TCPTP (PTPN2) (NM_002828) Human Untagged Clone

CAT#: SC111683

PTPN2 (untagged)-Human protein tyrosine phosphatase, non-receptor type 2 (PTPN2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PTPN2
• Synonyms: PTN2; PTPT; TC-PTP; TCELLPTP; TCPTP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002828, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCCACCACCATCGAGCGGGAGTTCGAAGAGTTGGATACTCAGCGTCGCTGGCAGCCGCTGTACTTGG
  AAATTCGAAATGAGTCCCATGACTATCCTCATAGAGTGGCCAAGTTTCCAGAAAACAGAAATCGAAACAG
  ATACAGAGATGTAAGCCCATATGATCACAGTCGTGTTAAACTGCAAAATGCTGAGAATGATTATATTAAT
  GCCAGTTTAGTTGACATAGAAGAGGCACAAAGGAGTTACATCTTAACACAGGGTCCACTTCCTAACACAT
  GCTGCCATTTCTGGCTTATGGTTTGGCAGCAGAAGACCAAAGCAGTTGTCATGCTGAACCGCATTGTGGA
  GAAAGAATCGGTTAAATGTGCACAGTACTGGCCAACAGATGACCAAGAGATGCTGTTTAAAGAAACAGGA
  TTCAGTGTGAAGCTCTTGTCAGAAGATGTGAAGTCGTATTATACAGTACATCTACTACAATTAGAAAATA
  TCAATAGTGGTGAAACCAGAACAATATCTCACTTTCATTATACTACCTGGCCAGATTTTGGAGTCCCTGA
  ATCACCAGCTTCATTTCTCAATTTCTTGTTTAAAGTGAGAGAATCTGGCTCCTTGAACCCTGACCATGGG
  CCTGCGGTGATCCACTGTAGTGCAGGCATTGGGCGCTCTGGCACCTTCTCTCTGGTAGACACTTGTCTTG
  TTTTGATGGAAAAAGGAGATGATATTAACATAAAACAAGTGTTACTGAACATGAGAAAATACCGAATGGG
  TCTTATTCAGACCCCAGATCAACTGAGATTCTCATACATGGCTATAATAGAAGGAGCAAAATGTATAAAG
  GGAGATTCTAGTATACAGAAACGATGGAAAGAACTTTCTAAGGAAGACTTATCTCCTGCCTTTGATCATT
  CACCAAACAAAATAATGACTGAAAAATACAATGGGAACAGAATAGGTCTAGAAGAAGAAAAACTGACAGG
  TGACCGATGTACAGGACTTTCCTCTAAAATGCAAGATACAATGGAGGAGAACAGTGAGAGTGCTCTACGG
  AAACGTATTCGAGAGGACAGAAAGGCCACCACAGCTCAGAAGGTGCAGCAGATGAAACAGAGGCTAAATG
  AGAATGAACGAAAAAGAAAAAGGTGGTTATATTGGCAACCTATTCTCACTAAGATGGGGTTTATGTCAGT
  CATTTTGGTTGGCGCTTTTGTTGGCTGGACACTGTTTTTTCAGCAAAATGCCCTATAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002828 unedited
  TCAGCATTTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGCCCGGATCGT
  GCGGGGCCTGAGCCTCTCCGCCGGCGCAGGCTCTGCTCGCGCCAGCTCGCTCCCGCAGCC
  ATGCCCACCACCATCGAGCGGGAGTTCGAAGAGTTGGATACTCAGCGTCGCTGGCAGCCG
  CTGTACTTGGAAATTCGAAATGAGTCCCATGACTATCCTCATAGAGTGGCCAAGTTTCCA
  GAAAACAGAAATCGAAACAGATACAGAGATGTAAGCCCATATGATCACAGTCGTGTTAAA
  CTGCAAAATGCTGAGAATGATTATATTAATGCCAGTTTAGTTGACATAGAAGAGGCACAA
  AGGAGTTACATCTTAACACAGGGTCCACTTCCTAACACATGCTGCCATTTCTGGCTTATG
  GTTTGGCAGCAGAAGACCAAAGCAGTTGTCATGCTGAACCGCATTGTGGAGAAAGAATCG
  GTTAAATGTGCACAGTACTGGCCAACAGATGACCAAGAGATGCTGTTTAAAGAAACAGGA
  TTCAGTGTGAAGCTCTTGTCAGAAGATGTGAAGTCGTATTATACAGTACATCTACTACAA
  TTAGAAAATATCAATAGTGGTGAAACCAGAACAATATCTCACTTTCATTATACTACCTGG
  CCAGATTTTGGAGTCCCTGAATCACCAGCTTCATTTCTCAATTTCTTGTTTAAAGTGAGA
  GAATCTGGCTCCTTGAACCCTGACATGGGCCTGCGGTGATCCACTGTAGTGCAGGCATTG
  NGCGCTCTGGCACCTTCTCTCTGGAGACACTTGTCTTGTTTTGATGGAAAAGGAGATGAT
  TTTAACTAAAACAGTGTTACTGAACTGAGAAATACGAATGGGCTTATTCGACCCAGATCA
  CTGAGATCTCATACTGGCTTAAAGAAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002828 unedited
  ACTATGGCCGCGGCCGCATCTAGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTAACCAGTTTTAAACAAACATGAATGTTTTTTTTTTAA
  ACAGCCTTTTACAGTTGGGGGTTCAAAGGAACCTGCAGCCAAAAACCCTGAAATGTGGGG
  CTTGGGACTGAAAATATCACTTATCGGGGGGAGGTCTATTCTACGGCCCCGTTTTTGGAA
  TATGAGGCGTTTGTTGCAAACAAACCCCTATGATTAAGGGACCACTGGCAGTTACTAGGG
  CAAAAGCTTGCTGGGCAAAATTAATTGTTTATAGGGCATTTTGCTGAAAAAACAGGGTCC
  ACCCAACAAAAGCCCCACCCAAAAGGACTGACATAACCCCCATTTTAGGGAAAATAGGTT
  GCCAATATACCCACCTTTTTTTTTTTCGTTAATTCTAATTAACCCTCTGTTTCATCGGCT
  GCACCTTTTGAGCTGGGGGGGCCTTTTTGCCCTTTCGAAAACGTTTCCGAAAAGCACTCC
  CACTGTTCTCCTCCATTGGATCTGGCATTATAAAGGAAAGCCCTGTACATCGGCCACCTG
  CCACTTTTTTTTCTTCTAAACCTATTTTGCGCCCATTGATTTTTTAACCCATTTTTTTGT
  TGGGCGAAAGATAAAAGGCAGGAAACAAGCTCTTCTTAAAAAGCCTTTCCATCGTTCCGG
  ACAACTAAAAACCCCTTTATAACTTTGGCCCTTTTATTTAACGCCTTGGTGAAAACCCCA
  GTGATCTGGGGTCAGAAAAAACCCCATTCGGTTTTTTCACTGTTAAGACCCACTGGGTCA
  AGGCAAACACATCCCCTTTTCCCCACAACAAGAATAGTTCTCCCCAAAAAAAGGTCAAAA
  CCCCCAATCCTCGCTTACATGGGACCCCCAGCGCCCCGCGTTGGGTTCAGGCACACAATT
  CTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002828
• Insert Size: 1700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002828.2, NP_002819.1
• RefSeq Size: 2477 bp
• RefSeq ORF: 1248 bp
• Locus ID: 5771
• Cytogenetics: 18p11.21
• Domains: Y_phosphatase, PTPc_motif
• Protein Families: Druggable Genome, Phosphatase, Transmembrane
• Gene Summary: 'The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. Multiple alternatively spliced transcript variants encoding different isoforms have been found. Two highly related but distinctly processed pseudogenes that localize to chromosomes 1 and 13, respectively, have been reported. [provided by RefSeq, May 2011]'
  Transcript Variant: This variant (1) encodes the longest isoform (1).