SHFM3 (FBXW4) (NM_022039) Human Untagged Clone

CAT#: SC112849

FBXW4 (untagged)-Human F-box and WD repeat domain containing 4 (FBXW4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FBXW4
• Synonyms: DAC; FBW4; FBWD4; SHFM3; SHSF3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112849 sequence for NM_022039 edited (data generated by NextGen Sequencing)
  ATGGCGGCGGCGGCCGGGGAGGAGGAGGAGGAGGAGGAGGCGGCTCGGGAGTCGGCTGCC
  CGCCCGGCCGCGGGGCCTGCGCTCTGGCGCCTGCCGGAGGAGCTGCTGCTGCTCATCTGC
  TCCTACCTGGACATGCGGGCCCTCGGCCGCCTGGCCCAGGTGTGCCGCTGGCTGCGGCGC
  TTCACCAGCTGCGATCTGCTCTGGCGCCGGATAGCCCGGGCCTCGCTCAACTCCGGCTTC
  ACGCGGCTCGGCACCGACCTGATGACCAGTGTCCCAGTGAAGGAACGAGTGAAGGTGTCT
  CAGAACTGGAGACTGGGGCGCTGCCGAGAGGGGATTCTGCTGAAGTGGAGATGCAGTCAG
  ATGCCCTGGATGCAGCTAGAGGATGATTCTCTGTACATATCCCAGGCTAATTTCATCCTG
  GCCTACCAGTTCCGTCCAGATGGTGCCAGCTTGAATCGTCGGCCTCTGGGAGTCTTTGCT
  GGGCATGATGAGGACGTTTGCCACTTTGTGCTGGCCAACTCGCATATTGTTAGTGCAGGA
  GGGGATGGGAAGATTGGCATTCATAAGATTCACAGCACCTTCACTGTCAAGTACTCGGCT
  CATGAACAGGAGGTGAACTGTGTGGATTGCAAAGGGGGCATCATTGTGAGTGGCTCCAGG
  GACAGGACGGCCAAGGTGTGGCCTTTGGCCTCAGGCCGGCTGGGGCAGTGCTTACACACC
  ATCCAGACTGAAGACCGAGTCTGGTCCATTGCTATCAGCCCATTACTCAGCTCTTTTGTG
  ACAGGGACGGCTTGTTGCGGGCACTTCTCACCCCTGAGAATCTGGGACCTCAACAGTGGG
  CAGCTGATGACACACTTGGGCAGTGACTTTCCCCCAGGGGCTGGGGTGCTGGATGTCATG
  TATGAGTCCCCTTTCACACTGCTGTCCTGTGGCTATGACACCTATGTTCGCTACTGGGAC
  CTCCGCACCAGCGTCCGGAAATGTGTCATGGAGTGGGAGGAGCCCCACGACAGCACCCTG
  TACTGCCTGCAGACAGATGGCAACCACCTGCTGGCCACAGGTTCCTCCTACTACGGTGTT
  GTACGGCTGTGGGACCGGCGTCAAAGGGCCTGCCTGCACGCCTTCCCGCTGACGTCGACT
  CCCCTCAGCAGCCCTGTGTACTGCCTGCGTCTCACCACCAAGCATCTCTATGCTGCCCTG
  TCTTACAACCTCCACGTCCTGGATTTTCAAAACCCATGA
  
  Clone variation with respect to NM_022039.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022039 unedited
  GGGCACGATTAGGTAATAGCGACTCCTATAGGGGCGGCCGCGAATTCGCACGAGGGGGAC
  AGGGGTGGCCATGGCGGCGGCGGCCGGGGAGGAGGAGGAGGAGGAGGAGGCGGCTCGGGA
  GTCGGCTGCCCGCCCGGCCGCGGGGCCTGCGCTCTGGCGCCTGCCGGAGGAGCTGCTGCT
  GCTCATCTGCTCCTACCTGGACATGCGGGCCCTCGGCCGCCTGGCCCAGGTGTGCCGCTG
  GCTGCGGCGCTTCACCAGCTGCGATCTGCTCTGGCGCCGGATAGCCCGGGCCTCGCTCAA
  CTCCGGCTTCACGCGGCTCGGCACCGACCTGATGACCAGTGTCCCAGTGAAGGAACGAGT
  GAAGGTGTCTCAGAACTGGAGACTGGGGCGCTGCCGAGAGGGGATTCTGCTGAAGTGGAG
  ATGCAGTCAGATGCCCTGGATGCAGCTAGAGGATGATTCTCTGTACATATCCCAGGCTAA
  TTTCATCCTGGCCTACCAGTTCCGTCCAGATGGTGCCAGCTTGAATCGTCGGCCTCTGGG
  AGTCTTTGCTGGGCATGATGAGGACGTTTGCCACTTTGTGCTGGCCAACTCGCATATTGT
  TAGTGCAGGAGGGGATGGGAAGATTGGCATTCATAAGATTCACAGCACCTTCACTGTCAA
  GTACTCGGCTCATGAACAGGAGGTGAACTGTGTGGATTGCAAAGGGGGCATCATTGTGAG
  TGGCTCCAGGGACAGGACGGCCAAGGTGTGGCCTTTGGCCTCANGCCGGCTGGNGCAGTG
  CTTCACACCATCCAGACTGAAGACCGAGTCTGGTCCATTGCTATCAGCCCATTACTCAGC
  TCTTTTGTGACAGGGACGGCTTGTTGCGGGCACTTCTCACCCCTGGAAATCTGGGACCTC
  ACAGTGGGCAGCTGATGACCCACTTGGCAGTGACTTCCCCCAGGGCTGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_022039 unedited
  CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTGAACAATCAAGCTTTA
  TTTTTACAAAAATGAAAATTGTAGCATGTCTCAACAGCCAGCCTGAGGTAGGGCTGGCTC
  AAAACTCACTCAGAACTGCTGGGTTCCCTTTGCTCTTTCTTGGTTTCAAAGCAAAACCAG
  GCCACAGTGCCCCAGGAATGAACCCCCAGCCCCGAGAGGTTGGTCAAGGGTGAGGTCTGG
  CTGGTGCCAGGAGGCTATTGGCAGCCTCACCTTTCCTTCCAGGAAGGGGCAGAAAGGAAC
  CATCTGGGAAGGTGGGAGCACCTCACCTCACTCTGTAGGCTGTCATGGCTCTAATAAGGG
  GTATAGGATGGGCCTCTCCAGGCCAGATCCTTGCTCTGGCCAAACAGGGACGCAAGGCCC
  GGGTTCAGCCGCACTCTAAAGCAGCAGGTCCTGCCTCTCCAACAGGTTCCTGGGAGGGAC
  TGCATCTCTGGTAAGCCTCAAAGTCCCAGGAGTCAGAAGCCTCTAGTGCAGGTGCCTGAG
  CACTGGGGTCACAGGCCCAGGAGCACAGCGGGGCAGTGAGGAGGAGCTATCACTGCCCCC
  TCCACGCAACACAAGCCCCTGAGTCGTTGGCTTCCCTGGCCACGAGGCCAGGGTGGCCCT
  GACGGCCATGGTTTTGCAAACCCAGAACGGGAGGCCGTTGACAGGGCACCCCATAAATGT
  TGGGTGGCGAGAACCCGCATCCACACGGGTTGCTGAAGGCAGTCGACCACATCGGGAAGC
  GTGCAGGACGGCCCTTTGACGCGGCCCCCACCGGACAATACCGACATGGAGAACCCGGGC
  GCAATGGGGGTCCCCCCTGTTTGAGAAATCACGGCGCTGTCTGGGGGTCTCCCACCCCCG
  AACCCCCCGGCCCTGCGGGGGGGTCCCCATCGAACATAAGTCCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_022039
• Insert Size: 1950 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022039.3, NP_071322.1
• RefSeq Size: 2303 bp
• RefSeq ORF: 1239 bp
• Locus ID: 6468
• Cytogenetics: 10q24.32
• Domains: WD40, F-box
• Protein Families: Druggable Genome
• Gene Summary: 'This gene is a member of the F-box/WD-40 gene family, which recruit specific target proteins through their WD-40 protein-protein binding domains for ubiquitin mediated degradation. In mouse, a highly similar protein is thought to be responsible for maintaining the apical ectodermal ridge of developing limb buds; disruption of the mouse gene results in the absence of central digits, underdeveloped or absent metacarpal/metatarsal bones and syndactyly. This phenotype is remarkably similar to split hand-split foot malformation in humans, a clinically heterogeneous condition with a variety of modes of transmission. An autosomal recessive form has been mapped to the chromosomal region where this gene is located, and complex rearrangements involving duplications of this gene and others have been associated with the condition. A pseudogene of this locus has been mapped to one of the introns of the BCR gene on chromosome 22. [provided by RefSeq, Jul 2008]'