HEF1 (NEDD9) (NM_182966) Human Untagged Clone

CAT#: SC107603

NEDD9 (untagged)-Human neural precursor cell expressed, developmentally down-regulated 9 (NEDD9), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NEDD9
• Synonyms: CAS-L; CAS2; CASL; CASS2; HEF1
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_182966, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAGTATAAGAATCTTATGGCAAGGGCCTTATATGACAATGTCCCAGAGTGTGCCGAGGAACTGGCCT
  TTCGCAAGGGAGACATCCTGACCGTCATAGAGCAGAACACAGGGGGACTGGAAGGATGGTGGCTGTGCTC
  ATTACACGGTCGGCAAGGCATTGTCCCAGGCAACCGGGTGAAGCTTCTGATTGGTCCCATGCAGGAGACT
  GCCTCCAGTCACGAGCAGCCTGCCTCTGGACTGATGCAGCAGACCTTTGGCCAACAGAAGCTCTATCAAG
  TGCCAAACCCACAGGCTGCTCCCCGAGACACCATCTACCAAGTGCCACCTTCCTACCAAAATCAGGGAAT
  TTACCAAGTCCCCACTGGCCACGGCACCCAAGAACAAGAGGTATATCAGGTGCCACCATCAGTGCAGAGA
  AGCATTGGGGGAACCAGTGGGCCCCACGTGGGTAAAAAGGTGTTCCAGAGAGATGGGCAAGTGTCCTATT
  TCTTAGTGAGAGCCTCTAAACAAACCAGCTTGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_182966 unedited
  AATAACACCCGCCCGTTGAAGCAAAGGGCGGTAGGCGTGTACGGTGGGCAGGTCTATATA
  AGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCC
  GCGAATTCGGCACGAGGCGACTGACAAGCGGCTCTGCCCGGGACCTTCTCGCTTTCATCT
  AGCGCTGCACTCAATGGAGGGGCGGGCACCGCAGTGCTTAATGCTGTCTTAACTAGTGTA
  GGAAAACGGCTCAACCCACCGCTGCCGAAATGAAGTATAAGAATCTTATGGCAAGGGCCT
  TATATGACAATGTCCCAGAGTGTGCCGAGGAACTGGCCTTTCGCAAGGGAGACATCCTGA
  CCGTCATAGAGCAGAACACAGGGGGACTGGAAGGATGGTGGCTGTGCTCATTACACGGTC
  GGCAAGGCATTGTCCCAGGCAACCGGGTGAAGCTTCTGATTGGTCCCATGCAGGAGACTG
  CCTCCAGTCACGAGCAGCCTGCCTCTGGACTGATGCAGCAGACCTTTGGCCAACAGAAGC
  TCTATCAAGTGCCAAACCCACAGGCTGCTCCCCGAGACACCATCTACCAAGTGCCACCTT
  CCTACCAAAATCAGGGAATTTACCAAGTCCCCACTGGCCACGGCACCCAAGAACAAGAGG
  TATATCAGGTGCCACCATCAGTGCAGAGAAGCATTGGGGGAACCAGTGGGCCCCACGTGG
  GTAAAAAGGTGATAACCCCCGTGAGGACAGGCCATGGCTACGTATACGAGTACCCATCCA
  GATACCAAAAGGACGTCTATGAGATCCCTCCTTCTCATACCACTCAAGGGGTATACGACA
  TCCCTCCCTCATCAGCAGAGGCCCCTGTGTTTCAGTTCCAGTNGCAGAATACAACTCAGG
  GNGTGTATGACATCCTGCTACACAGGNGTATATGCCATNCCGCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_182966
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182966.1, NP_892011.1
• RefSeq Size: 1329 bp
• RefSeq ORF: 1329 bp
• Locus ID: 4739
• Cytogenetics: 6p24.2
• Gene Summary: 'The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]'
  Transcript Variant: This variant (2) lacks several exons and includes an alternate 3' terminal exon, compared to variant 1. It encodes isoform 2 which is shorter and has a distinct C-terminus, compared to isoform 1.