Cldn4 (NM_001012022) Rat Tagged ORF Clone

CAT#: RR210685

  • TrueORF®

Cldn4 (Myc-DDK-tagged ORF) - Rat claudin 4 (Cldn4), (10 ug)


  "NM_001012022" in other vectors (3)

Reconstitution Protocol

USD 420.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Symbol Cldn4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR210685 representing NM_001012022
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTCCATGGGGCTGCAGGTGCTGGGAATCTCCTTGGCCGTCCTGGGCTGGTTAGGAGTCATCCTGA
GTTGTTCGCTCCCCATGTGGCGGGTGACCGCCTTCATCGGCAGCAACATCGTCACGGCGCAGACGAGCTG
GGAAGGCCTGTGGATGAACTGCGTGGTGCAGAGCACCGGCCAGATGCAGTGCAAGATGTACGACTCGATG
CTGGCCCTGCCGCAGGACCTGCAGGCCGCCCGAGCCCTGATGGTCATCAGCATCATCGTGGGTGCTCTGG
GGATGCTTCTCTCAGTCGTAGGGGGCAAGTGCACCAACTGCATGGAGGACGAGACCGTCAAGGCCAAGGT
CATGATCACAGCCGGAGCCGTGTTCATCGTGGCAAGCATGCTGATTATGGTGCCTGTGTCCTGGACGGCA
CACAACGTCATCCGCGACTTCTACAACCCTCTGGTGGCTTCCGGGCAGAAAAGGGAGATGGGGGCCTCGC
TTTACATCGGCTGGGCGGCTTCTGGGCTGCTGCTCCTGGGAGGAGGCCTCCTCTGCTGCAATTGCCCACC
TCGCCGCAACGAAAAGCCCTACTCCGCCAAGTACTCCGCCGCCCGCTCTGTTCCCGCCAGCAACTATGTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR210685 representing NM_001012022
Red=Cloning site Green=Tags(s)

MASMGLQVLGISLAVLGWLGVILSCSLPMWRVTAFIGSNIVTAQTSWEGLWMNCVVQSTGQMQCKMYDSM
LALPQDLQAARALMVISIIVGALGMLLSVVGGKCTNCMEDETVKAKVMITAGAVFIVASMLIMVPVSWTA
HNVIRDFYNPLVASGQKREMGASLYIGWAASGLLLLGGGLLCCNCPPRRNEKPYSAKYSAARSVPASNYV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001012022
ORF Size 630 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001012022.1, NP_001012022.1
RefSeq Size 1824 bp
RefSeq ORF 633 bp
Locus ID 304407
Cytogenetics 12q12
MW 22.9 kDa

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