UBE3B (NM_183415) Human Untagged Clone

CAT#: SC110718

UBE3B (untagged)-Human ubiquitin protein ligase E3B (UBE3B), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UBE3B
• Synonyms: BPIDS; KOS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_183415, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTTCACCCTGTCTCAGACCTCGAGAGCATGGTTCATCGATAGAGCCCGTCAGGCACGAGAAGAAAGGC
  TTGTGCAGAAGGAACGGGAGCGGGCAGCTGTTGTGATCCAGGCCCATGTCCGGAGTTTTCTCTGTCGGAG
  TCGACTGCAGAGAGATATCAGGAGAGAGATTGATGACTTTTTTAAAGCAGATGACCCTGAGTCCACTAAA
  AGAAGTGCACTTTGTATTTTCAAGATTGCCAGGAAACTGCTGTTCCTATTCAGAATCAAAGAGGATAATG
  AGAGATTTGAGAAGTTGTGTCGCAGCATCCTGAGCAGCATGGATGCTGAGAATGAGCCTAAGGTGTGGTA
  TGTGTCCCTGGCTTGTTCTAAGGACCTCACCCTCCTTTGGATTCAACAGATCAAGAACATTTTGTGGTAC
  TGCTGTGATTTTCTCAAGCAGCTCAAGCCTGAAATCCTGCAGGACTCCCGACTCATCACCCTGTACCTCA
  CGATGCTTGTCACCTTCACAGACACTTCAACGTGGAAAATTCTTCGGGGAAAAGGTGAAAGTCTTCGACC
  AGCGATGAACCACATTTGTGCAAATATAATGGGACATCTCAACCAGCATGGATTTTATTCTGTGCTGCAG
  ATATTGTTAACCCGTGGCCTGGCAAGACCCCGTCCTTGTCTATCCAAAGGCACTTTAACAGCAGCTTTTT
  CTCTAGCGTTACGCCCTGTGATTGCTGCACAGTTCTCAGACAATCTGATTCGGCCGTTCCTCATCCACAT
  CATGTCTGTGCCTGCTCTGGTGACTCATCTCAGCACAGTGACCCCTGAGCGCCTCACTGTTTTAGAATCC
  CATGACATGCTTCGTAAATTCATCATATTTTTAAGAGACCAAGATCGATGCCGTGATGTATGTGAAAGTT
  TAGAAGGATGCCATACGCTTTGTCTAATGGGCAACCTCCTACACTTGGGCTCCCTCAGCCCCAGAGTGTT
  AGAGGAGGAGACAGATGGGTTCGTGAGTTTGCTCACCCAGACGCTGTGCTACTGTCGGAAGTATGTGTCT
  CAGAAGAAGTCCAACCTGACCCACTGGCATCCTGTCCTTGGCTGGTTCTCCCAATCTGTGGATTATGGCC
  TTAACGAGTCAATGCACTTGATCACCAAACAGCTGCAGTTCTTGTGGGGGGTGCCTCTGATCCGGATCTT
  CTTCTGTGACATCCTGAGCAAGAAGCTACTGGAGAGCCAGGAGCCAGCCCACGCACAGCCAGCATCCCCT
  CAGAATGTGCTCCCAGTGAAGAGTCTCCTAAAGCGTGCTTTTCAAAAGTCGGCATCAGTCCGGAATATTC
  TCAGGCCTGTCGGGGGTAAACGGGTCGACTCTGCAGAAGTCCAGAAGGTTTGCAACATCTGTGTCCTCTA
  CCAGACCTCGCTGACAACTCTCACACAGATTCGGCTGCAGATACTCACAGGTCTCACTTACCTTGATGAC
  CTGCTTCCCAAACTGTGGGCATTTATCTGTGAGCTCGGGCCCCACGGAGGGTTAAAGCTCTTCTTGGAAT
  GCCTGAACAATGACACTGAAGAGTCCAAGCAACTCTTGGCCATGCTGATGCTGTTCTGTGACTGTTCGCG
  GCACCTCATCACAATCCTTGATGACATTGAAGTTTATGAAGAACAGATTTCATTCAAACTGGAAGAGCTG
  GTCACTATCTCCTCTTTCCTGAATTCTTTTGTGTTTAAGATGATCTGGGATGGAATTGTAGAGAACGCCA
  AGGGTGAGACCTTGGAGCTGTTCCAGTCTGTCCACGGGTGGCTTATGGTGCTGTACGAGCGGGACTGCCG
  GCGGCGCTTCACCCCCGAGGACCACTGGCTGCGAAAGGATCTCAAACCTAGCGTGCTCTTCCAAGAACTC
  GACAGGGACAGAAAACGGGCACAGTTGATCCTGCAGTACATCCCACATGTCATCCCTCACAAAAACAGAG
  TTCTACTGTTTCGAACCATGGTTACCAAGGAGAAGGAGAAACTGGGGCTGGTGGAAACCAGCTCTGCCTC
  CCCGCATGTCACTCACATCACCATCCGCCGGTCCAGGATGCTGGAGGACGGCTACGAGCAGCTTAGGCAG
  CTCTCCCAGCACGCCATGAAGGGGGTCATCCGTGTGAAGTTTGTCAATGACCTCGGGGTGGACGAAGCAG
  GGATTGATCAAGACGGTGTTTTTAAGGAGTTCTTGGAAGAGATCATCAAGAGAGTTTTTGACCCAGCACT
  CAATCTGTTCAAGACAACCAGTGGGGATGAGAGGCTGTACCCCTCACCCACATCCTACATCCATGAGAAT
  TACCTGCAGCTCTTCGAGTTTGTGGGGAAGATGCTGGGGAAGGCTGTGTATGAGGGAATTGTGGTGGACG
  TGCCATTTGCATCCTTCTTCCTGAGCCAACTGCTTGGGCACCACCACAGCGTCTTCTATAGCTCGGTGGA
  TGAACTGCCTTCTCTGGACTCCGAGTTCTATAAAAACCTCACCTCCATCAAGCGCTATGATGGGGACATC
  ACTGACCTGGGCCTGACGCTGTCTTACGACGAGGACGTCATGGGTCAGCTTGTTTGCCATGAACTGATTC
  CTGGAGGGAAGACCATTCCTGTTACAAATGAAAATAAAATTAGCTACATCCATCTGATGGCACATTTTCG
  AATGCACACTCAAATAAAAAACCAAACAGCTGCCCTCATTAGCGGATTCCGTTCCATTATCAAACCCGAG
  TGGATCCGAATGTTCTCAACTCCTGAACTGCAGCGTCTCATCTCTGGCGACAATGCTGAGATTGATCTGG
  AAGATTTAAAGAAGCACACAGTCTACTACGGTGGTTTCCATGGAAGTCACAGAGTCATCATCTGGCTCTG
  GGATATTCTGGCCTCCGACTTCACACCGGATGAGAGAGCTATGTTTCTGAAGTTCGTGACCAGCTGCTCC
  AGACCCCCGCTCCTGGGATTCGCCTACCTCAAGCCTCCATTCTCCATCCGCTGCGTGGAGGTGTCGGACG
  ATCAGGACACCGGGGACACTCTGGGCAGCGTCCTCCGGGGCTTCTTCACCATCCGCAAGCGGGAGCCAGG
  CGGCCGCCTGCCCACCTCCTCCACCTGCTTCAACCTGCTCAAGCTGCCCAACTACAGCAAGAAGAGCGTC
  CTCCGCGAGAAGCTGCGCTACGCCATCAGCATGAACACGGGCTTTGAACTCTCCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_183415 unedited
  TTGTATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGTAGTGCGTCGGCT
  GCTGCCCCGGGTCTGGCAGAACTCGGGTGTTTTGGGCTGAGACAGTGGCAGCTGCGGCCC
  CGACCCCAAGTGCGGGGACCTCCGGCGAATAAAGAAAAAATAACAAGCTTTTCTGAAGTG
  AGAAGCTGTTCTCAGCCACGAGTCCTGTGCAAGATCACTAATGATTACCTGGCATTTCTG
  CGACACAGGCAGGTCCTCAGGGTTTGTGCAAGTTTGCAAACATGTTCACCCTGTCTCAGA
  CCTCGAGAGCATGGTTCATCGATAGAGCCCGTCAGGCACGAGAAGAAAGGCTTGTGCAGA
  AGGAACGGGAGCGGGCAGCTGTTGTGATCCAGGCCCATGTCCGGAGTTTTCTCTGTCGGA
  GTCGACTGCAGAGAGATATCAGGAGAGAGATTGATGACTTTTTTAAAGCAGATGACCCTG
  AGTCCACTAAAAGAAGTGCACTTTGTATTTTCAAGATTGCCAGGAAACTGCTGTTCCTAT
  TCAGAATCAAAGAGGATAATGAGAGATTNGAGAAGTTGTGTCGCAGCATCCTGAGCAGCA
  TGGATGCTGAGAATGAGCCTAAGGGTGTGGTNATGTGTCCCTGGGCTTGTTCTAAGGACC
  TCACCCTCCTTTGGATTCAACAGATAAGACATTTTTTGGGGGTACTGCTGGGGATTTTCT
  CAAGCAGCTCAAGCCTGAAATCCTGCAGGACTCCCGACTCATCACCCTGTACCTACGGAT
  GCTTGTCACCTTCACAGACCTTCACGGTGGGAAATTCTTCGGGGAAAGGGGGGGAAAGTT
  CTCGACCAGCGATGAACCACATTTGTGCAATATTAATGGGACATCTCACAGCTTGGATTT
  TTTTATTCTGGCTGGAAAAATTGTAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_183415 unedited
  GTACTATGGACCGCGCGCCGCTTTCTANGATCGANATTTTTTTTTTTTTTTTTTAATATG
  CTGAATTACAAATTTAATAAAGCCTTTGATCACATCTCAATCCATAAGTTAGCTACAAAA
  ACAAAAAATCCTACAACTTTTTAGAGCCATCAAGAGCCATCAAGGTGTCAGTGAACACCA
  GTTACAAAAGGAGATTCAGTGTTTCAGATAGTGCAATATTTCCAGCTGCTGAAAAGAATT
  TAAATGAAATTACAAACTACCCCTCCTTGTCAAAAATCCACATGAAGTTGATATTGGTGC
  TTATAAATCACTCTCTCCCAGTCCCTCACTGGTTCCAACCTTCAGGTGATAAAAATTAGG
  ATGGGATCCATCTTCCCTGTGCTGACAGTCTGGGGTCCCCGCATGTATGCACGAACCCGC
  CCAGCGTGCGCACACACGTTCAGAAGAAATCTTCAAAGGAACCGAGCGTTTGGAGAAAGT
  GGCAAGTCCACAGAATCAGAGGTTACGAACACACCTTCAATAATATTAATACATTCCTGT
  CTTTAAATTCCTTGCCATGTTTCCATCAAAGTAGAGCACACACTCTTTTCCAGAAGCCTG
  GGGGCTCGACCTGGGTGGGACACCAGGATGCAGCTGAAACTCTGAAGGACTGTGGAGACG
  GGTGGCCCCTGCTTTGCTGCCACTGTGACAGCTCTTGTTGGAAAATGTTTTGCGTCTTTG
  AGGAGAGCAGAGGAGGGACTGGGCCTTCCTGGCTTCGAATCCGGCATCAACCTTGGTGGT
  GCAGGTGCAGGAGCATGAGGTGGAGAGAGGNGGNTTNCCTNNCAAACCNACCACAAACGC
  GGCTCCGCCTCACCCCCTGGAGCTAAGTTCTTACCACCAAGCCCAGCAGAGGTTATCAGA
  CCCATTGGTTGGAACACATGCACCTTACAAAACCAACACCCACG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_183415
• ORF Size: 3207 bp
• Insert Size: 6050
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_183415.1, NP_904324.1
• RefSeq Size: 5404
• RefSeq ORF: 3207
• Locus ID: 89910
• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin-protein ligases. This gene encodes a member of the E3 ubiquitin-conjugating enzyme family which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme and transfers the ubiquitin to the targeted substrates. A HECT (homology to E6-AP C-terminus) domain in the C-terminus of the longer isoform of this protein is the catalytic site of ubiquitin transfer and forms a complex with E2 conjugases. Shorter isoforms of this protein which lack the C-terminal HECT domain are therefore unlikely to bind E2 enzymes. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2012]
  Transcript Variant: This variant (3) uses an alternate splice site in the 5' UTR, as compared to variant 1. Variants 1 and 3 encode the same protein (isoform 1).