MID1 (NM_000381) Human Untagged Clone

CAT#: SC109438

MID1 (untagged)-Human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MID1
• Synonyms: BBBG1; FXY; GBBB1; MIDIN; OGS1; OS; OSX; RNF59; TRIM18; XPRF; ZNFXY
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109438 sequence for NM_000381 edited (data generated by NextGen Sequencing)
  ATGGAAACACTGGAGTCAGAACTGACCTGCCCTATTTGTCTGGAGCTCTTTGAGGACCCT
  CTTCTACTGCCCTGCGCACACAGCCTCTGCTTCAACTGCGCCCACCGCATCCTAGTATCA
  CACTGTGCCACCAACGAGTCTGTGGAGTCCATCACCGCCTTCCAGTGCCCCACCTGCCGG
  CATGTCATCACCCTCAGCCAGCGAGGTCTAGACGGGCTCAAGCGCAACGTCACCCTACAG
  AACATCATCGACAGGTTCCAGAAAGCATCAGTGAGCGGGCCCAACTCTCCCAGCGAGACC
  CGTCGGGAGCGGGCCTTTGACGCCAACACCATGACCTCCGCCGAGAAGGTCCTCTGCCAG
  TTTTGTGACCAGGATCCTGCCCAGGACGCTGTGAAGACCTGTGTCACTTGTGAAGTATCC
  TACTGTGACGAGTGCCTGAAAGCCACTCACCCGAATAAGAAGCCCTTTACAGGCCATCGT
  CTGATTGAGCCAATTCCGGACTCTCACATCCGGGGGCTGATGTGCTTGGAGCATGAGGAT
  GAGAAGGTGAATATGTACTGTGTGACCGATGACCAGTTAATCTGTGCCTTGTGTAAACTG
  GTTGGGCGGCACCGCGATCATCAGGTGGCAGCTTTGAGTGAGCGCTATGACAAATTGAAG
  CAAAACTTAGAGAGTAACCTCACCAACCTTATTAAGAGGAACACAGAACTGGAGACCCTT
  TTGGCTAAACTCATCCAAACCTGTCAACATGTTGAAGTCAATGCATCACGTCAAGAAGCC
  AAATTGACAGAGGAGTGTGATCTTCTCATTGAGATCATTCAGCAAAGACGACAGATTATT
  GGAACCAAGATCAAAGAAGGGAAGGTGATGAGGCTTCGCAAACTGGCTCAGCAGATTGCA
  AACTGCAAACAGTGCATTGAGCGGTCAGCATCACTCATCTCCCAAGCGGAACACTCTCTG
  AAGGAGAATGATCATGCGCGTTTCCTACAGACTGCTAAGAATATCACCGAGAGAGTCTCC
  ATGGCAACTGCATCCTCCCAGGTTCTAATTCCTGAAATCAACCTCAATGACACATTTGAC
  ACCTTTGCCTTAGATTTTTCCCGAGAGAAGAAACTGCTAGAATGTCTGGATTACCTTACA
  GCTCCCAACCCTCCCACAATTAGAGAAGAGCTCTGCACAGCTTCATATGACACCATCACT
  GTGCATTGGACCTCCGATGATGAGTTCAGCGTGGTCTCCTACGAGCTCCAGTACACCATA
  TTCACCGGACAAGCCAACGTCGTTAGTCTGTGTAATTCGGCTGATAGCTGGATGATAGTA
  CCCAACATCAAGCAGAACCACTACACGGTGCACGGTCTGCAGAGCGGCACCAAGTACATC
  TTCATGGTCAAGGCCATCAACCAGGCGGGCAGCCGCAGCAGTGAGCCTGGGAAGTTGAAG
  ACAAACAGCCAACCATTTAAACTGGATCCCAAATCTGCTCATCGAAAACTGAAGGTGTCC
  CATGATAACTTGACAGTAGAACGTGATGAGTCATCATCCAAGAAGAGTCACACACCTGAA
  CGCTTCACCAGCCAGGGGAGCTATGGAGTAGCTGGAAATGTGTTTATTGATAGTGGCCGG
  CATTATTGGGAAGTGGTCATAAGTGGAAGCACATGGTATGCCATTGGTCTTGCTTACAAA
  TCAGCCCCGAAGCATGAATGGATTGGGAAGAACTCTGCTTCCTGGGCGCTCTGCCGCTGC
  AACAATAACTGGGTGGTGAGACACAATAGCAAGGAAATCCCCATTGAGCCTGCCCCCCAC
  CTCCGGCGCGTGGGCATCCTGCTGGACTATGATAACGGCTCTATCGCCTTTTATGATGCT
  TTGAACTCCATCCACCTCTACACCTTCGACGTCGCATTTGCGCAGCCTGTTTGCCCCACC
  TTCACCGTGTGGAACAAGTGTCTGACGATTATCACTGGGCTCCCTATCCCAGACCATTTG
  GACTGCACAGAGCAGCTGCCGTGA
  
  Clone variation with respect to NM_000381.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000381 unedited
  GTCAAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGNNGTTGGGAAA
  CTCATCCATCAAACCGTCACCTCTGATTCTATGCAGGGTCACTATGAAAGAGGCAGCCTG
  CCCCAGTTTGGGTATTCTGAAGTGTTAGTATGTAGTGGAGCTGGTGGTGTGAAGCTGATT
  CATTTTGTTTCAAAGCCCCAAATGGCATGCTGCCGAACACAGGCACAAGCACAGCAGCGT
  CTCCTTAGAAATAATGACTCCAAGGCAAACAGCCCTCATTGCAAAATGTAGAAGACTGCC
  ATCTGCTTGTCTCCACTGTGGTTTATTCGTTTCAGGGTTTTAAATGACGTAATAAAAAGA
  TAGCTGATCAGCTTCCTTGGGTTTTGCTGATGACACAAGAGAGCTTTGCCTGAAGATGGA
  AACACTGGAGTCAGAACTGACCTGCCCTATTTGTCTGGAGCTCTTTGAGGACCCTCTTCT
  ACTGCCCTGCGCACACAGCCTCTGCTTCAACTGCGCCCACCGCATCCTAGTATCACACTG
  TGCCACCAACGAGTCTGTGGAGTCCATCACCGCCTTCCAGTGCCCCACCTGCCGGCATGT
  CATCACCCTCAGCCAGCGAGGTCTAGACGGGCTCAAGCGCAACGTCACCCTACAGAACAT
  CATCGACAGGTTCCAGAAAGCATCAGTGAGCGGGCCCAACTCTCCCAGCGAGACCCGTNC
  GGAGCGGGCCTTTGACGCCAACACCATGACCTCCGCGGAGAAGGTCTCTGCCAGTTNTGT
  GACCAGNATCCTGCCCGGNACCTTGTGAGNACCTGTGTACTTTGTGAAGATCCTATGGGG
  ACGAGTGCCTGAAGCCACTCACCGAATAAGAGCCTTTACAGGCATGGTCTGATTGACCAT
  TCGACTTACTTCCGGGGGCCTGATGTGCTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000381 unedited
  ATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGTCTTACAACTCT
  ATTGTAAACTATACTAGACTATAGAGGGACTTCTACATCTTTCAAGATGTGTTTAATAAA
  GGTCTGTTTATAATAACTTTTGAGGCATGAATCTAGCAAATAGTACTTTATACAATGTCC
  CTTGTCATTACCAACTCATAAATATTAAGTGTTTTTCAGTGACTTATGTTTGGATGTGGT
  AGTGCTGATCAGGGCCATGTGCTGATGTCCTGGAGAGCAAAATCAATCCAAAGTGGTGCT
  GCTATTTGTGACAGAACATGTTTATTTACTCAGCCCCGGAGACAAAAGGAAAATTGATAT
  GGGGGAGCGGGAAATAGGAGAACTATTAAATGTAGTGAAGAAATTTCACAGGTCTAAAGG
  AACTATTAAAAGGAAGGATAAAGTAGATTCTATACTATAAAACAGAATCCTACCTCTGAT
  AAAAGACAAATCAGCCTGAATTTTTGAATAATCAATAGGATTCAAAATGACTATTTTCAA
  TTGCAATCTCATTCTTAATGTTCTTCCAGAACCCTTAACCCTGAGAGAAGCAGGGCAAAA
  TGTGGCCAAAGGATTCAGAACCTTTCTTTCCAACATTATCCCATCAGCTATGAGAAGGTA
  AAGATTTAGCCACGGCAAGGTCCGAGGTCCAAAATCAACTCTTTTCATTCTCTGTCTTGC
  TCTCCATTNTAGTTGATCTGATACAACTTTTTGACATCACAATTAAAACACTTCAATTAA
  ACCAGAGTAAGAAAGCCTGTTTCCTCCCCTACTANGNTCAACACATNNCTGTATGCACCA
  TCGAGTGANAAGACTACATGGNNATCATCATGGATATCAGTAGAAGAGAGTACATACAAC
  TACAAAGTTTTTGNTTTTTGTAGCCACAAA
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_000381
• Insert Size: 3720 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000381.1, NP_000372.1
• RefSeq Size: 3452 bp
• RefSeq ORF: 2004 bp
• Locus ID: 4281
• Cytogenetics: Xp22.2
• Domains: zf-B_box, RING, BBC, SPRY, FN3
• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: 'The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Alternative promoter use, alternative splicing and alternative polyadenylation result in multiple transcript variants that have different tissue specificities. [provided by RefSeq, Dec 2016]'
  Transcript Variant: This variant (1, also known as alpha) is the longest transcript. Variants 1-4 and variant 10 encode the same isoform (1).