ABAT (NM_000663) Human Untagged Clone

CAT#: SC111922

ABAT (untagged)-Human 4-aminobutyrate aminotransferase (ABAT), nuclear gene encoding mitochondrial protein, transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ABAT
• Synonyms: GABA-AT; GABAT; NPD009
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111922 sequence for NM_000663 edited (data generated by NextGen Sequencing)
  ATGGCCTCCATGTTGCTCGCCCAGCGCCTGGCCTGCAGCTTCCAGCACAGCTACCGCCTG
  CTGGTGCCTGGATCCAGACACATTAGTCAAGCTGCAGCCAAAGTCGACGTTGAATTTGAT
  TATGATGGGCCTCTGATGAAGACGGAAGTCCCAGGGCCTAGATCTCAGGAGTTAATGAAA
  CAGCTGAATATAATTCAGAATGCAGAGGCTGTGCATTTTTTCTGCAATTACGAAGAGAGC
  CGAGGCAATTACCTGGTTGATGTGGACGGCAACCGAATGCTGGATCTTTATTCCCAGATC
  TCCTCTGTTCCCATAGGTTACAGCCACCCCGCCCTGCTGAAACTCATCCAACAGCCTCAA
  AATGCGAGCATGTTTGTCAACAGACCCGCCCTCGGAATCCTGCCTCCGGAGAACTTTGTG
  GAGAAGCTCCGGCAGTCCTTGCTCTCGGTGGCTCCCAAAGGGATGTCCCAGCTCATCACC
  ATGGCCTGCGGCTCCTGCTCCAATGAAAACGCCTTAAAGACCATCTTCATGTGGTACCGG
  AGCAAGGAAAGAGGGCAGAGGGGCTTCTCCCAGGAGGAGCTGGAGACGTGCATGATTAAC
  CAGGCCCCTGGCTGCCCCGACTACAGCATCCTCTCCTTCATGGGCGCGTTCCATGGGAGG
  ACCATGGGTTGCTTAGCGACCACGCACTCTAAAGCCATTCACAAGATCGACATCCCTTCC
  TTTGACTGGCCCATCGCACCGTTCCCACGGCTGAAATACCCTCTGGAAGAGTTTGTGAAA
  GAGAACCAACAGGAGGAGGCCCGCTGTCTGGAAGAGGTGGAGGATCTGATTGTGAAATAT
  CGGAAAAAGAAGAAGACGGTGGCCGGGATCATCGTGGAGCCCATCCAGTCCGAGGGTGGA
  GACAACCACGCATCCGATGACTTCTTTCGGAAGCTGAGAGACATCGCCAGGAAGCATGGC
  TGCGCCTTCTTGGTGGACGAGGTCCAGACCGGAGGAGGCTGCACGGGCAAGTTCTGGGCC
  CATGAGCACTGGGGCCTGGATGACCCAGCAGACGTGATGACCTTCAGCAAGAAGATGATG
  ACTGGGGGCTTCTTCCACAAGGAGGAGTTCAGGCCTAATGCTCCCTACCGGATCTTCAAC
  ACCTGGCTGGGGGACCCGTCCAAGAACCTGTTGCTGGCTGAGGTCATCAACATCATCAAG
  CGGGAGGACCTGCTAAATAATGCAGCCCATGCCGGGAAGGCCCTGCTCACAGGACTGCTG
  GACCTCCAGGCCCGGTACCCCCAGTTCATCAGCAGGGTGAGAGGACGAGGCACCTTTTGC
  TCCTTCGATACTCCCGATGATTCCATACGGAATAAGCTCATTTTAATTGCCAGAAACAAA
  GGTGTGGTGTTGGGTGGCTGTGGTGACAAATCCATTCGTTTCCGTCCCACGCTGGTCTTC
  AGGGATCACCACGCTCACCTGTTCCTCAATATTTTCAGTGACATCTTAGCAGACTTCAAG
  TAA
  
  Clone variation with respect to NM_000663.4
  309 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000663 unedited
  GTTCACATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGGCGAGTCGCT
  GGGGGAAGTCGAGCAGACACCCAGCGCTGCCGGAACTCGGGGCGCGCATCCCGCAAGTCG
  GAACCCGGCGGATCGGATCCCGCAGATCGGAGACGGGGCCTGGAGATGCGCCCATCCAGG
  GTCTAGCGGATTGCAAAGGGCCTGGTAGAATCAAAGAGGGATATACTAATGCAACTTCGA
  GGGTGGCAGCACGCAAAGGGTGTCCCTGTCCCTCAAGGGGTCATGGCCTCCATGTTGCTC
  GCCCAGCGCCTGGCCTGCAGCTTCCAGCACAGCTACCGCCTGCTGGTGCCTGGATCCAGA
  CACATTAGTCAAGCTGCAGCCAAAGTCGACGTTGAATTTGATTATGATGGGCCTCTGATG
  AAGACGGAAGTCCCAGGGCCTAGATCTCAGGAGTTAATGAAACAGCTGAATATAATTCAG
  AATGCAGAGGCTGTGCATTTTTTCTGCAATTACGAAGAGAGCCGAGGCAATTACCTGGTT
  GATGTGGACGGCAACCGAATGCTGGATCTTTATTCCCAGATCTCCTCTGTTCCCATAGGT
  TACAGCCACCCCGCCCTGCTGAAACTCATCCAACAGCCTCAAAATGCGAGCATGTTTGTC
  AACAGACCCGCCCTCGGAATCCTGCCTCCGGAGAACTTTGTGGAGAAGCTCCGGCAGTCC
  TTGCTCTCGGTGGCTCCCNAGGGATGTCCCAGCTCATCACCATGGCCTGCGGCTCCTGCT
  CCAATGANACGCCTTNAAGACATCTTCATGTGGTACCGGAGCAGGAAAGAGGNCAGAGGG
  GCTTCTCCCAGGAGAGCTGGAGACGTGCATGATTAACCANGCCCCTGGCTGCCCNGATAC
  AGCATCCTCTTCTTATGGNGCGCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000663 unedited
  TGGTTTTTTTACGNATTCTTACTAACACAGGNAAACAGCATTTAATTCATGGGAATTGTG
  CATATGGTCATCCGTTACATTGTGACATGTTAATTTTTTTTTTATCATTTATTGGCACTG
  TCAACAGATTACTTGTGAACAAGATCACTTTGTACGCTTAAGTCTGCGATGCTACTTAGC
  TATGGTTTTCTACCATGAGCTTATATATAGATAGGTGTAGGTATGTAGATACATTAATGC
  TATACACAATTTTGCATGGTTACTGAGCGTCAGTAAAAATTATGAAAAAACACCCATTTA
  TAATAAAAGTGAGGATGTACTAAGACTTGCTATTACTGGACCTTGTTTTCTGTAAAAGTG
  ATGACACTTGCTGGACGGTTACTAAACTCTATGGCACTAATGTATGATGGATTCATTTCC
  AGACTGTCGGCCACGGAAGCACTTCTTCATGGCCTCTGCCCTGGACAGCAGCCTGTCCTC
  CGGGCTCCCCATGTTTTTACCAGCTTCTGCTGAGTTTCTACAATCTTGAGCTCTGCTGAG
  AATTCTTTTCCTTGAAATTCTTCTAGCTAAAGCCCCAGCCCCCAAAGGAGCATGTCTCAG
  GAACTCATTATGCCCTGAGTCAACAAGAACTTGTTGATAAATGGCTTAAAAGTTTTTACA
  AGAAGTAACTTCCCTTGGTAAGGAGTAAATAATAGCTCTGGAATTTTCCAGATAAAACTA
  TTTCATTTCTCTGTCAGTGCCCCATGGGGAGAGAACGAAATATTGGAGCCCCTCTCCCTA
  CCAAAGAGAGCCACTNTTCTGGTTGTGCCCTGGCTTAAACCCTTTGGACTCCGAGAACAT
  ACTGATATTTGACCCATGCTAAATTTTCAGGAGAAGCTACTTAAGCATAATGATATGGTT
  GGTTGATTTGCTGCTGCTAATAGCCTANTGACTTGGAGCTGCTGGGAACTGGCTCCAGGA
  AGAGTGAGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000663
• Insert Size: 4880 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000663.2, NP_000654.2
• RefSeq Size: 4763 bp
• RefSeq ORF: 1503 bp
• Locus ID: 18
• Cytogenetics: 16p13.2
• Domains: aminotran_3
• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, beta-Alanine metabolism, Butanoate metabolism, Metabolic pathways, Propanoate metabolism, Valine, leucine and isoleucine degradation
• Gene Summary: '4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) has an alternate 5' UTR, as compared to variant 1.