AMPK gamma 3 (PRKAG3) (NM_017431) Human Untagged Clone

CAT#: SC114107

PRKAG3 (untagged)-Human protein kinase, AMP-activated, gamma 3 non-catalytic subunit (PRKAG3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRKAG3
• Synonyms: AMPKG3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_017431 edited
  GGCGGCCGCGAATTCGGCACGAGGCCACAGTGTCGCACACTTGCTCTCAGTTGGTCTGGG
  GCTGGCCACATGGAGCCCGGGCTGGAGCACGCACTGCGCAGGACCCCTTCCTGGAGCAGC
  CTTGGGGGTTCTGAGCATCAAGAGATGAGCTTCCTAGAGCAAGAAAACAGCAGCTCATGG
  CCATCACCAGCTGTGACCAGCAGCTCAGAAAGAATCCGTGGGAAACGGAGGGCCAAAGCC
  TTGAGATGGACAAGGCAGAAGTCGGTGGAGGAAGGGGAGCCACCAGGTCAGGGGGAAGGT
  CCCCGGTCCAGGCCAGCTGCTGAGTCCACCGGGCTGGAGGCCACATTCCCCAAGACCACA
  CCCTTGGCTCAAGCTGATCCTGCCGGGGTGGGCACTCCACCAACAGGGTGGGACTGCCTC
  CCCTCTGACTGTACAGCCTCAGCTGCAGGCTCCAGCACAGATGATGTGGAGCTGGCCACG
  GAGTTCCCAGCCACAGAGGCCTGGGAGTGTGAGCTAGAAGGCCTGCTGGAAGAGAGGCCT
  GCCCTGTGCCTGTCCCCGCAGGCCCCATTTCCCAAGCTGGGCTGGGATGACGAACTGCGG
  AAACCCGGCGCCCAGATCTACATGCGCTTCATGCAGGAGCACACCTGCTACGATGCCATG
  GCAACTAGCTCCAAGCTAGTCATCTTCGACACCATGCTGGAGATCAAGAAGGCCTTCTTT
  GCTCTGGTGGCCAACGGTGTGCGGGCAGCCCCTCTATGGGACAGCAAGAAGCAGAGCTTT
  GTGGGGATGCTGACCATCACTGACTTCATCCTGGTGCTGCATCGCTACTACAGGTCCCCC
  CTGGTCCAGATCTATGAGATTGAACAACATAAGATTGAGACCTGGAGGGAGATCTACCTG
  CAAGGCTGCTTCAAGCCTCTGGTCTCCATCTCTCCTAATGATAGCCTGTTTGAAGCTGTC
  TACACCCTCATCAAGAACCGGATCCATCGCCTGCCTGTTCTTGACCCGGTGTCAGGCAAC
  GTACTCCACATCCTCACACACAAACGCCTGCTCAAGTTCCTGCACATCTTTGGTTCCCTG
  CTGCCCCGGCCCTCCTTCCTCTACCGCACTATCCAAGATTTGGGCATCGGCACATTCCGA
  GACTTGGCTGTGGTGCTGGAGACAGCACCCATCCTGACTGCACTGGACATCTTTGTGGAC
  CGGCGTGTGTCTGCACTGCCTGTGGTCAACGAATGTGGTCAGGTCGTGGGCCTCTATTCC
  CGCTTTGATGTGATTCACCTGGCTGCCCAGCAAACCTACAACCACCTGGACATGAGTGTG
  GGAGAAGCCCTGAGGCAGAGGACACTATGTCTGGAGGGAGTCCTTTCCTGCCAGCCCCAC
  GAGAGCTTGGGGGAAGTGATCGACAGGATTGCTCGGGAGCAGGTACACAGGCTGGTGCTA
  GTGGACGAGACCCAGCATCTCTTGGGCGTGGTCTCCCTCTCCGACATCCTTCAGGCACTG
  GTGCTCAGCCCTGCTGGCATCGATGCCCTCGGGGCCTGAGAAGATCTGAGTCCTCAATCC
  CAAGCCACCTGCACACCTGGAAGCCAATGAAGGGAACTGGAGAACTCAGCCTTCATCTTC
  CCCCACCCCCATTTGCTGGTTCAGCTATGATTCAGGCTTCTTCAGCCCTCCCAAATTGCC
  CTTGCCCTACCTGTGCTCCCAGAAGCCCTCGGGCATGCCCAGTGCACCATGGGATGATGA
  AATTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017431 unedited
  TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCACAGTGTCGCA
  CACTTGCTCTCAGTTGGTCTGGGGCTGGCCACATGGAGCCCGGGCTGGAGCACGCACTGC
  GCAGGACCCCTTCCTGGAGCAGCCTTGGGGGTTCTGAGCATCAAGAGATGAGCTTCCTAG
  AGCAAGAAAACAGCAGCTCATGGCCATCACCAGCTGTGACCAGCAGCTCAGAAAGAATCC
  GTGGGAAACGGAGGGCCAAAGCCTTGAGATGGACAAGGCAGAAGTCGGTGGAGGAAGGGG
  AGCCACCAGGTCAGGGGGAAGGTCCCCGGTCCAGGCCAGCTGCTGAGTCCACCGGGCTGG
  AGGCCACATTCCCCAAGACCACACCCTTGGCTCAAGCTGATCCTGCCGGGGTGGGCACTC
  CACCAACAGGGTGGGACTGCCTCCCCTCTGACTGTACAGCCTCAGCTGCAGGCTCCAGCA
  CAGATGATGTGGAGCTGGCCACGGAGTTCCCAGCCACAGAGGCCTGGGAGTGTGAGCTAG
  AAGGCCTGCTGGAAGAGAGGCCTGCCCTGTGCCTGNTCCCGCANGCCCCATTTCCCAAGC
  TGGGCTGGGATGACGAACTGCGGAAACCCGGCGCCCAGATCTACATGCGCTTCATGCAGG
  AGCACACCTGCTACGATGCCATGGCAACTAGCTCCAAGCTAGTCATCTTCGACACCATGC
  TGGAGATCAAGAAGGCCTTTCTTGCTCTGGTGCCAACGGTGTGCGGGCAGCCCCTCTATG
  GGACAGCAGAAGCANAGCTTGTGGGGATGCTGACATCACTGACTCATCCTGGGCTGATCG
  CTACTACAGGTCCCCCTGTTCCGATCTTTGAGATTGACACATAAGATTGAACCTGGAGGG
  AAATCTACTGCAAGGCTGTTCAAGCCTCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_017431 unedited
  GCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCA
  AAAAAAACGGCACTTGGGATTTTTATCCATTTTCCTTCTAGTCTTTGAATTTAAAAAGCA
  CAAGTCTCTTGCCACAAGTTCAGGTCAAATGACTGCAGCATTTTGAGTGAAACCAATAAA
  ATCTAAGTTCATGGATTGGTCCTTTTGTTTTGTTTTCTCAATTGCTCATTCTCAGGGGTC
  CATATACAAGGCCATCCATCCCTGGAGACTAAAAACATGGCTCACTGCGAGAGCTAAGGA
  GTTTTCTGGACAAAAATGGAGGGAGGACTCCCCACCTCCAACAGGCAGTTCTATAAATAA
  CGGCTGCTCCATGGTGGCCCCTGAAATGCAGCATTTCAACAGCCTTGAAATGACAAACCC
  TGGAGAGTAGGAGGGCAGTGGCGACCTTGCTGGGAACAACAGCTGGGACTCCCAAGAGCT
  GAAAGCTGAACCACTAGGTTGCCCAAAAACCGAGGAATCCAAAAACCCTGACTAAACCCA
  CTGGGGCCATCCCACCCATTTCAGCGGGAAGGGGATGGGCGGCCCTGGAGCCCACATGGC
  CCTGGGGCTATCCTAACGCCTCTGGCTTAAGGACCCTTCATCTTTGACCAACCGTTCCCC
  TTTATTCCATCTCCCAGCCGCCTGCCCCTCCCCAGGCCTCTGGGCCCCCAGCCCTGCCAG
  GCCCTTACGACGGCCTTAAATCCTCCACCTCCTCTCCCTCTAAGCGTGCTCGGCCATACC
  ATCGCCTCTATCCACCATCCCTATTACGTCCCCACCTCTTCCGCCTCTTTCCTAGCCCTT
  ATTACTTCCCAACCTCTATCTATCACCATACCATTATCATACCTCTCTCCCCTTCCCACA
  ATTTTTCCCCTCACTTACCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_017431
• ORF Size: 1470 bp
• Insert Size: 2560
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_017431.2, NP_059127.2
• RefSeq Size: 2299
• RefSeq ORF: 1470
• Locus ID: 53632
• Domains: CBS
• Protein Families: Druggable Genome
• Protein Pathways: Adipocytokine signaling pathway, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway
• Gene Summary: The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. It is dominantly expressed in skeletal muscle. Studies of the pig counterpart suggest that this subunit may play a key role in the regulation of energy metabolism in skeletal muscle. [provided by RefSeq, Jul 2008]