VPS35 (NM_018206) Human Untagged Clone

CAT#: SC113645

VPS35 (untagged)-Human vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VPS35
• Synonyms: MEM3; PARK17
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113645 sequence for NM_018206 edited (data generated by NextGen Sequencing)
  ATGCCTACAACACAGCAGTCCCCTCAGGATGAGCAGGAAAAGCTCTTGGATGAAGCCATA
  CAGGCTGTGAAGGTCCAGTCATTCCAAATGAAGAGATGCCTGGACAAAAACAAGCTTATG
  GATGCTCTAAAACATGCTTCTAATATGCTTGGTGAACTCCGGACTTCTATGTTATCACCA
  AAGAGTTACTATGAACTTTATATGGCCATTTCTGATGAACTGCACTACTTGGAGGTCTAC
  CTGACAGATGAGTTTGCTAAAGGAAGGAAAGTGGCAGATCTCTACGAACTTGTACAGTAT
  GCTGGAAACATTATCCCAAGGCTTTACCTTTTGATCACAGTTGGAGTTGTATATGTCAAG
  TCATTTCCTCAGTCCAGGAAGGATATTTTGAAAGATTTGGTAGAAATGTGCCGTGGTGTG
  CAACATCCCTTGAGGGGTCTGTTTCTTCGAAATTACCTTCTTCAGTGTACCAGAAATATC
  TTACCTGATGAAGGAGAGCCAACAGATGAAGAAACAACTGGTGACATCAGTGATTCCATG
  GATTTTGTACTGCTCAACTTTGCAGAAATGAACAAGCTCTGGGTGCGAATGCAGCATCAG
  GGACATAGCCGAGATAGAGAAAAAAGAGAACGAGAAAGACAAGAACTGAGAATTTTAGTG
  GGAACAAATTTGGTGCGCCTCAGTCAGTTGGAAGGTGTAAATGTGGAACGTTACAAACAG
  ATTGTTTTGACTGGCATATTGGAGCAAGTTGTAAACTGTAGGGATGCTTTGGCTCAAGAA
  TATCTCATGGAGTGTATTATTCAGGTTTTCCCTGATGAATTTCACCTCCAGACTTTGAAT
  CCTTTTCTTCGGGCCTGTGCTGAGTTACACCAGAATGTAAATGTGAAGAACATAATCATT
  GCTTTAATTGATAGATTAGCTTTATTTGCTCACCGTGAAGATGGACCTGGAATCCCAGCG
  GATATTAAACTTTTTGATATATTTTCACAGCAGGTGGCTACAGTGATACAGTCTAGACAA
  GACATGCCTTCAGAGGATGTTGTATCTTTACAAGTCTCTCTGATTAATCTTGCCATGAAA
  TGTTACCCTGATCGTGTGGACTATGTTGATAAAGTTCTAGAAACAACAGTGGAGATATTC
  AATAAGCTCAACCTTGAACATATTGCTACCAGTAGTGCAGTTTCAAAGGAACTCACCAGA
  CTTTTGAAAATACCAGTTGACACTTACAACAATATTTTAACAGTCTTGAAATTAAAACAT
  TTTCACCCACTCTTTGAGTACTTTGACTACGAGTCCAGAAAGAGCATGAGTTGTTATGTG
  CTTAGTAATGTTCTGGATTATAACACAGAAATTGTCTCTCAAGACCAGGTGGATTCCATA
  ATGAATTTGGTATCCACGTTGATTCAAGATCAGCCAGATCAACCTGTAGAAGACCCTGAT
  CCAGAAGATTTTGCTGATGAGCAGAGCCTTGTGGGCCGCTTCATTCATCTGCTGCGCTCT
  GAGGACCCTGACCAGCAGTACTTGATTTTGAACACAGCACGAAAACATTTTGGAGCTGGT
  GGAAATCAGCGGATTCGCTTCACACTGCCACCTTTGGTATTTGCAGCTTACCAGCTGGCT
  TTTCGATATAAAGAGAATTCTAAAGTGGATGACAAATGGGAAAAGAAATGCCAGAAGATT
  TTTTCATTTGCCCACCAGACTATCAGTGCTTTGATCAAAGCAGAGCTGGCAGAATTGCCC
  TTAAGACTTTTTCTTCAAGGAGCACTAGCTGCTGGGGAAATTGGTTTTGAAAATCATGAG
  ACAGTCGCATATGAATTCATGTCCCAGGCATTTTCTCTGTATGAAGATGAAATCAGCGAT
  TCCAAAGCACAGCTAGCTGCCATCACCTTGATCATTGGCACTTTTGAAAGGATGAAGTGC
  TTCAGTGAAGAGAATCATGAACCTCTGAGGACTCAGTGTGCCCTTGCTGCATCCAAACTT
  CTAAAGAAACCTGATCAGGGCCGAGCTGTGAGCACCTGTGCACATCTCTTCTGGTCTGGC
  AGAAACACGGACAAAAATGGGGAGGAGCTTCACGGAGGCAAGAGGGTAATGGAGTGCCTA
  AAAAAAGCTCTAAAAATAGCAAATCAGTGCATGGACCCCTCTCTACAAGTGCAGCTTTTT
  ATAGAAATTCTGAACAGATATATCTATTTTTATGAAAAGGAAAATGATGCGGTAACAATT
  CAGGTTTTAAACCAGCTTATCCAAAAGATTCGAGAAGACCTCCCGAATCTTGAATCCAGT
  GAAGAAACAGAGCAGATTAACAAACATTTTCATAACACACTGGAGCATTTGCGCTTGCGG
  CGGGAATCACCAGAATCCGAGGGGCCAATTTATGAAGGTCTCATCCTTTAA
  
  Clone variation with respect to NM_018206.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018206 unedited
  TAGCATTTTGTAATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAGGGCTTGCTG
  CAGGCTCTGGGGAGTCGCCATGCCTACAACACAGCAGTCCCCTCAGGATGAGCAGGAAAA
  GCTCTTGGATGAAGCCATACAGGCTGTGAAGGTCCAGTCATTCCAAATGAAGAGATGCCC
  GGACAAAAACAAGCTTATGGATGCTCTAAAACATGCTTCTAATATGCTTGGTGAACTCCG
  GACTTCTATGTTATCACCAAAGAGTTACTATGAACTTTATATGGCCATTTCTGATGAACT
  GCACTACTTGGAGGTCTACCTGACAGATGAGTTTGCTAAAGGAAGGAAAGTGGCAGATCT
  CTACGAACTTGTACAGTATGCTGGAAACATTATCCCAAGGCTTTACCTTTTGATCACAGT
  TGGAGTTGTATATGTCAAGTCATTTCCTCAGTCCAGGAAGGATATTTTGAAAGATTTGGT
  AGAAATGTGCCGTGGTGTGCAACATCCCTTGAGGGGTCTGTTTCTTCGAAATTACCTTCT
  TCAGTGTACCAGAAATATCTTACCTGATGAAGGAGAGCCAACAGATGAAGAAACAACTGG
  TGACATCAGTGATTCCATGGATTTTGTACTGCTCAACTTTGCAGAAATGAACAAGCTCTG
  GGTGCGAATGCAGCATCAGGGACATAGCCGAGATAGAGAAAAAAGAGAACGAGAAAGACA
  AGAACTGAGAATTTTAGTGGGAACAAATTTGGTGCGCCTCAGTCAGTTGGAAGGTGTAAA
  TGTGGAACGTTACCAACAGATTGTTTTGACTGGCATATTGGAGCCAAGTGTAAACTGTAG
  GGATGCTTTGGCTCAAGATATCTCATGGAGTGTATTATTCAAGCTCTTCCTGATGATTTC
  ACCTCCAGACTTGAATNNCTTTCTTCGGGCCTGTGCTGAATACACCAAATGTAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018206 unedited
  GACGCGCCGACGCNCCCCCACTTCCTACCCNNNGGGNNGTACTGTGNNANGAAAACACGC
  NTTCTTAGGANCGNGNTATTATCCTTTTTTTTTTCACGGATCAAGGNATTTTAATTGCAG
  ATCAGTCATGCTACTTGGGGTGATCAGCAAGCACCTTGAACACTCTACCAAGCTGAATAA
  TTTTATTAAGGTCCTGAAGGTGAGTGTCCGGAGGTGCTGGNGTAAAACACATCACAGGTA
  AGAAATGGGAAAACCTACCTCAGCATTTCTGAAAGGCACAATCTATGGAAGGGAAACCTA
  GCGTAATAAAACCCTCACTGGATGTACATGGAAAGGAGTATGGTGAGCTATTTCCTTTTT
  AAAGGATGAGACCTTCATAAATTGGCCCCCTCGATTCTGGTGATTCCCGCCGCAAGCGCA
  AATGCTCCAGTGTGTTATGAAAATGTTTGTTAATCTGCTCTGTTTCTTCACTGGATTCAA
  GATTCGGGAGGTCTTCTCGAATCTTTTGGATAAGCTGGTTTAAAACCTGAATTGTTACCG
  CATCATTTTCCTTTTCATAAAAATAGATATATCTGTTCAGAATTTCTATAAAAAGCTGCA
  CTTGCAGAGAGGGGTCCATGCACTGATTTGCTATTTTTAGAGCTTTTTTTAGGCACTCCA
  TTACCCTCTTGCCTCCGAGAAGCTCCCTCCCCATTTCTGTCCGTGGTTCTGCCCGACCAA
  AAGAGATGTGCCCAGGGGCTCACAGCTCGGCCCTGAACAGGGTTCTTTTAAAGTTTGCAT
  GCCCCAGGGCCCCCTGAGTCCCCAAAGTTCATGAATTTTTTACCTGAACCATTTATTCCT
  TCCAAAAGGCCCATGCACCAAAGGGGAGGCACCCTCCCTGGGCCTTGGAAACCCCGGATT
  CAACTCCATACAAAAAAAAAGCCTGGGCACATGATTCCTAGCCACCGGCCCCCGGATTTT
  AAAAACCAATTCTCCCAACAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_018206
• ORF Size: 2391 bp
• Insert Size: 2680
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_018206.3, NP_060676.2
• RefSeq Size: 3246
• RefSeq ORF: 2391
• Locus ID: 55737
• Domains: Vps35
• Gene Summary: This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq, Jul 2008]