P4hb (NM_012998) Rat Tagged ORF Clone

CAT#: RR213352

P4hb (Myc-DDK-tagged ORF) - Rat prolyl 4-hydroxylase, beta polypeptide (P4hb), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: P4hb
• Synonyms: PDI; PDIR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213352 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAGCCGTGCTTTGCTGTGCCTGGCCCTGGCCTGGGCGGCTAGGGTGGGCGCCGACGCTCTGGAGG
  AGGAGGACAACGTCCTGGTGCTGAAGAAGAGCAACTTCGCAGAGGCGCTGGCGGCGCACAACTACCTGCT
  GGTGGAGTTCTATGCCCCATGGTGTGGCCACTGCAAAGCACTGGCCCCAGAGTATGCCAAAGCTGCTGCA
  AAACTGAAGGCAGAAGGCTCTGAGATCCGACTAGCAAAGGTGGACGCCACAGAAGAGTCTGACCTGGCCC
  AGCAGTATGGTGTCCGTGGCTACCCCACAATCAAGTTCTTCAAGAATGGAGACACAGCCTCCCCAAAGGA
  ATATACAGCTGGCAGGGAAGCTGACGACATTGTGAACTGGCTGAAGAAACGCACAGGCCCAGCAGCCACA
  ACCCTGTCTGACACTGCAGCTGCAGAGTCCTTGGTGGACTCAAGCGAAGTGACGGTCATCGGCTTCTTCA
  AGGACGCAGGGTCAGACTCCGCCAAGCAGTTCTTGCTGGCAGCAGAGGCTGTTGATGACATACCTTTTGG
  AATCACTTCCAATAGCGATGTGTTTTCCAAGTACCAGCTGGACAAGGATGGGGTGGTCCTCTTTAAGAAG
  TTTGATGAAGGCCGCAACAATTTTGAAGGTGAGATCACCAAGGAGAAGCTGTTAGACTTCATCAAGCACA
  ACCAGCTGCCTTTGGTCATCGAGTTCACTGAACAGACAGCTCCAAAGATTTTCGGAGGTGAAATCAAAAC
  ACATATTCTGCTGTTCCTGCCCAAGAGTGTGTCTGACTACGATGGCAAATTGAGCAACTTTAAGAAAGCG
  GCCGAGGGCTTTAAGGGCAAGATCCTGTTCATCTTCATCGATAGTGACCACACTGACAACCAGCGCATAC
  TTGAGTTCTTTGGCCTGAAGAAGGAGGAATGTCCAGCTGTGCGGCTTATTACCCTGGAGGAAGAGATGAC
  CAAGTACAAACCGGAGTCAGACGAGCTGACAGCTGAGAAGATCACACAATTTTGCCACCACTTCCTGGAG
  GGCAAGATCAAGCCCCACCTGATGAGCCAGGAACTGCCTGAAGACTGGGACAAGCAGCCAGTGAAAGTGC
  TAGTTGGGAAAAACTTTGAGGAGGTTGCTTTTGATGAGAAAAAGAACGTGTTTGTTGAATTCTATGCTCC
  CTGGTGTGGTCACTGCAAGCAGCTAGCCCCGATTTGGGATAAACTGGGAGAGACATACAAAGACCATGAG
  AATATCGTCATCGCTAAGATGGACTCAACAGCCAATGAGGTGGAAGCTGTGAAGGTGCACAGCTTTCCCA
  CACTCAAGTTCTTCCCAGCAAGTGCAGACAGAACGGTCATTGATTACAACGGTGAGCGGACACTAGATGG
  TTTTAAGAAATTCTTGGAGAGCGGTGGCCAGGATGGAGCGGGGGACAATGACGACCTCGACCTAGAAGAA
  GCTTTAGAGCCAGATATGGAAGAAGACGACGATCAGAAAGCCGTGAAGGATGAACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213352 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLSRALLCLALAWAARVGADALEEEDNVLVLKKSNFAEALAAHNYLLVEFYAPWCGHCKALAPEYAKAAA
  KLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFKNGDTASPKEYTAGREADDIVNWLKKRTGPAAT
  TLSDTAAAESLVDSSEVTVIGFFKDAGSDSAKQFLLAAEAVDDIPFGITSNSDVFSKYQLDKDGVVLFKK
  FDEGRNNFEGEITKEKLLDFIKHNQLPLVIEFTEQTAPKIFGGEIKTHILLFLPKSVSDYDGKLSNFKKA
  AEGFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESDELTAEKITQFCHHFLE
  GKIKPHLMSQELPEDWDKQPVKVLVGKNFEEVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHE
  NIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQDGAGDNDDLDLEE
  ALEPDMEEDDDQKAVKDEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012998
• ORF Size: 1527 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012998.1, NM_012998.2, NP_037130.2
• RefSeq Size: 2460
• RefSeq ORF: 1530
• Locus ID: 25506
• MW: 57 kDa
• Gene Summary: may be identical to protein disulfide isomerase, iodothyronine 5 monodeiodinase (5MD), and membrane associated thyroid hormone binding protein MTHBP. [RGD, Jun 2010]