F10 (NM_017143) Rat Tagged ORF Clone

CAT#: RR205892

F10 (Myc-DDK-tagged ORF) - Rat coagulation factor X (F10), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: F10
• Synonyms: MGC108722
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205892 representing NM_017143
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGCCCGGTGCGACTCAGCCTTCTCTATGTTGTCCTGGCCAGCCTCCTGCTCCCGGGGAGAAGTG
  TGTTTATTAACCGGGAACGAGCCAACAACGTCCTGCAGAGGATTCGGAGAGCAAACTCATTTTTTGAGGA
  GATAAAGAAAGGAAATCTGGAAAGGGAGTGTGTGGAAGAAATTTGTTCTTTTGAAGAGGCCCGCGAAGTC
  TTTGAGGACAATGAGAAGACGACAGAATTCTGGAACAAATATGAAGATGGTGACCAGTGTGAGAGCAGTC
  CTTGCCAGAACCAAGGCGAGTGTCGAGATGGCCTTGGGAGTTATACATGCACCTGCACGGAGGGATTCGA
  AGGCAAAAACTGTGAACTCTTTGTTCGGAAACTCTGCAGCCTAGACAACGGAGACTGCGACCAGTTCTGC
  CGTGAGGAGCAGAACTCAGTGGTGTGCTCCTGTGCCAAGGGTTACTTCCTGGGTAATGACGGCAAGTCCT
  GCCTCTCCACAGCTCCCTTCCCCTGTGGAAAAACCAATAAAGGACGCGCGAAGAGGTCTGTGGCCCTAAA
  TACCAGCAACAGTGAACCTGACCCTGAAGACCTCATGCCTGATGCAGATATTCTATATCCTACTGAGAGT
  CCTTCTGAACTGCTCAATCTCAACAAGACAGAGCCTGAAGCAAACAGTGATGACGTTATTCGGATTGTGG
  GTGGCCAAGAATGCAAGAGAGGAGAATGTCCTTGGCAGGCTCTGCTCTTTAGCGACGAAGAGACTGATGG
  GTTCTGTGGGGGCACCATCTTGAATGAGTTCTACATCCTCACTGCCGCCCATTGTCTCCATCAAGCCAAG
  CGATTCAAGGTGAGGGTAGGGGACCTGAACACGGAACAGGAAGACGGCGGCGAGATGGTGCACGAGGTGG
  ATATGATCATCAAGCACAACAAGTTTCAGAGGGACACCTACGACTTCGACATTGCCATGCTGAGGCTGAA
  GACCCCCATCACGTTCCGCGAGAACGTGGCCCCTGCCTGCTTGCCTCAGAAAGACTGGGCCGAGGCCACA
  CTGATGACGCAAAAGACAGGCATCGTGAGCGGGTTTGGACGCACGCACGAGAAGGGCCGCCAGTCCAAAG
  TCCTGAAGATGATGGAGGTGCCCTATGTGGACCGCAACACCTGCAGGCTCTCCACCAGCTTCAGCATTAC
  GCAGAATATGTTCTGCGCAGGCTATGACGCCAAGCAAGAGGACGCCTGCCAGGGGGACAGCGGTGGCCCT
  CACGTCACACGATTCAAAGACACCTACTTTGTGACCGGCATCGTCAGCTGGGGAGAGGGGTGTGCAAGGA
  AAGGGAAATACGGCATCTACACGAAGGTCACGGCCTTCCTCAAATGGATTGACAGGTCCATGAAAGCCAG
  GGTGGGGCCCACCTCTGAGACCCCAAGGTTAACCCATCCACCCTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205892 representing NM_017143
  Red=Cloning site Green=Tags(s)
  
  MESPVRLSLLYVVLASLLLPGRSVFINRERANNVLQRIRRANSFFEEIKKGNLERECVEEICSFEEAREV
  FEDNEKTTEFWNKYEDGDQCESSPCQNQGECRDGLGSYTCTCTEGFEGKNCELFVRKLCSLDNGDCDQFC
  REEQNSVVCSCAKGYFLGNDGKSCLSTAPFPCGKTNKGRAKRSVALNTSNSEPDPEDLMPDADILYPTES
  PSELLNLNKTEPEANSDDVIRIVGGQECKRGECPWQALLFSDEETDGFCGGTILNEFYILTAAHCLHQAK
  RFKVRVGDLNTEQEDGGEMVHEVDMIIKHNKFQRDTYDFDIAMLRLKTPITFRENVAPACLPQKDWAEAT
  LMTQKTGIVSGFGRTHEKGRQSKVLKMMEVPYVDRNTCRLSTSFSITQNMFCAGYDAKQEDACQGDSGGP
  HVTRFKDTYFVTGIVSWGEGCARKGKYGIYTKVTAFLKWIDRSMKARVGPTSETPRLTHPPY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017143
• ORF Size: 1446 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017143.1, NM_017143.2, NP_058839.1
• RefSeq Size: 1618
• RefSeq ORF: 1449
• Locus ID: 29243
• MW: 54.3 kDa
• Gene Summary: clotting factor that competes with prothrombin in the ER membrane for binding to gamma carboxylase [RGD, Feb 2006]