RFC3 (NM_002915) Human Untagged Clone

CAT#: SC109722

RFC3 (untagged)-Human replication factor C (activator 1) 3, 38kDa (RFC3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RFC3
• Synonyms: RFC38
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109722 sequence for NM_002915 edited (data generated by NextGen Sequencing)
  ATGAGCCTCTGGGTGGACAAGTATCGGCCCTGCTCCTTGGGACGGCTGGACTATCACAAG
  GAGCAGGCGGCCCAGCTGCGGAACCTGGTGCAGTGTGGTGACTTTCCTCATCTGTTAGTG
  TACGGACCATCAGGTGCTGGAAAAAAGACAAGAATTATGTGTATTCTACGTGAACTTTAT
  GGTGTTGGAGTGGAAAAATTGAGAATTGAACATCAGACCATCACAACTCCATCTAAAAAA
  AAAATTGAAATTAGCACCATTGCAAGTAACTACCACCTTGAAGTTAATCCTAGTGATGCT
  GGAAATAGTGACCGAGTAGTCATTCAGGAGATGTTGAAAACAGTGGCACAATCACAACAA
  CTTGAAACAAACTCTCAAAGGGATTTTAAAGTGGTATTATTGACAGAAGTTGACAAACTC
  ACCAAAGATGCTCAGCATGCCTTGCGAAGAACCATGGAAAAATATATGTCTACCTGCAGA
  TTGATCTTGTGCTGCAATTCTACATCTAAAGTGATCCCACCTATTCGTAGTAGGTGCTTG
  GCGGTTCGTGTGCCTGCTCCCAGCATTGAAGATATTTGCCACGTGTTATCTACTGTGTGT
  AAGAAGGAAGGTCTGAATCTTCCTTCACAACTGGCTCATAGACTTGCAGAGAAGTCTTGT
  AGAAATCTCAGAAAAGCCCTGCTTATGTGTGAAGCCTGCAGAGTGCAACAATATCCTTTT
  ACTGCAGATCAAGAAATCCCTGAGACAGATTGGGAGGTGTATCTGAGGGAGACTGCAAAT
  GCTATTGTCAGTCAGCAAACTCCACAAAGGCTCCTTGAAGTTCGTGGAAGGCTGTATGAG
  CTTCTAACTCATTGTATTCCTCCTGAGATAATAATGAAGGGCCTTCTCTCAGAACTGTTA
  CATAATTGTGATGGACAACTGAAAGGGGAGGTGGCACAAATGGCAGCTTACTATGAGCAT
  CGTCTACAGCTGGGTAGCAAAGCCATTTATCACTTGGAAGCGTTTGTGGCCAAATTCATG
  GCACTTTATAAGAAGTTCATGGAGGATGGATTGGAAGGCATGATGTTCTGA
  
  Clone variation with respect to NM_002915.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002915 unedited
  NGCTTGTCAAAATTTGTATACGACTCACTATAGGCGGCCGCGAATCGGCACGAGGAGGAC
  GCGCGCTCGCGCGGNGATTTCAAGCGTAGGCCCCCGGGAACTCGAGCTGCCATGAGCCTC
  TGGGTGGACAAGTATCGGCCCTGCTCCTTGGGACGGCTGGACTATCACAAGGAGCAGGCG
  GCCCAGCTGCGGAACCTGGTGCAGTGTGGTGACTTTCCTCATCTGTTAGTGTACGGACCA
  TCAGGTGCTGGAAAAAAGACAAGAATTATGTGTATTCTACGTGAACTTTATGGTGTTGGA
  GTGGAAAAATTGAGAATTGAACATCAGACCATCACAACTCCATCTAAAAAAAAAATTGAA
  ATTAGCACCATTGCAAGTAACTACCACCTTGAAGTTAATCCTAGTGATGCTGGAAATAGT
  GACCGAGTAGTCATTCAGGAGATGTTGAAAACAGTGGCACAATCACAACAACTTGAAACA
  AACTCTCAAAGGGATTTTAAAGTGGTATTATTGACAGAAGTTGACAAACTCACCAAAGAT
  GCTCAGCATGCCTTGCGAAGAACCATGGAAAAATATATGTCTACCTGCAGATTGATCTTG
  TGCTGCAATTCTACATCTAAAGTGATCCCACCTATTCGTAGTAGGTGCTTGGCGGTTCGT
  GTGCCTGCTCCCAGCATTGAAGATATTTGCCACGTGTTATCTACTGTGTGTAAGAAGGAA
  GGTCTGAAATCTTCTTCACAACTGGNCTCATAGACTGCAGAGAAGTCTTGTAGAAATCTC
  AGAAAGCCCTGCTTATGTGTGAAGCCTGCAGAGTGCACAATATCCTTTTACTGCAGATCA
  GNAAATCCCCTGAGACAGATGGGAGGTGTATCTGTAGGAGACTGCAAATGCTATTGTCAG
  TCAGCAAACTCCACAAAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002915 unedited
  GCGGCACGCAATCTAAAATCGAGTTTTTTTTTTTTTTTTTTTAAGTATTCACAGCAACTT
  TATTCATAATAACCAAACCTGGAAACAAACCAAAGATCCATCAGCAAGTGAATAGGCTTT
  AAAAGTATATACAAAAATATTGAACAATAAACATTAACTAGTGAAACATTCAACAATATG
  GATGAATCTCAATAATGAGAGAGAATGAAGTATTACACAAGAATGCTTACAATATCTGAT
  TCCATTAAATTCTTGAACTGGCAAAACTCATCTATAATGACAAGATCAGTGATCATCTGG
  GCCAGGGTAGGGATGAACTGACTGCAAAGGGATATGGAGGGAACTTTTTGGGGTGACGGC
  ATCTATACCTTGATTGGGATCATAGTTTCACTGGTCAAAAATCAAACTGTACCCTTAAAA
  TGGGTATATTTTATTTTATAAATTATACTTCGTAGTAAAGGAATTGTTCGGAGTCTGTCA
  CACAAATTTGTCAAGTAAACCCTTTGGTCCATGAACCAAAATATTTAATTAAAAAGGGCA
  GAGCTAATGACAAAACACAAAACCTGTTCAAAGAGTAATTACTACAGTAACATACCTTTC
  TCCAGAACTTCATTCTGAATTTTCTTCAAGAGTCTCAAGTACTTAAATTAGTGAAAACAT
  GCTTATTTCTTAGTACCGACCTAATACTAATCTGAACCACTGAAATAAAAGTTGGAAATC
  TGCAGCCTCAACAGAAATGGTATCTGCAATTTTGTCCATACCAATAATTATGTTTCAGAA
  GGAAATCTTACATTTCCTACAAAAATACATTATTCATTGAGAGAAACTAGAATTAATATA
  TGGTGTGTCAACAATCATGNTACCTGTGGCAGAAAATGGCATCTGGATAATAAAATAATC
  TTGCTTCTATGNGNAATCTCTTTACAGTGAGAGATTACGGAGAATTNGAGGATCACATGA
  TACTGATAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002915
• Insert Size: 2380 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002915.2, NP_002906.1
• RefSeq Size: 2380 bp
• RefSeq ORF: 1071 bp
• Locus ID: 5983
• Cytogenetics: 13q13.2
• Domains: AAA
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: DNA replication, Mismatch repair, Nucleotide excision repair
• Gene Summary: 'The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longer isoform (1).