MID1 (NM_033291) Human Untagged Clone

CAT#: SC109439

MID1 (untagged)-Human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MID1
• Synonyms: OS, FXY, OSX, OGS1, XPRF, BBBG1, GBBB1, RNF59, ZNFXY, TRIM18
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_033291, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAAACACTGGAGTCAGAACTGACCTGCCCTATTTGTCTGGAGCTCTTTGAGGACCCTCTTCTACTGC
  CCTGCGCACACAGCCTCTGCTTCAACTGCGCCCACCGCATCCTAGTATCACACTGTGCCACCAACGAGTC
  TGTGGAGTCCATCACCGCCTTCCAGTGCCCCACCTGCCGGCATGTCATCACCCTCAGCCAGCGAGGTCTA
  GACGGGCTCAAGCGCAACGTCACCCTACAGAACATCATCGACAGGTTCCAGAAAGCATCAGTGAGCGGGC
  CCAACTCTCCCAGCGAGACCCGTCGGGAGCGGGCCTTTGACGCCAACACCATGACCTCCGCCGAGAAGGT
  CCTCTGCCAGTTTTGTGACCAGGATCCTGCCCAGGACGCTGTGAAGACCTGTGTCACTTGTGAAGTATCC
  TACTGTGACGAGTGCCTGAAAGCCACTCACCCGAATAAGAAGCCCTTTACAGGCCATCGTCTGATTGAGC
  CAATTCCGGACTCTCACATCCGGGGGCTGATGTGCTTGGAGCATGAGGATGAGAAGGTGAATATGTACTG
  TGTGACCGATGACCAGTTAATCTGTGCCTTGTGTAAACTGGTTGGGCGGCACCGCGATCATCAGGTGGCA
  GCTTTGAGTGAGCGCTATGACAAATTGAAGCAAAACTTAGAGAGTAACCTCACCAACCTTATTAAGAGGA
  ACACAGAACTGGAGACCCTTTTGGCTAAACTCATCCAAACCTGTCAACATGTTGAAGTCAATGCATCACG
  TCAAGAAGCCAAATTGACAGAGGAGTGTGATCTTCTCATTGAGATCATTCAGCAAAGACGACAGATTATT
  GGAACCAAGATCAAAGAAGGGAAGGTGATGAGGCTTCGCAAACTGGCTCAGCAGATTGCAAACTGCAAAC
  AGTGCATTGAGCGGTCAGCATCACTCATCTCCCAAGCGGAACACTCTCTGAAGGAGAATGATCATGCGCG
  TTTCCTACAGACTGCTAAGAATATCACCGAGAGAGTCTCCATGGCAACTGCATCCTCCCAGGTTCTAATT
  CCTGAAATCAACCTCAATGACACATTTGACACCTTTGCCTTAGATTTTTCCCGAGAGAAGAAACTGCTAG
  AATGTCTGGATTACCTTACAGCTCCCAACCCTCCCACAATTAGAGAAGAGCTCTGCACAGCTTCATATGA
  CACCATCACTGTGCATTGGACCTCCGATGATGAGTTCAGCGTGGTCTCCTACGAGCTCCAGTACACCATA
  TTCACCGGACAAGCCAACGTCGTTAGTCTGTGTAATTCGGCTGATAGCTGGATGATAGTACCCAACATCA
  AGCAGAACCACTACACGGTGCACGGTCTGCAGAGCGGCACCAAGTACATCTTCATGGTCAAGGCCATCAA
  CCAGGCGGGCAGCCGCAGCAGTGAGCCTGGGAAGTTGAAGACAAACAGCCAACCATTTAAACTGGATCCC
  AAATCTGCTCATCGAAAACTGAAGGTGTCCCATGATAACTTGACAGTAGAACGTGATGAGTCATCATCCA
  AGAAGAGTCACACACCTGAACGCTTCACCAGCCAGGGGAGCTATGGAGTAGCTGGAAATGTGTTTATTGA
  TAGTGGCCGGCATTATTGGGAAGTGGTCATAAGTGGAAGCACATGGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_033291 unedited
  NCCTGTCGGATTTTGTATACGACTCCTATAGGCGGCNCGCGAATTCGCACGAGGCTCGAG
  CAGAGTGCGTGTAGCAACAGATCAAAGAAAAAGAGACGAAAACGTGCTCTTTGCTGCCCG
  TAGATTTCGCCGGGTTGCTTTTGTCTTGCGGGCTCCTGTCGGGTTCGGTGTTTCCGCTCT
  GAAGACTGCGACGCGGGCTCCGATGCAGCTCGCTCCCTGCCGGATGGGTCATGGGATTCT
  AAACATGAGGCAGATAGCTGATCAGCTTCCTTGGGTTTTGCTGATGACACAAGAGAGCTT
  TGCCTGAAGATGGAAACACTGGAGTCAGAACTGACCTGCCCTATTTGTCTGGAGCTCTTT
  GAGGACCCTCTTCTACTGCCCTGCGCACACAGCCTCTGCTTCAACTGCGCCCACCGCATC
  CTAGTATCACACTGTGCCACCAACGAGTCTGTGGAGTCCATCACCGCCTTCCAGTGCCCC
  ACCTGCCGGCATGTCATCACCCTCAGCCAGCGAGGTCTAGACGGGCTCAAGCGCAACGTC
  ACCCTACAGAACATCATCGACAGGTTCCAGAAAGCATCAGTGAGCGGGCCCAACTCTCCC
  AGCGAGACCCGTCGGGAGCGGGCCTTTGACGCCAACACCATGACCTCCGCCGAGAAGGTC
  CTCTGCCAGTTTTGTGACCAGGATCCTGCCCAGGACGCTGTGAAGACCTGTGTCACTTGT
  GAAGTATCCTACTGTGACGAGTGCCTGAAAGCCACTCACCCGAATAAGAAGCCCTTTACA
  GGCCATCGTCTGATTGAGCCAATTCCGGACTCTCACATNNCGNNGGCTGATGTGCTTGGA
  GCATGANNGATGAGAAGTGGAAATATGTACTGTGTGACCGATGACCAGTTAATCTGTGCC
  CTGTGTAAACTGGNNTGGCGNCACCGCGATCATCAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_033291 unedited
  ACTATGAACCGCGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTGGTCTTA
  CAACTCTATTGTAAACTATACTAGACTATAGAGGGACTTCTACATCTTTCAAGATGTGTT
  TAATAAAGGTCTGTTTATAATAACTTTTGAGGCATGAATCTAGCAAATAGTACTTTATAC
  AATGTCCCTTGTCATTACCAACTCATAAATATTAAGTGTTTTTCAGTGACTTATGTTTGG
  ATGTGGTAGTGCTGATCAGGGCCATGTGCTGATGTCCTGGAGAGCAAAATCAATCCAAAG
  TGGTGCTGCTATTTGTGACAGAACATGTTTATTTACTCAGCCCCGGAGACAAAAGGAAAA
  TTGATATGGGGGAGCGGGAAATAGGAGAACTATTAAATGTAGTGAAGAAATTTCACAGGT
  CTAAAGGAACTATTAAAAGGAAGGATAAAGTAGATTCTATACTATAAAACAGAATCCTAC
  CTCTGATAAAAGACAAATCAGCCTGAATTTTTGAATAATCAATAGGATTCAAAATGACTA
  TTTTCAATTGCAATCTCATTCTTAATGTTCTTCCAGAACCCTTAACCCTGAGAGAAGCAG
  GGCAAAATGTGGCCAAAGGATTCAGAACCTTTCTTTCCAACATTATCCCATCAGCTATGA
  GAAGGTAAAGATTTAGCCACGGCAAGGTCCGAGGTCCAAAATCAACTCTTTTCATTCTCT
  GTCTTGCTCTCCATTTTAGTTGATCTGATACAACTTTTTGACATCACATTAAAACACTTC
  AATTATACCCNAGTAAGAAGACTGTTTTCTNCCCTACTAAGTTCAACACATCCTGTTATG
  CCCACTCGAGTGAGAAGACTACCCTGGGTCATCATGGGTATCAGGTAGAGATGAGTACAT
  CCAACTCCAAATTTTGTTTTTTGTAGCCCCGATGTGATAAATAAAGTGTAATA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_033291
• Insert Size: 3600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033291.1, NP_150633.1
• RefSeq Size: 3352 bp
• RefSeq ORF: 1659 bp
• Locus ID: 4281
• Cytogenetics: Xp22.2
• Domains: zf-B_box, RING, BBC, FN3
• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: 'The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Alternative promoter use, alternative splicing and alternative polyadenylation result in multiple transcript variants that have different tissue specificities. [provided by RefSeq, Dec 2016]'
  Transcript Variant: Variant 2 (beta) uses an alternate splice site near the end of the coding region, introducing a frameshift and an earlier stop codon as compared to variant 1. The beta isoform is thus 115 aa shorter than isoform alpha.