TRIM5 alpha (TRIM5) (NM_033034) Human Untagged Clone

CAT#: SC111855

TRIM5 (untagged)-Human tripartite motif containing 5 (TRIM5), transcript variant alpha

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TRIM5
• Synonyms: RNF88; TRIM5alpha
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_033034 edited
  GAATTCGGCACCAGCCAGGACAGGCAGGAGCAGTGGAATAGCTAGTATGGCTTCTGGAAT
  CCTGGTTAATGTAAAGGAGGAGGTGACCTGCCCCATCTGCCTGGAACTCCTGACACAACC
  CCTGAGCCTGGACTGCGGCCACAGCTTCTGCCAAGCATGCCTCACTGCAAACTACAAGAA
  GTCCATGCTAGACAAAGGAGAGAGTAGCTGCCCTGTGTGCCGGATCAGTTACCAGCCTGA
  GAACATACGGCCTAATCGGCATGTAGCCAACATAGTGGAGAAGCTCAGGGAGGTCAAGTT
  GAGCCCAGAGGGGCAGAAAGTTGATCATTGTGCACGCCATGGAGAGAAACTTCTACTCTT
  CTGTCAGGAGGACGGGAAGGTCATTTGCTGGCTTTGTGAGCGGTCTCAGGAGCACCGTGG
  TCACCACACGTTCCTCACAGAGGAGGTTGCCCGGGAGTACCAAGTGAAGCTCCAGGCAGC
  TCTGGAGATGCTGAGGCAGAAGCAGCAGGAAGCTGAAGAGTTAGAAGCTGACATCAGAGA
  AGAGAAAGCTTCCTGGAAGACTCAAATACAGTATGACAAAACCAACGTCTTGGCAGATTT
  TGAGCAACTGAGAGACATCCTGGACTGGGAGGAGAGCAATGAGCTGCAAAACCTGGAGAA
  GGAGGAGGAAGACATTCTGAAAAGCCTTACGAACTCTGAAACTGAGATGGTGCAGCAGAC
  CCAGTCCCTGAGAGAGCTCATCTCAGATCTGGAGCATCGGCTGCAGGGGTCAGTGATGGA
  GCTGCTTCAGGGTGTGGATGGCGTCATAAAAAGAGCTGACAGATGTCCGACGCTACTGGG
  TTGATGTGACAGTGGCTCCAAACAACATTTCATGTGCTGTCATTTCTGAAGATAAGAGAC
  AAGTGAGCTCTCCGAAACCACAGATAATATATGGGGCACGAGGGACAAGATACCAGACAT
  TTGTGAATTTCAATTATTGTACTGGCATCCTGGGCTCTCAAAGTATCACATCAGGGAAAC
  ATTACTGGGAGGTAGACGTGTCCAAGAAAACTGCTTGGATCCTGGGGGTATGTGCTGGCT
  TCCAACCTGATGCAATGTGTAATATTGAAAAAAATGAAAATTATCAACCTAAATACGGCT
  ACTGGGTTATAGGGTTAGAGGAAGGAGTTAAATGTAGTGCTTTCCAGGATAGTTCCTTCC
  ATACTCCTTCTGTTCCTTTCATTGTGCCCCTCTCTGTGATTATTTGTCCTGATCGTGTTG
  GAGTTTTCCTAGACTATGAGGCTTGCACTGTCTCATTCTTCAATATCACAAACCATGGAT
  TTCTCATCTATAAGTTTTCTCACTGTTCTTTTTCTCAGCCTGTATTTCCATATTTAAATC
  CTAGAAAATGTGGAGTCCCCATGACTCTGTGCTCACCAAGCTCTTGAACCTTCTTACACA
  CTCAGCCCCTTCTGTACAGCACCTCTTGTCCAGGTGCATCTCATACACCTGAACTCATTT
  GCATCATTTTAACCATCTTTTCCTTGCTGTCTCCCTTCTTTCTATTTGAACGTCCTTCAC
  TCATCAGTAAAATGTAATAATTGCCTTGTGCCATATTGTCCCCAATATTTTATTGACATT
  TGATAGCAATTTTTTTCATCATTTTCCGTACTCCTAAGGAAAACTGACCTATACCTCATA
  AAATGAGACCGCTATTTAGGTATTACTTCTGCCAGATATTTATCACCCAATTGCCTCTGA
  CACTGACTAAGAAGATGAAGAAAAGCTTTTCAACAGCCTTTCTATATCATCGTGTGATAA
  TTGTTCACCAATGAATGAGTCCTTAGCCCTGTGTCAGTTTACCCTCGATGCCCTTATTTG
  TGAGTTAAAGAGAAAATATCATAAATGGTATACTCTTAAGTATAGAGGTTTTGTATCTAG
  AGGATCTCAGTTCAACTCCTGTCTCTCCATATACCAGCAGTGTAACTGTGAATAACATAC
  TTAAATGGCTGTGCTTATTTCCTTTTCTTTTCTTTTTTCTTTTTTTTTTTTTTTGAGATG
  AAGTTTTGCTCTTGTTCCCCAGGCTGGAGTGCAATGGCACGATCTCGGTTCACTGCAACC
  TCCACCTCTCAGATTCAAGCAATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAG
  GTGCCCACCACCACCCCTGGCTAAATTTGTATTTTCAGTAGAGACGGGGTTTCCCCATGT
  TGGTTAGGCTCGTCTAGAACCTCTGACCTCAGGTGATCCACCCGCCTCGGCCTCCCAAAG
  TGCTGGGATTACAGGCGTGAGCCACGGCGCCCAGCCTGTGCTTATTTTCTTAAAATAATT
  TTTGTATTAAAAACTTCACATTAAATAAGTGCTAATGTTTTATTGCATAGTAGGGTGACT
  AGAGTTAACAATAACCTATTGCATATATTTTGAAATAGCTAGAAGAGAGGATTTTGAAAG
  TTCTCAACACAAAGAAATGACACATATTTGAGGTGATGGATATGCTAATTACCCTGGTTC
  GGTTATTACGCAATGTATACATGTATCAAAACATCACACTGTACCACATAAATATGTATA
  TTTATTATTTGTCAATTAAAAGCAAAATAAAACAAAAAACCTTCATCTAATACTTTGGAT
  CATTGTGAAAAAATAAATTCCTGAAGTATAAAGCATCTXXXXXXXXXXXXXXXXAACTCG
  AC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_033034 unedited
  CAAATTTATGTATACGACTCACTATAGGGCGGCCGCGNAATTCGCACCAGCCAGGACAGG
  CAGGAGCAGTGGAATAGCTAGTATGGCTTCTGGAATCCTGGTTAATGTAAAGGAGGAGGT
  GACCTGCCCCATCTGCCTGGAACTCCTGACACAACCCCTGAGCCTGGACTGCGGCCACAG
  CTTCTGCCAAGCATGCCTCACTGCAAACTACAAGAAGTCCATGCTAGACAAAGGAGAGAG
  TAGCTGCCCTGTGTGCCGGATCAGTTACCAGCCTGAGAACATACGGCCTAATCGGCATGT
  AGCCAACATAGTGGAGAAGCTCAGGGAGGTCAAGTTGAGCCCAGAGGGGCAGAAAGTTGA
  TCATTGTGCACGCCATGGAGAGAAACTTCTACTCTTCTGTCAGGAGGACGGGAAGGTCAT
  TTGCTGGCTTTGTGAGCGGTCTCAGGAGCACCGTGGTCACCACACGTTCCTCACAGAGGA
  GGTTGCCCGGGAGTACCAAGTGAAGCTCCAGGCAGCTCTGGAGATGCTGAGGCAGAAGCA
  GCAGGAAGCTGAAGAGTTAGAAGCTGACATCAGAGAAGAGAAAGCTTCCTGGAAGACTCA
  AATACAGTATGACAAAACCAACGTCTTGGCAGATTNTGAGCAACTGAGAGACATCCTGGA
  CTGNGAGGAGAGCAATGAGCTGCANAACCTGNAGAAAGAGGANGAAGACATTCTGAAAAG
  CCTTACGAACTCTGAAACTGAGATGGTGCAGCAGACCCAGTCCCTGAGAGAGCTCATCTC
  AGATCTGGAGCATCGGCTGCAGGGGTCAGTGATGGAGCTGCCTCAAGGTGTTGGATTGCG
  TCATAAAAAGAGCTGACAGATGTCCGACGCTACTNGGGTTGATGTGACAGTGGCTCCAAA
  CAACATTAATGGGCTGCCATTCCTGAGAATAGAGAAAGGGAGCCTTCGGAACCCAGTAAA
  TATATGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_033034 unedited
  TTGGACCGCGGCCGCAATCTACAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTAAAAGGCTTAAAACTTCGAGGAATTTATTTTTTACCAAGGATCCC
  AAAGTTTTAAAGGAGGGGTTTTGGTTTAATTTGGCTTTAAATGGCCAAAAAAAAAATATC
  CCTATTTATGGGGCCCAGGGGGAGGTTTTGAAACATGTTTACTTTGGGAAAAACCCGACC
  CAGGGGAATAACCAAACCCTTTTTTTTAAAAAGGGGCCATTTTTTTGGGGGGAAAACTTT
  AAAAATCCTTTTTTTTAGCTATTCCAAAATATATGCAAAAGGTTATTGTTAACCTTAGCC
  CCCCTCCTTTGCAAAAAAACATTCCCCCTTATTTAAGGGGAAGCTTTTAAATCAAAAATT
  ATTTTAAAAAAAAAACCCCCGGCTGGGCCCCGGGGCTCCCCCCTGAAACCCCACACTTGG
  GGAGGCCCAGGCGGGGGGACCCCCTGGGGTCACAGGCTCAACACCACCCCCACACACATG
  GGGCACACCCCCCCTTCCAATGAACCACCAACCCCAACCACCGGGTGGGGGGGGGCCCCT
  CCACACCCCCCCCTGGCGCGGCCCGCCCCGAACACCCTTCTCTCCCTCCACCCGAGCGGC
  CACACCACCCCACAACCTCCCCCCTCCCCCCACACCGGCCCCATCCACCCACCATCTTTC
  TCTCTCCCCCTCAATCCCAACCCCTCCTTTACGCCCACCCCCCTCTCCTCCCCCCCTTCC
  TCCCCCCCCACCCCTTCCCCTATCTCACCACCCCCCGCTAACAACTCCCTCACTCACACT
  CCCCCCCCCCCCCCCCAAACCACCCTCCCCCCCCCCCCCCCCCCAAAACCCAATATCCTC
  CCCCCTCCCCCCCCATACCCATTCCCCCACCCCCCCCACCTTACTCTACCCCACTTCCAC
  CCCCCACTCCCTCCCCCCACCCCCCCACCCCCTCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_033034
• ORF Size: 1482 bp
• Insert Size: 2750
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_033034.1, NP_149023.1
• RefSeq Size: 2990
• RefSeq ORF: 1482
• Locus ID: 85363
• Domains: zf-B_box, RING, SPRY
• Protein Families: Druggable Genome
• Gene Summary: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein forms homo-oligomers via the coilel-coil region and localizes to cytoplasmic bodies. It appears to function as a E3 ubiquitin-ligase and ubiqutinates itself to regulate its subcellular localization. It may play a role in retroviral restriction. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2009]
  Transcript Variant: This variant (alpha) encodes the longest isoform (alpha). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.