Nars (NM_001025635) Rat Tagged ORF Clone

CAT#: RR200129

Nars (Myc-DDK-tagged ORF) - Rat asparaginyl-tRNA synthetase (Nars), transcript variant 1, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nars
• Synonyms: LRRGT00113
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200129 representing NM_001025635
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTCAGAGGTGATCAGGGGGACTGCAGACATGGTTCTAGAGCTCTATGTCTCTGACCGAGAAGGAA
  ATGACGCAACGGGGGATGGAACCAAGGAGAAACCATTTAAAACAGGCCTAAAGGCTTTGATGACAGTTGG
  AAAAGAGCCGTTTCCCACCATTTATGTGGATTCACAGAAGGAAAATGAGAGGTGGGACGTTATTTCTAAG
  TCACAGATGAAGAACATCAAAAAGATGTGGCACCGAGAACAGATGAAGAGTGATTCCCGGGAGAAGAAAG
  AGGCAGAAGATAACTTACGAAGAGAAAAGAACCTGGAGGAAGCAAAAAAAATTATCATTAAAAACGACCC
  GAGCCTGCCGGAGCCAGCATGTGTAAAGATTAGTGCATTAGAAGGATACAGAGGCCAGAGAGTGAAGGTG
  TTTGGCTGGGTCCACAGGTTACGAAGGCAAGGAAAGAATCTGATGTTTTTGGTGTTGCGAGATGGTACGG
  GTTATCTTCAGTGTGTCTTGTCAGATGACTTGTGTCAGTGTTACAATGGAGTAGTCTTGTCCACTGAGAG
  TAGCGTGGCGGTATACGGGACACTGAACCTAACTCCAAAGGGCAAACAGGCTCCGGGAGGCCATGAGCTG
  AGCTGTGACTTCTGGGAACTGGTGGGGCTGGCCCCAGCTGGAGGAGCTGACAACCTGATCAATGAGGAGT
  CTGATGTGGACGTCCAGCTCAACAACCGGCATATGATGATCCGGGGAGAGAACATGTCCAAAATCCTGAA
  AGCACGGTCCATGATCACCAGGTGCTTTAGGGACCACTTCTTCGACAGAGGCTACTGTGAAGTAACCACT
  CCAACACTGGTGCAGACACAGGTGGAAGGTGGGGCCACACTCTTCAAGCTTGACTATTTTGGGGAAGAAG
  CATTTTTGACCCAGTCCTCACAGCTGTACCTGGAGACCTGCCTTCCAGCCCTGGGAGATGTTTTTTGCAT
  AGCCCAGTCTTACAGGGCTGAACAGTCCAGGACACGAAGGCATCTGGCTGAGTTCACTCACGTGGAAGCC
  GAGTGTCCTTTCCTGACCTTCGAGGACCTCCTGAGCCGTCTAGAGGACCTGGTGTGTGACGTGGTGGACA
  GAGTCTTGAAGTCACCAGTGGCGAGCATAGTGTATGACCTTAACCCGAACTTTAAAGCCCCCAAACGGCC
  TTTCCGGCGGATGAACTATTCAGATGCCATTGAGTGGCTGAGGGAGCACGATGTAAAGAAAGAAGACGGG
  ACGTTCTACGAGTTCGGAGACGATATTCCCGAAGCGCCTGAGAGACTGATGACAGACACCATTAATGAAC
  CAATCCTGCTGTGTCGGTTTCCTGTGGAGATCAAGTCCTTCTACATGCAGCGCTGTCCTGAGGATCCTCG
  GCTTACCGAATCTGTGGATGTGTTGATGCCCAATGTCGGTGAGATTGTGGGAGGCTCAATGCGCTCCTGG
  GACAGTGAGGAGATTCTAGAAGGCTATAAAAGGGAAGGGATTGACCCCGCTCCTTATTACTGGTATACAG
  ATCAGAGAAAATATGGTACATGTCCTCACGGAGGGTATGGCTTGGGCTTGGAACGATTTCTAAGCTGGAT
  TCTGAACAGGTATCACATCCGAGACGTGTGCTTGTACCCGAGATTTCTCCAGCGCTGCAGGCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200129 representing NM_001025635
  Red=Cloning site Green=Tags(s)
  
  MSSEVIRGTADMVLELYVSDREGNDATGDGTKEKPFKTGLKALMTVGKEPFPTIYVDSQKENERWDVISK
  SQMKNIKKMWHREQMKSDSREKKEAEDNLRREKNLEEAKKIIIKNDPSLPEPACVKISALEGYRGQRVKV
  FGWVHRLRRQGKNLMFLVLRDGTGYLQCVLSDDLCQCYNGVVLSTESSVAVYGTLNLTPKGKQAPGGHEL
  SCDFWELVGLAPAGGADNLINEESDVDVQLNNRHMMIRGENMSKILKARSMITRCFRDHFFDRGYCEVTT
  PTLVQTQVEGGATLFKLDYFGEEAFLTQSSQLYLETCLPALGDVFCIAQSYRAEQSRTRRHLAEFTHVEA
  ECPFLTFEDLLSRLEDLVCDVVDRVLKSPVASIVYDLNPNFKAPKRPFRRMNYSDAIEWLREHDVKKEDG
  TFYEFGDDIPEAPERLMTDTINEPILLCRFPVEIKSFYMQRCPEDPRLTESVDVLMPNVGEIVGGSMRSW
  DSEEILEGYKREGIDPAPYYWYTDQRKYGTCPHGGYGLGLERFLSWILNRYHIRDVCLYPRFLQRCRP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025635
• ORF Size: 1674 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025635.2, NP_001020806.2
• RefSeq Size: 2640 bp
• RefSeq ORF: 1677 bp
• Locus ID: 291556
• Cytogenetics: 18q12.1
• MW: 64.1 kDa