GLS2 (NM_013267) Human Untagged Clone

CAT#: SC111793

GLS2 (untagged)-Human glutaminase 2 (liver, mitochondrial) (GLS2), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GLS2
• Synonyms: GA; GLS; hLGA; LGA
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111793 sequence for NM_013267 edited (data generated by NextGen Sequencing)
  ATGCGCTCCATGAAGGCTCTGCAGAAGGCCCTGAGCCGGGCTGGCAGTCACTGCGGGCGA
  GGAGGCTGGGGTCACCCGAGCCGGAGCCCCCTCCTTGGCGGGGGCGTCCGGCACCACCTC
  AGTGAGGCCGCGGCGCAGGGCAGAGAGACGCCACACAGCCACCAGCCGCAGCACCAGGAT
  CATGATTCATCAGAAAGTGGCATGCTGTCCCGCCTGGGTGATTTGCTCTTTTACACTATT
  GCTGAAGGACAGGAACGAATCCCTATCCACAAGTTCACCACTGCACTAAAGGCCACTGGA
  CTGCAGACATCAGATCCTCGGCTCCGAGACTGCATGAGCGAGATGCACCACGTGGTCCAA
  GAGTCCAGTAGTGGTGGCCTCTTGGACCGAGATCTCTTCCGAAAGTGTGTGAGCAGCAAC
  ATTGTGCTCCTGACCCAGGCATTCCGAAAGAAGTTTGTCATTCCTGATTTTGAGGAGTTC
  ACGGGCCATGTGGATCGCATCTTTGAGGATGTCAAAGAGCTCACTGGAGGCAAAGTGGCA
  GCCTACATCCCTCAGCTGGCCAAGTCAAACCCAGACCTGTGGGGTGTCTCCCTGTGCACT
  GTGGATGGTCAACGGCACTCTGTGGGCCACACAAAGATCCCCTTCTGCCTGCAGTCCTGT
  GTGAAGCCCCTCACCTATGCCATCTCCATAAGCACCCTAGGCACTGACTACGTGCACAAG
  TTTGTGGGCAAAGAGCCAAGTGGCCTGCGCTACAACAAGCTCTCCCTCAATGAGGAAGGA
  ATCCCCCATAACCCCATGGTCAATGCTGGTGCCATTGTTGTCAGCTCCCTGATCAAGATG
  GACTGTAACAAAGCAGAGAAGTTTGATTTTGTGTTGCAGTATCTCAACAAAATGGCTGGG
  AATGAATACATGGGTTTCAGCAATGCCACATTCCAGTCAGAGAAGGAAACAGGGGATCGG
  AATTATGCCATCGGCTATTATCTCAAGGAAAAGAAGTGCTTTCCTAAGGGGGTGGACATG
  ATGGCTGCCCTTGATCTCTACTTCCAGCTGTGTTCTGTGGAGGTCACTTGTGAATCAGGC
  AGTGTCATGGCAGCCACCCTCGCCAACGGTGGGATCTGCCCCATCACAGGCGAGAGTGTG
  CTGAGTGCTGAAGCAGTGCGCAACACCCTCAGCCTCATGCATTCCTGCGGCATGTATGAC
  TTCTCTGGCCAGTTTGCCTTCCACGTGGGCCTGCCAGCCAAGTCAGCTGTATCAGGAGCC
  ATCCTCCTGGTGGTACCCAATGTCATGGGAATGATGTGCCTGTCACCCCCATTGGACAAG
  CTGGGGAACAGCCATAGGGGGACCAGCTTCTGCCAGAAGTTGGTGTCTCTCTTCAATTTC
  CACAACTATGACAACCTGAGGCACTGTGCTCGGAAGTTAGACCCACGGCGTGAAGGGGCA
  GAAATTCGGAACAAGACTGTGGTCAACCTGTTATTTGCTGCCTATAGTGGCGATGTCTCA
  GCTCTTCGAAGGTTTGCCTTGTCAGCCATGGATATGGAACAGAAAGACTATGACTCGCGC
  ACAGCTCTGCATGTTGCTGCAGCTGAAGGACACATCGAAGTTGTTAAATTCCTGATCGAG
  GCTTGCAAAGTGAATCCTTTTGCCAAGGACAGGTGGGGCAACATTCCCCTGGATGATGCT
  GTGCAGTTCAACCATCTGGAGGTGGTCAAACTGCTTCAAGATTACCAGGACTCCTACACA
  CTCTCTGAAACTCAGGCTGAGGCAGCAGCTGAGGCCCTGTCCAAAGAGAACTTAGAAAGC
  ATGGTATGA
  
  Clone variation with respect to NM_013267.2
  350 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013267 unedited
  CCCCCGTTCAAAATTGTAAACGACTCACTATAGGCGGCCTGCGAATCGGCACGAGGCTTG
  CTGGGGCATGCGCTCCATGAAGGCTCTGCAGAAGGCCCTGAGCCGGGCTGGCAGTCACTG
  CGGGCGAGGAGGCTGGGGTCACCCGAGCCGGAGCCCCCTCCTTGGCGGGGGCGTCCGGCA
  CCACCTCAGTGAGGCCGCGGCGCAGGGCAGAGAGACGCCACACAGCCACCAGCCGCAGCA
  CCAGGATCATGATTCATCAGAAAGTGGCATGCTGTCCCGCCTGGGTGATTTGCTCTTTTA
  CACTATTGCTGAAGGACAGGAACGAATCCCTATCCACAAGTTCACCACTGCACTAAAGGC
  CACTGGACTGCAGACATCAGATCCTCGGCTCCGAGACTGCATGAGCGAGATGCACCACGT
  GGTCCAAGAGTCCAGTAGTGGTGGCCTCTTGGACCGAGATCTCTTCCGAAAGTGTGTGAG
  CAGCAACATTGTGCTCCTGACCCAGGCATTCCGAAAGAAGTTTGTCATTCCTGATTTTGA
  GGAGTTCACGGGCCATGTGGATCGCATCTTTGAGGATGTCAAAGAGCTCACTGGAGGCAA
  AGTGGCAGCCTACATCCCTCAGCTGGCCAAGTCAAACCCAGACCTGTGGGGTGTCTCCCT
  GTGCACTGTGGATGGTCAACGGCACTCTGTGGGCCACACAAAGATCCCCTTCTGCCTGCA
  GTCCTGTGTGAAGCCCCTCACCTATGCCATCTNCATAAGCACCCTAGGCACTGACTACGT
  GCACAAGTTTGTGGGCACAGAGCCAAGTGGCCTGCGCTACAAACACGCTCTCCTCATGAG
  GCAGGAATTCCCCATAACCCATGGTCAAGCTGGTGCCATTGTTGTCAGCTCCTGATCAGA
  TGGACTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_013267 unedited
  TTTTTTTTTTTTTTTTTGATTTGGTAATTAAAGTTTATTTGAACACAAAATACTTNTCTC
  TGTCTATAAAATTGGCTGTTAGCAGTAGCAGCAGCATGTCCTGGCCAAGGGGAGTAGATT
  TCTCCAGACTACTAAAGCCATGTATATAGCCATTCCCACTTCCCATATTCTGTGGATATG
  TACATGTGCATGGTAGCTAGAGTCCCTCCACCAGAGTATCTCTCTCATGATGGTTTCTTC
  AGGGAGAGGAAGAGGAGACCTGGGTGAAGAGCTGGGATGGTTTTGAGTGGGGCAAGCCAT
  TAGGCTGTACCTTGAAGN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_013267
• ORF Size: 1809 bp
• Insert Size: 2440
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_013267.2, NP_037399.2
• RefSeq Size: 2636
• RefSeq ORF: 1809
• Locus ID: 27165
• Domains: ANK, Glutaminase
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, D-Glutamine and D-glutamate metabolism, Metabolic pathways, Nitrogen metabolism
• Gene Summary: The protein encoded by this gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Originally thought to be liver-specific, this protein has been found in other tissues as well. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (1) encodes the longest isoform (1).