PDE5A (NM_033437) Human Untagged Clone

CAT#: SC100003

PDE5A (untagged)-Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDE5A
• Synonyms: CGB-PDE; CN5A; PDE5
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_033437, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGTCAATGCATGGTTTGCTGAGAGAGTTCACACCATCCCTGTGTGCAAGGAAGGTATCAGAGGCCACA
  CCGAATCTTGCTCTTGTCCCTTGCAGCAGAGTCCTCGTGCAGATAACAGTGCCCCTGGAACACCAACCAG
  GAAAATCTCTGCCTCTGAATTTGACCGGCCTCTTAGACCCATTGTTGTCAAGGATTCTGAGGGAACTGTG
  AGCTTCCTCTCTGACTCAGAAAAGAAGGAACAGATGCCTCTAACCCCTCCAAGGTTTGATCATGATGAAG
  GGGACCAGTGCTCAAGACTCTTGGAATTAGTGAAGGATATTTCTAGTCATTTGGATGTCACAGCCTTATG
  TCACAAAATTTTCTTGCATATCCATGGACTGATATCTGCTGACCGCTATTCCCTGTTCCTTGTCTGTGAA
  GACAGCTCCAATGACAAGTTTCTTATCAGCCGCCTCTTTGATGTTGCTGAAGGTTCAACACTGGAAGAAG
  TTTCAAATAACTGTATCCGCTTAGAATGGAACAAAGGCATTGTGGGACATGTGGCAGCGCTTGGTGAGCC
  CTTGAACATCAAAGATGCATATGAGGATCCTCGGTTCAATGCAGAAGTTGACCAAATTACAGGCTACAAG
  ACACAAAGCATTCTTTGTATGCCAATTAAGAATCATAGGGAAGAGGTTGTTGGTGTAGCCCAGGCCATCA
  ACAAGAAATCAGGAAACGGTGGGACATTTACTGAAAAAGATGAAAAGGACTTTGCTGCTTATTTGGCATT
  TTGTGGTATTGTTCTTCATAATGCTCAGCTCTATGAGACTTCACTGCTGGAGAACAAGAGAAATCAGGTG
  CTGCTTGACCTTGCTAGTTTAATTTTTGAAGAACAACAATCATTAGAAGTAATTTTGAAGAAAATAGCTG
  CCACTATTATCTCTTTCATGCAAGTGCAGAAATGCACCATTTTCATAGTGGATGAAGATTGCTCCGATTC
  TTTTTCTAGTGTGTTTCACATGGAGTGTGAGGAATTAGAAAAATCATCTGATACATTAACAAGGGAACAT
  GATGCAAACAAAATCAATTACATGTATGCTCAGTATGTCAAAAATACTATGGAACCACTTAATATCCCAG
  ATGTCAGTAAGGATAAAAGATTTCCCTGGACAACTGAAAATACAGGAAATGTAAACCAGCAGTGCATTAG
  AAGTTTGCTTTGTACACCTATAAAAAATGGAAAGAAGAATAAAGTTATAGGGGTTTGCCAACTTGTTAAT
  AAGATGGAGGAGAATACTGGCAAGGTTAAGCCTTTCAACCGAAATGACGAACAGTTTCTGGAAGCTTTTG
  TCATCTTTTGTGGCTTGGGGATCCAGAACACGCAGATGTATGAAGCAGTGGAGAGAGCCATGGCCAAGCA
  AATGGTCACATTGGAGGTTCTGTCGTATCATGCTTCAGCAGCAGAGGAAGAAACAAGAGAGCTACAGTCG
  TTAGCGGCTGCTGTGGTGCCATCTGCCCAGACCCTTAAAATTACTGACTTTAGCTTCAGTGACTTTGAGC
  TGTCTGATCTGGAAACAGCACTGTGTACAATTCGGATGTTTACTGACCTCAACCTTGTGCAGAACTTCCA
  GATGAAACATGAGGTTCTTTGCAGATGGATTTTAAGTGTTAAGAAGAATTATCGGAAGAATGTTGCCTAT
  CATAATTGGAGACATGCCTTTAATACAGCTCAGTGCATGTTTGCTGCTCTAAAAGCAGGCAAAATTCAGA
  ACAAGCTGACTGACCTGGAGATACTTGCATTGCTGATTGCTGCACTAAGCCACGATTTGGATCACCGTGG
  TGTGAATAACTCTTACATACAGCGAAGTGAACATCCACTTGCCCAGCTTTACTGCCATTCAATCATGGAA
  CACCATCATTTTGACCAGTGCCTGATGATTCTTAATAGTCCAGGCAATCAGATTCTCAGTGGCCTCTCCA
  TTGAAGAATATAAGACCACGTTGAAAATAATCAAGCAAGCTATTTTAGCTACAGACCTAGCACTGTACAT
  TAAGAGGCGAGGAGAATTTTTTGAACTTATAAGAAAAAATCAATTCAATTTGGAAGATCCTCATCAAAAG
  GAGTTGTTTTTGGCAATGCTGATGACAGCTTGTGATCTTTCTGCAATTACAAAACCCTGGCCTATTCAAC
  AACGGATAGCAGAACTTGTAGCAACTGAATTTTTTGATCAAGGAGACAGAGAGAGAAAAGAACTCAACAT
  AGAACCCACTGATCTAATGAACAGGGAGAAGAAAAACAAAATCCCAAGTATGCAAGTTGGGTTCATAGAT
  GCCATCTGCTTGCAACTGTATGAGGCCCTGACCCACGTGTCAGAGGACTGTTTCCCTTTGCTAGATGGCT
  GCAGAAAGAACAGGCAGAAATGGCAGGCCCTTGCAGAACAGCAGGAGAAGATGCTGATTAATGGGGAAAG
  CGGCCAGGCCAAGCGGAACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_033437 unedited
  GTATGATTATGTAATACGATTTACTATAGGGCGGCCGCGAAATTCGCACCAGGCGGGGAA
  GCTGGGGTGAGGGACGAGTCCACGGGACAGCCCAAAGGCAACATGACGGAACCTTGCCAA
  AAGAAATGGTCAATGCATGGTTTGCTGAGAGAGTTCACACCATCCCTGTGTGCAAGGAAG
  GTATCAGAGGCCACACCGAATCTTGCTCTTGTCCCTTGCAGCAGAGTCCTCGTGCAGATA
  ACAGTGTCCCTGGAACACCAACCAGGAAAATCTCTGCCTCTGAATTTGACCGGCCTCTTA
  GACCCATTGTTGTCAAGGATTCTGAGGGAACTGTGAGCTTCCTCTCTGACTCAGAAAAGA
  AGGAACAGATGCCTCTAACCCCTCCAAGGTTTGATCATGATGAAGGGGACCAGTGCTCAA
  GACTCTTGGAATTAGTGAAGGATATTTCTAGTCATTTGGATGTCACAGCCTTATGTCACA
  AAATTTTCTTGCATATCCATGGACTGATATCTGCTGACCGCTATTCCCTGTTCCTTGTCT
  GTGAAGACAGCTCCAATGACAAGTTTCTTATCAGCCGCCTCTTTGATGTTGCTGAAGGTT
  CAACACTGGAAGAAGTTTCAAATAACTGTATCCGCTTAGAATGGAACAAAGGCATTGTGG
  GACATGTGGCAGCGCTTGGTGAGCCCTTGAACATCAAAGATGCATATGANGATCCTCGGT
  TCAATGCAGAAGTTGACCAAATTACAGGCTACAAGACACAAAGCATTCTTTGTATGCCAA
  TTAAGAATCATANGGNNAAGAGTNGTTGGTGTAGCCCAGGCCATCAACAAGAAATCAGNA
  AAACGTGGGACATTTACTGAANAAAGATGAAAAGACTNTGCTNGCTATTTGGCATTNTGN
  GGNATTNGTCTTATAATGCTCAGCTCTATGAGACTTACTGCTGNAGAACAAGAGAATANG
  TGCTGNCTGACTTGCTAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_033437 unedited
  AACTGTGNACCGCGGCCCGCTATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTGCTTCT
  ATTTTTTGTTTTTTTACCAGAATGATAAAAATAAAACTGCATTATGTCAAGATATTTGTA
  CACACTCAGAATTTTAAAAACTTAAGGAACATACTGAATTTTAGGCAAAATTTAGGGGAA
  TCGGGACATAGATGGGAAAACACATTTTGGCGAAAGGTCCTTGAGAATGCTGGATTACCA
  TGTCCCTTAGAAATTTTCTATCCAAATTAGTGATAGAAACTCAGATCAAATATCTTTTTT
  GTGGGTTGGAGGTAGAAGTTAACAAATCAAGCAAATCTCTCCTTCTACTCTCCCCAAATC
  CAAACATTCCTGCATATTTGAAACAAATTTTCCTTTCCTAGACCAGTTGCTTAATTCATT
  ATCTACTTCTCCAATATTGAGTAAATGTGTCTTTAGGGGCACACATGTAGAATATTAATC
  CACTTAAATGGGCTCAAATTCTAAGAAAATATTAACTATACTAAATGTTATGGGAAGAAA
  ATACATTGAATGTGAGTTAAGGGCCAGTGTTTAGCATTCACATCCAGGATTCTCTGGATC
  TCAGTTGACTTGTCCTATACATCAAAATATGGCAATTTTTCAATCACAATACTGCTAAAT
  GTTATTCAGGCATAAAGTTTCTTACCCACTGTTCCCTCAAATTCATTTTTGACCCTAGTA
  TTGGCAATAGCCCTTTGCTATTTATATAATTAAAACTTTNTCTTTAATTTCAATTGACAG
  GAAAAAGAGTCATTTTGTACTCTCTTATCTATACCATGTCTCACTTCAGTCAATGCTACA
  TTGTCACTTTTATTTTGAAAAGGAGGCTCAGAGAAAAAANCTCTAGAGAGATCTCTACAG
  ACATTTTNCTTCCTGATAGACATCCAGCCATTCATTCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_033437
• ORF Size: 2472 bp
• Insert Size: 4700
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_033437.2, NP_246273.2
• RefSeq Size: 6788
• RefSeq ORF: 2472
• Locus ID: 8654
• Domains: PDEase, GAF
• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism
• Gene Summary: This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream start codon, compared to variant 1. The encoded isoform (3, also known as PDE5A3) is shorter at the N-terminus, compared to isoform 1. There are no publicly available full-length transcripts supporting this variant; it is represented based on full-length cloning by RT-PCR in PMID:10679255. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.