SMG5 (NM_015327) Human Untagged Clone

CAT#: SC100799

SMG5 (untagged)-Human Smg-5 homolog, nonsense mediated mRNA decay factor (C. elegans) (SMG5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SMG5
• Synonyms: EST1B; LPTS-RP1; LPTSRP1; SMG-5
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100799 sequence for NM_015327 edited (data generated by NextGen Sequencing)
  ATGAGCCAAGGCCCCCCCACAGGGGAGAGCAGCGAGCCCGAAGCAAAAGTCCTCCACACT
  AAGCGGCTTTACCGGGCTGTGGTGGAGGCTGTGCATCGACTTGACCTCATCCTTTGCAAC
  AAAACTGCTTATCAAGAAGTATTCAAACCAGAAAACATTAGCCTGAGGAACAAGCTGCGT
  GAGCTCTGCGTCAAGCTTATGTTCCTGCACCCAGTGGACTATGGGAGAAAGGCTGAGGAG
  CTGCTGTGGAGAAAGGTATACTATGAAGTTATCCAGCTTATCAAGACTAACAAAAAGCAC
  ATCCACAGCCGGAGCACTTTGGAATGTGCCTACAGGACGCACCTGGTTGCTGGTATTGGC
  TTCTACCAGCATCTCCTTCTCTATATCCAGTCCCACTACCAGCTGGAACTGCAGTGCTGC
  ATCGACTGGACCCATGTCACTGACCCCCTCATAGGATGCAAGAAGCCAGTGTCTGCCTCA
  GGGAAGGAGATGGATTGGGCACAGATGGCATGTCACCGATGTCTGGTGTATCTGGGGGAT
  TTGTCCCGATATCAGAATGAATTAGCTGGCGTAGATACCGAGCTGCTAGCCGAGAGATTT
  TACTACCAAGCCCTGTCAGTAGCTCCTCAGATTGGAATGCCCTTCAATCAGCTGGGCACC
  CTGGCAGGCAGCAAGTACTATAATGTGGAAGCCATGTATTGCTACCTGCGCTGCATCCAG
  TCAGAAGTGTCCTTTGAGGGAGCCTATGGGAACCTCAAGCGGCTGTATGACAAGGCAGCC
  AAAATGTACCACCAACTGAAGAAGTGTGAGACTCGGAAACTGTCTCCTGGCAAAAAGCGA
  TGTAAAGACATTAAAAGGTTGCTAGTGAACTTTATGTATCTGCAAAGCCTCCTACAGCCC
  AAAAGCAGCTCCGTGGACTCAGAGCTGACCTCACTTTGCCAGTCAGTCCTGGAGGACTTC
  AACCTCTGCCTCTTCTACCTGCCCTCCTCACCCAACCTCAGCCTGGCCAGTGAGGATGAG
  GAGGAGTATGAGAGTGGATATGCTTTCCTCCCGGACCTTCTCATCTTTCAAATGGTCATC
  ATCTGCCTTATGTGTGTGCACAGCTTGGAGAGAGCAGGATCCAAGCAGTACAGTGCAGCC
  ATTGCCTTCACCCTGGCCCTCTTTTCCCACCTCGTCAATCATGTCAACATACGGCTGCAG
  GCTGAGCTGGAAGAGGGCGAGAATCCCGTCCCGGCATTCCAGAGTGATGGCACAGATGAA
  CCAGAGTCCAAGGAACCTGTGGAGAAAGAGGAGGAGCCAGATCCTGAGCCGCCTCCTGTA
  ACACCCCAAGTGGGTGAGGGCAGAAAGAGCCGTAAGTTCTCTCGCCTCTCCTGTCTCCGC
  CGTCGCCGCCACCCACCCAAAGTTGGTGATGACAGTGACCTGAGTGAAGGCTTTGAATCG
  GACTCAAGCCATGACTCAGCCCGGGCCAGTGAGGGCTCAGACAGTGGCTCTGACAAGAGT
  CTTGAAGGTGGGGGAACGGCCTTTGATGCTGAAACAGACTCGGAAATGAATAGCCAGGAG
  TCCCGATCAGACTTGGAAGATATGGAGGAAGAGGAGGGGACACGGTCACCAACCCTGGAG
  CCCCCTCGGGGCAGATCAGAGGCTCCCGATTCCCTCAATGGCCCACTGGGCCCCAGTGAG
  GCTAGCATTGCCAGCAATCTACAAGCCATGTCCACCCAGATGTTCCAGACTAAGCGCTGC
  TTCCGACTGGCCCCCACCTTTAGCAACCTGCTCCTCCAGCCCACCACCAACCCTCATACC
  TCGGCCAGCCACAGGCCTTGCGTCAATGGGGATGTAGACAAGCCTTCAGAGCCAGCCTCT
  GAGGAGGGCTCTGAGTCGGAGGGGAGTGAGTCCAGTGGACGCTCCTGTCGGAATGAGCGC
  AGCATCCAGGAGAAGCTTCAGGTCCTGATGGCCGAAGGTCTGCTTCCTGCTGTGAAAGTC
  TTCCTGGACTGGCTTCGGACCAACCCCGACCTCATCATCGTGTGTGCGCAGAGCTCTCAA
  AGTCTGTGGAACCGCCTGTCTGTGTTGCTGAATCTGTTGCCTGCTGCTGGTGAACTCCAG
  GAGTCTGGCCTGGCCTTGTGTCCTGAGGTCCAAGATCTTCTTGAAGGTTGTGAACTGCCT
  GACCTCCCCTCTAGCCTTCTGCTCCCAGAGGACATGGCTCTTCGTAACCTGCCCCCGCTC
  CGAGCTGCCCACAGACGCTTTAACTTTGACACGGATCGGCCCCTGCTCAGCACCTTAGAG
  GAGTCAGTGGTGCGCATCTGCTGCATCCGCAGCTTTGGTCATTTCATCGCCCGCCTGCAA
  GGCAGCATCCTGCAGTTCAACCCAGAGGTTGGCATCTTCGTCAGCATTGCCCAGTCTGAG
  CAGGAGAGCCTGCTGCAGCAGGCCCAGGCACAGTTCCGAATGGCACAGGAGGAAGCTCGT
  CGGAACAGGCTCATGAGAGACATGGCTCAGCTACGACTTCAGCTCGAAGTGTCTCAGCTG
  GAGGGCAGCCTGCAGCAGCCCAAGGCCCAGTCAGCCATGTCTCCCTACCTCGTCCCTGAC
  ACCCAGGCCCTCTGCCACCATCTCCCTGTCATCCGCCAACTGGCCACCAGTGGCCGCTTC
  ATTGTCATCATCCCAAGGACAGTGATCGATGGCCTGGATTTGCTGAAGAAGGAACACCCA
  GGGGCCCGGGATGGGATTCGGTACCTGGAGGCAGAGTTTAAAAAAGGAAACAGGTACATT
  CGCTGCCAGAAAGAGGTGGGAAAGAGCTTTGAGCGGCATAAGCTGAAGAGGCAGGATGCA
  GATGCCTGGACTCTCTATAAGATCCTAGACAGCTGCAAACAGCTGACTCTGGCCCAGGGG
  GCAGGTGAGGAGGATCCGAGTGGCATGGTGACCATCATCACAGGCCTTCCACTGGACAAC
  CCCAGCGTGCTTTCAGGCCCCATGCAGGCAGCCCTGCAGGCCGCTGCCCACGCCAGTGTG
  GACATCAAGAATGTTCTGGACTTCTACAAGCAGTGGAAGGAAATTGGTTGA
  
  Clone variation with respect to NM_015327.2
  1311 t=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015327 unedited
  NGTCTTACCCGCCCGTTGCCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAA
  GCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG
  CGAATTCGGCACGAGGGCCTCACAGCGATGGCGGCCGAGCAGGGCCGGTGGCGGCGGCGG
  CTGCGGCTACGGCCGGAGACGGCAGTGTTGGCGGTAGTGGTGGGTGGCAGGGGCCTGTGA
  CCGGGAGCTGCCCCCGGACCCGGGCACCATGAGCCAAGGCCCCCCCACAGGGGAGAGCAG
  CGAGCCCGAAGCAAAAGTCCTCCACACTAAGCGGCTTTACCGGGCTGTGGTGGAGGCTGT
  GCATCGACTTGACCTCATCCTTTGCAACAAAACTGCTTATCAAGAAGTATTCAAACCAGA
  AAACATTAGCCTGAGGAACAAGCTGCGTGAGCTCTGCGTCAAGCTTATGTTCCTGCACCC
  AGTGGACTATGGGAGAAAGGCTGAGGAGCTGCTGTGGAGAAAGGTATACTATGAAGTTAT
  CCAGCTTATCAAGACTAACAAAAAGCACATCCACAGCCGGAGCACTTTGGAATGTGCCTA
  CAGGACGCACCTGGTTGCTGGGTATTGGCTTCTACCAGCATCTCCTTCTCTATATCCAGT
  CCCACTACCAGCTGGAACTGCAGTGCTGCATCGACTGGACCCATGTCACTGACCCCTCAT
  AGGATGCAAGAGCCAGCGTCTGCCTCAGGGAAGGAGATGGACTTGGCCCAGATGGCATGC
  ACCCGATGTCTGGGGTTCTGCGGGAATCTCCCCCACACCACAACGATTACCCCGCCCATT
  CCCCCATCTGCTTCCCGCCCATTCTCCTCCCCCCCGTGTCCCACTCCTCTTATCGCCCCC
  CTTTCCTCACTCTCCCCCCGCTCGCCCTCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015327 unedited
  NTTTTTAGCTNTGGNACCGCGGCCGCATNCTAGNGATCGGTTTTTTTTTTTTTTTTTTGG
  GCTTGGGGGGTTTATTAAGTGATGCTTTAGTCTCAGTCTCTGCCAGCAACTGGGAGTGGG
  GTGACTCCACTCACCCCAGATTTCCCAGACTTGCTATTTTAGAGGAGAGAGGCAGGAAGC
  CAACTATCCTCTAAGCCACAGCTTGGGAAGCTAGGCTAGTACTGGGGTGGGGGCAGCAGA
  GCTGAGACCCTCCACCCCGAGCCCCTAGCCTGTGCTATCCTCCCAGCCTGAGGGGGAGGA
  GCTGAGGCAATCCTGGCTGCAGCCTCCCACACACAGCCCTGCTCTTGGTGCGCCATTCAC
  TGCCCTGAGCTATTCATGATCTCTGCTCCCAGATATTCACCTCAACACTCCAAAAGCCAG
  CCCCTTCAGGTCTTCAGTCCTGCGGAAGGCAAAAGGAGGGACGGGGGCCTCTGACTGAGC
  AGCTTCAAGGAGCCTCTCCTTTCTGCTGCCCACTGAATGCCGGCCCCTTGTCTTCAGCCC
  TCCCTTAGATAGGAAAGGGGGTGGGTGGCCTCAGACTGCACCCNCTTTCTTCTCTTCCTG
  GCATCCCATTCTGTCCCAGCACAGCAGCCAAGAGCACANAGCACAGCACCTGTGGGGGCT
  GGTGGGCACAGGAGACCGTGCTGGCACAGGCCAGACTGCCTGCAGCCCCTAGTGGGGCCT
  GGCTCCTGCCCTGGNAGCTAGACAGAGGGAGACACAAAGCAGAAGTGGGGCAATGGGATG
  TGCCAGCCCTGCAGCAGGAGGAGGAGCCCACGACCCTGACCTGCTACTCGGTGCAGGCAC
  TNCAGGCCTTGCTCTCTACAGCCCCTGGTGCTGGCTCCCAAGCTGCTGGTCCCCATAAGG
  GGGCTGAAGGAGAAGGGTAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015327
• ORF Size: 3051 bp
• Insert Size: 5000
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_015327.1, NP_056142.1
• RefSeq Size: 4578
• RefSeq ORF: 3051
• Locus ID: 23381
• Domains: PINc
• Gene Summary: SMG5 is involved in nonsense-mediated mRNA decay (Ohnishi et al., 2003 [PubMed 14636577]). [supplied by OMIM, Mar 2008]