Gria2 (NM_001083811) Rat Untagged Clone

CAT#: RN217297

Gria2 (untagged) - Rat glutamate receptor, ionotropic, AMPA 2 (Gria2), transcript variant 2

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Gria2
• Synonyms: GluA2; gluR-B; GluR-K2; GluR2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN217297 representing NM_001083811
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAAAGATTATGCATATTTCTGTCCTCCTTTCTCCTGTTTTATGGGGACTGATTTTTGGTGTCTCTT
  CTAACAGCATACAGATAGGGGGGCTATTTCCAAGGGGCGCTGATCAAGAATACAGTGCATTTCGGGTAGG
  GATGGTTCAGTTTTCCACTTCGGAGTTCAGACTGACACCCCATATCGACAATTTGGAGGTAGCCAACAGT
  TTCGCAGTCACCAATGCTTTCTGCTCCCAGTTTTCAAGAGGAGTCTACGCAATTTTTGGATTTTATGACA
  AGAAGTCTGTAAATACCATCACATCATTCTGTGGGACACTCCATGTGTCCTTCATCACACCTAGCTTCCC
  AACAGATGGCACACATCCATTTGTCATCCAGATGCGACCTGACCTCAAAGGAGCACTCCTTAGCTTGATT
  GAGTACTACCAATGGGACAAGTTCGCATACCTCTATGACAGTGACAGAGGCTTATCAACACTGCAAGCTG
  TTCTGGATTCTGCTGCAGAGAAGAAGTGGCAGGTGACTGCTATCAATGTGGGGAACATCAACAATGACAA
  GAAAGATGAGACCTACAGATCGCTCTTTCAAGATCTGGAGTTAAAAAAAGAACGGCGTGTAATCCTGGAC
  TGTGAAAGGGATAAAGTAAATGACATTGTGGACCAGGTTATTACCATTGGAAAACATGTTAAAGGGTACC
  ATTATATCATTGCAAATCTGGGATTCACTGATGGGGACCTGCTGAAAATTCAGTTTGGAGGAGCAAATGT
  CTCTGGATTTCAGATTGTAGACTACGACGATTCCCTGGTGTCTAAATTTATAGAAAGATGGTCAACACTG
  GAAGAGAAAGAATACCCTGGAGCACACACAGCGACAATTAAGTATACTTCGGCCCTGACGTATGATGCTG
  TCCAAGTGATGACTGAAGCATTCCGTAACCTTCGGAAGCAGAGGATTGAAATATCCCGGAGAGGAAATGC
  AGGAGATTGTTTGGCCAACCCAGCTGTGCCCTGGGGACAAGGGGTCGAAATAGAAAGGGCCCTCAAGCAG
  GTTCAAGTTGAAGGCCTCTCTGGAAATATAAAGTTTGACCAGAATGGAAAACGAATAAACTACACAATTA
  ACATCATGGAGCTCAAAACAAATGGACCCCGGAAGATTGGGTACTGGAGTGAAGTGGATAAAATGGTTGT
  CACCCTAACTGAGCTCCCATCAGGAAATGACACGTCTGGGCTTGAAAACAAGACTGTGGTGGTCACCACA
  ATATTGGAATCTCCATATGTTATGATGAAGAAAAATCATGAAATGCTTGAAGGGAATGAGCGTTACGAGG
  GCTACTGTGTTGACTTAGCTGCAGAAATTGCTAAACACTGTGGGTTCAAGTACAAGCTGACTATTGTTGG
  GGATGGCAAGTATGGGGCCAGGGATGCCGACACCAAAATTTGGAATGGTATGGTTGGAGAGCTTGTCTAC
  GGGAAAGCTGACATTGCAATTGCTCCATTAACTATCACTCTCGTGAGAGAAGAGGTGATTGACTTCTCCA
  AGCCCTTCATGAGTCTTGGAATCTCTATCATGATCAAGAAGCCTCAGAAGTCCAAACCAGGAGTGTTTTC
  CTTTCTTGATCCTTTAGCCTATGAGATCTGGATGTGCATTGTGTTTGCCTACATTGGGGTCAGTGTAGTT
  TTATTCCTGGTCAGCAGATTTAGCCCCTACGAGTGGCACACTGAGGAATTTGAAGATGGAAGAGAAACAC
  AAAGTAGTGAATCAACTAATGAATTTGGGATTTTTAATAGTCTCTGGTTTTCCTTGGGTGCCTTTATGCG
  GCAAGGATGCGATATTTCGCCAAGATCCCTCTCTGGGCGCATTGTTGGAGGTGTGTGGTGGTTCTTTACC
  CTGATCATAATCTCCTCCTACACGGCTAACTTAGCTGCCTTCCTGACTGTAGAGAGGATGGTGTCTCCCA
  TCGAAAGTGCTGAGGATCTGTCTAAGCAAACAGAAATTGCTTATGGAACATTAGACTCTGGCTCCACTAA
  AGAGTTTTTCAGGAGATCTAAAATCGCAGTGTTTGATAAAATGTGGACTTATATGAGGAGTGCAGAGCCC
  TCTGTGTTTGTGAGGACTACCGCAGAAGGAGTAGCCAGAGTCCGGAAATCCAAAGGAAAGTATGCCTACT
  TGCTGGAGTCCACAATGAACGAGTACATCGAGCAGAGGAAGCCTTGTGACACCATGAAAGTGGGAGGAAA
  CTTGGATTCCAAAGGCTACGGCATCGCCACACCTAAAGGATCCTCATTAGGAAATGCGGTTAACCTCGCA
  GTACTAAAACTGAATGAACAAGGCCTGTTGGACAAATTGAAAAACAAATGGTGGTACGACAAAGGAGAGT
  GCGGCAGCGGGGGAGGTGATTCCAAGGAAAAGACCAGTGCCCTCAGTCTGAGCAACGTTGCTGGAGTATT
  CTACATCCTTGTCGGGGGCCTTGGTTTGGCAATGCTGGTGGCTTTGATTGAGTTCTGTTACAAGTCAAGG
  GCCGAGGCGAAACGAATGAAGGTGGCAAAGAATCCACAGAATATTAACCCATCTTCCTCGCAGAATTCCC
  AGAATTTTGCAACTTATAAGGAAGGTTACAACGTATATGGCATCGAGAGTGTTAAAATTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001083811
• ORF Size: 2652 bp
• Insert Size: 2652
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001083811.1, NP_001077280.1
• RefSeq Size: 6745
• RefSeq ORF: 2652
• Locus ID: 29627
• Gene Summary: Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, Gria1-4. The subunit encoded by this gene (Gria2) is subject to RNA editing (Q/R and R/G), which is thought to render the channels impermeable to Ca(2+), and to affect the kinetic aspects of these channels in rat brain. Alternative splicing, resulting in transcript variants encoding different isoforms (flip and flop), has been noted for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) uses an alternate exon in the 3' coding region compared to transcript variant 1, and encodes an isoform (2, also known as the flop isoform) that is the same length as isoform 1, but with few amino acid differences. RNA editing changes Gln607Arg (CAG->CGG) and Arg764Gly (AGA->GGA). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.