TRMT1 (TRMU) (NM_018006) Human Untagged Clone

CAT#: SC113792

TRMU (untagged)-Human tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TRMU
• Synonyms: LCAL3; MTO2; MTU1; TRMT; TRMT1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113792 sequence for NM_018006 edited (data generated by NextGen Sequencing)
  ATGCAGGCCTTGCGGCACGTCGTGTGCGCCCTGTCCGGCGGCGTGGACAGCGCCGTGGCC
  GCGCTGCTGCTGAGGCGGAGAGGTTACCAGGTGACAGGGGTGTTTATGAAGAACTGGGAC
  TCACTGGATGAACATGGGGTCTGTACTGCCGACAAAGACTGTGAAGATGCTTACAGAGTT
  TGCCAGATCTTAGACATCCCTTTCCATCAAGTGTCCTACGTAAAGGAGTATTGGAATGAT
  GTGTTCAGTGACTTTTTGAATGAGTATGAAAAAGGAAGGACTCCCAATCCTGACATAGTT
  TGCAACAAGCACATCAAATTTAGTTGCTTTTTTCATTATGCTGTGGATAATCTTGGGGCA
  GATGCCATTGCCACAGGTCACTATGCAAGAACTTCCCTGGAAGATGAAGAAGTCTTTGAG
  CAGAAGCACGTTAAGAAGCCCGAAGGGCTTTTCAGAAATCGGTTTGAAGTTAGAAATGCG
  GTAAAACTCCTCCAGGCAGCTGACAGCTTTAAAGACCAGACCTTCTTTCTCAGCCAGGTT
  TCCCAGGATGCCCTGAGGAGAACCATCTTCCCTCTGGGGGGATTAACGAAAGAGTTTGTA
  AAGAAAATCGCTGCTGAGAATAGACTTCATCATGTGCTTCAGAAGAAAGAGAGCATGGGC
  ATGTGTTTCATCGGGAAGAGGAATTTTGAACATTTCCTTCTTCAGTATCTGCAGCCTCGA
  CCTGGTCACTTTATTTCCATAGAAGACAATAAGGTTCTGGGAACACATAAAGGTTGGTTC
  CTGTATACCTTGGGCCAGAGAGCAAACATAGGTGGCCTGAGAGAGCCCTGGTACGTGGTG
  GAGAAGGACAGCGTCAAGGGTGACGTGTTTGTGGCCCCCCGGACAGACCACCCAGCCCTG
  TACAGGGACCTGCTGAGGACCAGCCGCGTGCACTGGATTGCGGAGGAGCCTCCCGCAGCA
  CTGGTCCGGGACAAGATGATGGAGTGCCACTTCCGATTCCGCCACCAGATGGCACTAGTG
  CCCTGTGTGCTGACCCTCAATCAAGATGGCACCGTGTGGGTGACAGCTGTGCAGGCTGTG
  CGTGCCCTTGCCACAGGACAGTTTGCTGTGTTCTACAAGGGGGACGAGTGCCTGGGCAGC
  GGGAAGATCCTGCGGCTGGGGCCGTCTGCCTACACGCTCCAGAAGGGCCAGCGCAGAGCT
  GGGATGGCCACTGAGAGCCCCAGTGACAGCCCAGAAGATGGTCCAGGCCTGAGTCCCTTG
  CTCTGA
  
  Clone variation with respect to NM_018006.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018006 unedited
  GATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAGGGTCTAG
  TGAGGCGGGTCCCACCCGGCCTTTCCCATCAGAGCCCGAACTCGCGGTTCAGGTTCCCTC
  TAGGACGCCCTTGGCCGAGAGGAGGCCTCCAATGGCTGGCTGGGGGGCTTAGAATTTTAT
  TTTTGGTTTACAGCGCAGAAGAAGAGCAGTGAGGCCGGGGCGCGGGTGGGGAGGGCAAGG
  CGGCGGCTCAAAAAAAAAGCCAGCCCCTCGCGGCCCGACAGAGTCTCGGCGCCCCGCGGG
  TCGGTGGAGCCTCAGGGGTGAGCGCAGGCCGGAGACAGGGGAACCCGCCGCGGGACAGGG
  GAAGGCCGGACGGAACGCGGGTAAGCTGCCGCAGCTTCCGGCCGGGCCACTAGCGCGGAC
  CCTACGGCCGGGGCGGGACTTCCGGCAAGGCGCGGAAGCGGCGGTAGCTGCAGCTGGCGA
  AGTTGGGCGACTGGCGGATGCAGGCCTTGCGGCACGTCGTGTGCGCCCTGTCCGGCGGCG
  TGGACAGCGCCGTGGCCGCGCTGCTGCTGAGGCGGAGAGGTTACCAGGTGACAGGGGTGT
  TTATGAAGAACTGGGACTCACTGGATGAACATGGNGGTCTGTACTGCCGACAAAGACTGT
  GAAGATGCTTACAGAGTTTGCCAGATCTTAGACATCCCTTTCCATCAAGTGTCCTACGTA
  AAGGAGTATTGGAATGATGTGTTCAGTGACTTTNTGAATGAGTATGAANAAGGGAGGACT
  CCCAATCCTGACATAGTNNTGCACAAGCACATCANATTTAGTTGCTTNTTTCATTATGCT
  GNGGATNAATCTGGGGCAGATGCCATTGCCACAGGTCACTATNGCAGAACTTNCCTGGNA
  GTAGAAGAAGTCTTTGAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018006 unedited
  GGACGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGTTTTTGGCCTTGAA
  AAATGTTTATTCCACGCTGTCCCGACAGCCCCCTCTGCAGGTCCCCTCGGTGTACTCTGA
  GGTGGGAAACCCTCCCTGGGGGCGGTGAAGGGGAACTCGGGCCACCCCACCAGCCAGCAG
  ATGCTCCAGCAGCCAGAGCCCCAGCCTGGAGCTGAGGCTCTTCCTGGGGCTCGCCGGGCC
  CCTGCAGGCTTTTCGGACCCTCAGCCAGCCCGGCTTCCTCTGCTTTGGGCAGCAGCAAGC
  TGGCCCTTGGGCACCTGGACTGCTCACCAGCCGCCCAGCCATGGGTCCTGCTCTCCAGGT
  TCCTTCTAGCAGATCCATCTCTGTCAGAGCAAGGGACTCAGGCCTGGACCATCTTCTGGG
  CTGTCACTGGGGCTCTCAGTGGCCATCCCAGCTCTGCGCTGGCCCTTCTGGAGCGTGTAG
  GCAGACGGCCCCAGCCGCAGGATCTTCCCGCTGCCCAGGCACTCGTCCCCCTTGTAGAAC
  ACAGCAAACTGTCCTGTGGCAAGGGCACGCACAGCCTGCACAGCTGTCACCCACACGGTG
  CCATCTTGATTGAGGGTCAGCACACAGGGCACTAGTGCCATCTGGTGGCGGAATCGGAAG
  TGGCACTCCATCATCTTGTCCCGGACCAGTGCTGCGGGAGGCTCCTCCGCAATCCAGTGC
  ACGCGGCTGGTTCTCAGCAGGTCCCTGTACAGGGCTGGGTGGTCTGTCCGGNGGGCCACA
  AACACGTNACCCTTGACGCTGTCCTTCTCCACCACGTACCAGGGCTCTCCTCAGCCACCT
  ATGTTTGCTCTCTGGCCCAGGTATACAGGAACCACCTTNAGTGTCCCACGACCTTATTGG
  CTCTATGAANTAAGTGACACGCCAGGCTGCAAAACTGAANAAGAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_018006
• ORF Size: 1266 bp
• Insert Size: 2130
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_018006.3, NP_060476.2
• RefSeq Size: 1970
• RefSeq ORF: 1266
• Locus ID: 55687
• Domains: tRNA_Me_trans
• Gene Summary: This nuclear gene encodes a mitochondrial tRNA-modifying enzyme. The encoded protein catalyzes the 2-thiolation of uridine on the wobble positions of tRNA(Lys), tRNA(Glu), and tRNA(Gln), resulting in the formation of 5-taurinomethyl-2-thiouridine moieties. Mutations in this gene may cause transient infantile liver failure. Polymorphisms in this gene may also influence the severity of deafness caused by mitochondrial 12S ribosomal RNA mutations. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).