PDPK1 (NM_031268) Human Untagged Clone

CAT#: SC107892

PDPK1 (untagged)-Human 3-phosphoinositide dependent protein kinase-1 (PDPK1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDPK1
• Synonyms: PDK1; PDPK2; PDPK2P; PRO0461
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_031268, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCAGGACCACCAGCCAGCTGTATGACGCCGTGCCCATCCAGTCCAGCGTGGTGTTATGTTCCTGCC
  CATCCCCATCAATGGTGAGGACCCAGACTGAGTCCAGCACGCCCCCTGGCATTCCTGGTGGCAGCAGGCA
  GGGCCCCGCCATGGACGGCACTGCAGCCGAGCCTCGGCCCGGCGCCGGCTCCCTGCAGCATGCCCAGCCT
  CCGCCGCAGCCTCGGAAGAAGCGGCCTGAGGACTTCAAGTTTGGGAAAATCCTTGGGGAAGGCTCTTTTT
  CCACGGTTGTCCTGGCTCGAGAACTGGCAACCTCCAGAGAATATGCGACCAGGGCCAACTCATTCGTGGG
  AACAGCGCAGTACGTTTCTCCAGAGCTGCTCACGGAGAAGTCCGCCTGTAAGAGTTCAGACCTTTGGGCT
  CTTGGATGCATAATATACCAGCTTGTGGCAGGACTCCCACCATTCCGAGCTGGAAACGAGTATCTTATAT
  TTCAGAAGATCATTAAGTTGGAATATGACTTTCCAGAAAAATTCTTCCCTAAGGCAAGAGACCTCGTGGA
  GAAACTTTTGGTTTTAGATGCCACAAAGCGGTTAGGCTGTGAGGAAATGGAAGGATACGGACCTCTTAAA
  GCACACCCGTTCTTCGAGTCCGTCACGTGGGAGAACCTGCACCAGCAGACGCCTCCGAAGCTCACCGCTT
  ACCTGCCGGCTATGTCGGAAGACGACGAGGACTGCTATGGCAATTATGACAATCTCCTGAGCCAGTTTGG
  CTGCATGCAGGTGTCTTCGTCCTCCTCCTCACACTCCCTGTCAGCCTCCGACACGGGCCTGCCCCAGAGG
  TCAGGCAGCAACATAGAGCAGTACATTCACGATCTGGACTCGAACTCCTTTGAACTGGACTTACAGTTTT
  CCGAAGATGAGAAGAGGTTGTTGTTGGAGAAGCAGGCTGGCGGAAACCCTTGGCACCAGTTTGTAGAAAA
  TAATTTAATACTAAAGATGGGCCCAGTGGATAAGCGGAAGGGTTTATTTGCAAGACGACGACAGCTGTTG
  CTCACAGAAGGACCACATTTATATTATGTGGATCCTGTCAACAAAGTTCTGAAAGGTGAAATTCCTTGGT
  CACAAGAACTTCGACCAGAGGCCAAGAATTTTAAAACTTTCTTTGTCCACACGCCTAACAGGACGTATTA
  TCTGATGGACCCCAGCGGGAACGCACACAAGTGGTGCAGGAAGATCCAGGAGGTTTGGAGGCAGCGATAC
  CAGAGCCACCCGGACGCCGCTGTGCAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_031268 unedited
  CAAATTTTGTATACGAACTCACTATAGGGCGGCCGCGACTTCGGCACGAGGCACAGGAGG
  GCGAGAACAGCACACTTCAACCCCAGCTTGCTGGTCGGCTTTCCTCTAGAGAGAGCCGGT
  TTTGGGGCCATTTCCCTTTGATGCTTTGGTGGCCTTGCCCCGCTCTGCAGCACAGACAGG
  CCAGATGCATTTGTCCTTTGCCTAGCTACTCCCCAGGTAGAGAGTGCTCCTGGTGGCCTG
  GCAGGTCTGGGCCCTTCTCTCCCTGCCCAGGTTGTCCCTGGAGGGCAGCCCTCACTCCCT
  TTNGGGGGAGAGGCAGACATTGCTGCCCACAGACCTGCCTCTGACTCAACTGTGTCCACC
  CTCCCTGGTCCCTACCCCCAAGTCACAGGTGACTCAGCAGTGACCCTGTGTGCCAGGCCA
  GATCCAAACTGAGAGGGAAGGTGTCGTTTTTACACTGCTAATGACGAGAGTGGCTCTTTT
  TAGCTAGGCGAGTACAGACGGGGCCTGGGAGGGGGCAGAGATGTTCCCCAGGCCCTGCCT
  GTGGTTCCTGCCTGGGCCTTGGCTGCTGCTGTGTGAGAGCTGCATGTGAGCCTGTGACCG
  TGAGCTGGGGTGAGCTGGGCCGCACCTCCCCTGGGGCCCCAGGGAGCAGGACGCTTCCGG
  GGCCCAGCACGTTGCCCTGGGCCTGTGGCCGGAGTCGGAGTCCTCTCTCCTCCTCCTGGC
  TTTTGGAAGGCTTGGCTGTGTGGGGGAGTCTCTCTTACCCTTTCAGGATTTCTGGTCAGG
  CTTCCTNCTCTCANAGCTATTTACCCATTCAGACGTNCTGTGCTCCTGTAGGAGAGTGCT
  CTCTCGATCTCCAGGGCGTCTGTTATAAGAAACAAGTGACAAGGCGTGGCTTTATTGAGC
  CTCGCTGGCTGCTGGGGGACTCTTCCGGCGTCTTCTTTGAAGAACACACTTCTGGCGTAC
  TGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_031268 unedited
  CGCGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTACACTCTACATGCT
  TTTTATTACAAGACTACTGACCATACGAGGAAAATTCATCATCTGGTAACTACCCCTAAA
  GCACAAGTATTTGGAAAAAAAAAAAGGATAAACAGATTCATTAAATTATATGATTAAATC
  ACTGACCCAATAGAAAATTTATATAATAAAGCCAGAAAAAAAGTTGTAAAATATAGTTTA
  CAATAATTATTCACATTCAAGGCTGTACATCAATACATACAACAACGTGGCCCCGAACAT
  GAATTCAATTCAAATAATTCATATATTAGAATTTAAATAACACAGACTGAACTAGAGGCC
  AACAATAGCCAGCAAATAACCTTATATAAGAATAAAAATACAAAAGTGTATATCCTAGTA
  TCATTGCATTTGTTTTCATAAGTTCTTTTAATTTTTGCTTTGTCTGTACATCACAGTTAC
  AGCTACAGACCAGGTAGAGAATATTACGCATGTTCTGCTTGCCACCCACCTGAAAGACGT
  ACTAGTCATTTATCCTATTTTATGTTGTGAAGGAACAGCTACAGCAGCTCTAAATACTGT
  AACAATTTAATTTTGCCAACATCCAACTTACTTACAGTCGTAATAGAGGCTTTTACCAGC
  ACAATGACCCCTATTCGTAGTCATTCTGCCAGGTGGTTAGGCTATGTGGGTTACACAGCT
  TAAAAAAGGTCTGCCTTTTGTAAAAAGTTCTCTGTCCATAGTTTGGGGTTTCTTCCTATG
  GAAGAAAAGANTTACGGGGAAAATTCCGGTACCCCCTAGGCTTTGTAAATTGCCTACCAC
  AGGCCTCTCAACAAATGAAGTGCTCTTTGGTGAAACACTTTCTTCCTCACCCAGTCTTCC
  CAGGAAGCCCATTTAACAACTTGGTTTTTTGGCCACAAGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_031268
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031268.4, NP_112558.2
• RefSeq Size: 6873 bp
• RefSeq ORF: 1290 bp
• Locus ID: 5170
• Cytogenetics: 16p13.3
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Endometrial cancer, Focal adhesion, Insulin signaling pathway, mTOR signaling pathway, Non-small cell lung cancer, PPAR signaling pathway, Prostate cancer
• Gene Summary: ''
  Transcript Variant: This variant (2) lacks three consecutive exons in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.