATCACCATCGCCCAAGCCATGATCTTCTGCCATACCCGCAAAACAGCTAGCTGGCTGGCG
  GCAGAGCTCTCCAAAGAGGGCCACCAGGTGGCCCTGCTGAGTGGAGAGATGATGGTGGAGCAGAGGGCTG
  CTGTGATCGAGCGCTTCAGAGAAGGCAAAGAGAAGGTTCTGGTGACCACCAACGTGTGTGCCCGTGGTAT
  TGATGTTGAACAAGTGTCTGTGGTCATCAATTTTGACCTTCCTGTGGACAAGGACGGGAACCCAGACAAT
  GAGACCTACCTGCACCGCATTGGGCGCACAGGCCGCTTTGGCAAGAGGGGCCTGGCAGTAAACATGGTTG
  ACAGCAAGCACAGCATGAATATCCTCAACAGAATCCAGGAGCATTTCAATAAGAAGATAGAAAGATTGGA
  CACAGATGACTTGGACGAGATCGAGAAAATAGCCAACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001005895
• Insert Size: 1440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005895.2, NP_001005895.2
• RefSeq Size: 6482 bp
• RefSeq ORF: 1440 bp
• Locus ID: 690693
• Cytogenetics: 19q12
• Gene Summary: plays a role in zinc induced apoptosis [RGD, Feb 2006]