SHFM3 (FBXW4) (AK056917) Human Untagged Clone

CAT#: SC101935

(untagged)-Human cDNA FLJ32355 fis, clone PROST2007956, highly similar to Human dactylin mRNA

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FBXW4
• Synonyms: DAC|FBW4|FBWD4|SHFM3|SHSF3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AK056917, the custom clone sequence may differ by one or more nucleotides
  
  
  AGTGGTTAGGTGAGAGAAAAGGGCAGCTCTGATCTCCAGCAGGATTAAAGTTGCTCCCCTCTCTATTGAT
  TTACCTTGATGCTAATTTAGGCCTGTGGCCAGTCAGAGGCCTGCCATAGGCCTTAAGAGATGCCTGGCCG
  GGCGCGTTGGCTCACGCCTGTAATTCCAGCACTTTCGGAGGCCAAGGTGGGCGGATCACGAGGTCAAGAA
  ATCGAGACCAGACTGGCGAGCATGGTGAAACCCTGTCTCTGCTAGATATGCAAGAATTGGCTGGGTGTGG
  TGGTGCATGCCCGTGGTCCCAGCAACTCGGGAGGCTGAGGCAGGGGAACTGCTTGAACCCAGGAGGTGGA
  GGTTGCAGTGAGCCAAGATTGCACCACTGCACTCCAGCCTGGTGACAGAGGGAGATTCCTCTCAAAAAAA
  AAAGAGATGCCTGTGTCTGTGCTGGCGCCCTTCACCTGGTCCCCACTCCTCAAATCTGGAGAGTCCAATT
  GCCTGCTCGAATGATTTCCAAGTGGCTATTATGTTTCCAGCCCAAGGTAATTGGCTTTACCAGTTATTAA
  TGGATTACCCTAAATAGAAAAATGTTTGGGGAGGGAGGAGAGGGCAACACCTCCTGACTTTGTAGTTTGG
  TTCTTCAGTATCCACTGGCTGCTTCCCAGGCTGGCAGTCACTTGGGGCATGGCACAGTGACTGTTTTGGC
  ACTCCCACTCTATAGTTTAGTGGCTGCTGGCAGCCTTGAAGAAGCTAGGATGATGAGGCATGTCCCTGGA
  GGAAGTCTTGGCTTGAGTCATGAAAGCCCCAGGATAGCTCGTATGGCCTTTTTATCAGCCTAGGGCTGAA
  GAGCAGACACCTGCCCTGCAATTTTTATTGTAAATCAGTGGTTTGGCCAGTGCCTGCCCTAACATCTCCC
  CCATCAGCAACTGGCAGGCCAACCTCCCCACCCCAGCCAAAGCCACTGGCTTCTCCAGGGCCCTCTCTGT
  GGAAATCCATCTCCTCACTGACATGCTTGGGATCAGAGGAGCCACAGGTGCTGGGCTGGGCCACAGCAGA
  TGGGCTGTTGCCCCCACATTGCTAATGTGTGATTAGTATGTCCAGTCTTCCTTCCCCCCCATGAAAGACA
  TGCAGGGACATGGCCCATTCCTAACACCACTGCTTTCAACTTGCTTGCTTGCTGTTTAAGTTTCAAGCCC
  AGGTTTCCTGAAGGCTTCCCCTCTCCACCCTACCAAATCCTCTAACAAGAGTTGTCTGGCTCATAGGTTC
  AAGTTCTAGGCCAGCACTACAGCCAGCCTGGCCCACTGCGTGGATCCTTTATACCAGGCTTGTGCCTCCA
  TTTAAAGTGAGACTCTGCAGATTGTATATGTTGAGGTTTTTAGGGCTCTAAAGGGACACTCTGGGCAACA
  GGGTCAGCCTGCTCCTTCTCGTGTGCCTCTCGGCGTTGTTTGTTATTGGCCTCAGAGGACTCTGGCGGAC
  TCCCATCCCACTCTCCAGAAGCCCTGGCTGCCTGGCTTCTGGGGACTCCGCCCCACCTATGTAGTAGCAT
  ATACCCATCCAAGGGATTCACTCATCTTTCATATTTTTTCCTTTTCTCATTTTGGTGCCTTTATACCTGC
  CCTGTGCTTATACCACATTACCAGATTCCCCCAGCTCTTAAAACCTTCAGGAATGCCCCTCAGCTGCTCC
  TACTTCTGACCACGGGCAGCACCTTCCAGCCCCAGCAACCTGTTCCCTGGAGCCCTTTTCTGTGCCCTGC
  CATTGTGCTCTTGGGATTCCTAAACAGGAGCCCGACAGGCTCGCTCTCTCCTGGGCTCAAACTTGTCACG
  TCTAGAGATCTGTTTTTATGGTGTGCAGAGACCACTCAACCTAGTCTGTGCAGCAGGCCCAGCTGTGGCT
  GAGCTCGTGGGCCTTCTGGCCAGGCACCACTGGGGGCTACACCCCTCACAGTGCTCCAAGATGTCTTCCC
  ACAGCTCCTCTGATGGCTCCCTTGGCCACTTCTCTCATTCTGGCTTAACCCACAATCCCCTAGGGAATGA
  GCACGGCATTTCCCATTGTTAGCCATTTCCACCACAGGTTCTGACAAGCCAGATCTGACTTTGTTCTCCT
  TTTGTTTGGCAGCTCTTTTGTGACAGGGACGGCTTGTTGCGGGCACTTCTCACCCCTGAGAATCTGGGAC
  CTCAACAGTGGGCAGCTGATGACACACTTGGGCAGTGACTTTCCCCCAGGGGCTGGGGTGCTGGATGTCA
  TGTATGAGTCCCCTTTCACACTGCTGTCCTGTGGCTATGACACCTATGTTCGCTACTGGGACCTCCGCAC
  CAGCGTCCGGAAATGTGTCATGGAGTGGGAGGAGCCCCACGACAGCACCCTGTACTGCCTGCAGACAGAT
  GGCAACCACCTGCTGGCCACAGGTTCCTCCTACTACGGTGTTGTACGGCTGTGGGACCGGCGTCAAAGGG
  CCTGCCTGCACGCCTTCCCGCTGACGTCGACTCCCCTCAGCAGCCCTGTGTACTGCCTGCGTCTCACCAC
  CAAGCATCTCTATGCTGCCCTGTCTTACAACTTCCACGTCCTGGATTTTCAAAACCCATGACCGTCAGGG
  CCACCCCTGCCTCTGGGCCAGGGAAACCAGCTACTCAGGGACTTCTCTTGCCTGGAGGGTGCAGTGATAG
  CTCCTCCTCACTGCCCCACTGTGCTCCTGGGCCTGTGACCCCAGTGCTCAGGCACCTTGCACTAGAGGCT
  TCTGACTCCTGGGACTTTGGAGCTTACCAGAGATGCAGTCCCTCCCAGGAACCTGTTGGAGAGGCAGGAC
  CTGCTGCTTTAGAGTGCGGCTGAACCCGGGCCTTGCGTCCCTGTTTGGCCAGAGCAAGGATCTGGCCTGG
  AGAGGCCCATCCTATACCCCTTATTAGAGCCATGACAGCCTACAGAGTGAGGTGAGGTGCTCCCACCTTC
  CCAGATGGTTCCTTTCTGCCCCTTCCTGGAAGGAAAGGTGAGGCTGCCAATAGCCTCCTGGCACCAGCCA
  GACCTCACCCTTGACCAACCTCTCGGGGCTGGGGGTTCATTCCTGGGGCACTGTGGCCTGGTTTTGCTTT
  GAAACCAAGAAAGAGCAAAGGGAACCCAGCAGTTCTGAGTGAGTTCTGAGCCAGCCCTACCTCAGGCTGG
  CTGTTGAGACATGCTACAATTTTCATTTTTGTAAAAATAAAGCTTGATTGTTCAC
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AK056917 unedited
  GTAACGTCAGAATTGTAAACGACTCCTAAAGGCGGCCGCGAATTCGGCACGAGGGCTGGG
  GTGCTGGATGTCATGTATGAGTCCCCTTTCACACTGCTGTCCTGTGGCTATGACACCTAT
  GTTCGCTACTGGGACCTCCGCACCAGCGTCCGGAAATGTGTCATGGAGTGGGAGGAGCCC
  CACGACAGCACCCTGTACTGCCTGCAGACAGATGGCAACCACCTGCTGGCCACAGGTTCC
  TCCTACTACGGTGTTGTACGGCTGTGGGACCGGCGTCAAAGGGCCTGCCTGCACGCCTTC
  CCGCTGACGTCGACTCCCCTCAGCAGCCCTGTGTACTGCCTGCGTCTCACCACCAAGCAT
  CTCTATGCTGCCCTGTCTTACAACCTCCACGTCCTGGATTTTCAAAACCCATGACCGTCA
  GGGCCACCCCTGCCTCTGGGCCAGGGAAACCAGCTACTCAGGGACTTCTCTTGCCTGGAG
  GGTGCAGTGATAGCTCCTCCTCACTGCCCCACTGTGCTCCTGGGCCTGTGACCCCAGTGC
  TCAGGCACCTTGCACTAGAGGCTTCTGACTCCTGGGACTTTGGAGCTTACCAGAGATGCA
  GTCCCTCCCAGGAACCTGTTGGAGAGGCAGGACCTGCTGCTTTAGAGTGCGGCTGAACCC
  GGGCCTTGCGTCCCTGTTTGGCCAGAGCAAGGATCTGGCCTGGAGAGGCCCATCCTATAC
  CCCTTATTAGAGCCATGACAGCCTACAGAGTGAGGTGAGGTGCTCCCACCTTCCCAGATG
  GTTCCTTTCTGCCCCTTCCTGGAAGAAAAGTGAGGCTGCCAATAGCCTCCTGGCACCAGC
  CAGACCTCACCCTTGACCAACCTCTCGGGGCTGGGGGGTTCATTCCTGGGGCACTA
  
• Restriction Sites: NotI-NotI
• ACCN: AK056917
• Insert Size: 1200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AK056917.1
• RefSeq Size: 3205 bp
• RefSeq ORF: 3205 bp
• Locus ID: 6468
• Cytogenetics: 10q24.32
• Protein Families: Druggable Genome
• Gene Summary: 'This gene is a member of the F-box/WD-40 gene family, which recruit specific target proteins through their WD-40 protein-protein binding domains for ubiquitin mediated degradation. In mouse, a highly similar protein is thought to be responsible for maintaining the apical ectodermal ridge of developing limb buds; disruption of the mouse gene results in the absence of central digits, underdeveloped or absent metacarpal/metatarsal bones and syndactyly. This phenotype is remarkably similar to split hand-split foot malformation in humans, a clinically heterogeneous condition with a variety of modes of transmission. An autosomal recessive form has been mapped to the chromosomal region where this gene is located, and complex rearrangements involving duplications of this gene and others have been associated with the condition. A pseudogene of this locus has been mapped to one of the introns of the BCR gene on chromosome 22. [provided by RefSeq, Jul 2008]'