RWDD3 (NM_015485) Human Untagged Clone

CAT#: SC114654

RWDD3 (untagged)-Human RWD domain containing 3 (RWDD3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RWDD3
• Synonyms: RSUME
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114654 sequence for NM_015485 edited (data generated by NextGen Sequencing)
  ATGGCGGAGCCTGTGCAGGAGGAGCTCTCGGTCCTGGCCGCGATTTTCTGCAGGCCCCAC
  GAGTGGGAGGTGCTGAGCCGCTCAGAGACAGATGGGACCGTGTTCAGAATTCACACAAAA
  GCTGAAGGATTTATGGATGCGGATATACCTCTGGAATTGGTGTTCCATTTGCCAGTCAAT
  TATCCTTCATGTCTACCTGGTATCTCGATTAACTCTGAACAGTTGACCAGGGCCCAGTGT
  GTGACTGTGAAAGAGAAGTTACTTGAGCAAGCAGAGAGCCTTTTGTCGGAGCCTATGGTT
  CATGAGCTGGTTCTCTGGATTCAGCAGAATCTCAGGCATATCCTCAGCCAACCAGAAACT
  GGCAGTGGCAGTGAAAAGTGTACTTTTTCAACAAGCACGACCATGGATGATGGATTGTGG
  ATAACTCTTTTGCATTTAGATCACATGAGAGCAAAGACTAAATATGTCAAAATTGTGGAG
  AAGTGGGCTTCAGATTTAAGGCTGACAGGAAGACTGATGTTCATGGGTAAAATAATACTG
  ATTTTACTACAGGGAGACAGAAACAACCTCAAGGNNNNNNNNNTTCTTCAGAAAACCTCC
  AAAGTAGATGTGGACTCAAGTGGAAAGAAATGCAAAGAGAAAATGATTAGTGTACTGTTT
  GAAACAAAAGTACAGACAGAACACAAAAGGTTTCTGGCATTTGAAGTCAAAGAGTATTCA
  GCGTTGGATGAATTACAAAAGGAATTTGAAACTGCAGGACTTAAGAAGCTTTTCTCCGAA
  TTTGTACTTGCTCTGGTAAAATGA
  
  Clone variation with respect to NM_015485.4
  258 t=>g;575 a=>n;576 g=>n;577 t=>n;578 a=>n;579 c=>n;580 t=>n;581 t=>n;582 g=>n;583 a=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015485 unedited
  NNNGGGTTCAAAATTTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGGGA
  AGCGCTGAGGCGGTGGGGCCCACAGCCATGGCGGAGCCTGTGCAGGAGGAGCTCTCGGTC
  CTGGCCGCGATTTTCTGCAGGCCCCACGAGTGGGAGGTGCTGAGCCGCTCAGAGACAGAT
  GGGACCGTGTTCAGAATTCACACAAAAGCTGAAGGATTTATGGATGCGGATATACCTCTG
  GAATTGGTGTTCCATTTGCCAGTCAATTATCCTTCATGTCTACCTGGTATCTCGATTAAC
  TCTGAACAGTTGACCAGGGCCCAGTGTGTGACTGTGAAAGAGAAGTTACTTGAGCAAGCA
  GAGAGCCTTTTGTCGGAGCCTATGGTTCATGAGCTGGTTCTCTGGATTCAGCAGAATCTC
  AGGCATATCCTCAGCCAACCAGAAACTGGCAGTGGCAGTGAAAAGTGTACTTTTTCAACA
  AGCACGACCATGGATGATGGATTGTGGATAACTCTTTTGCATTTAGATCACATGAGAGCA
  AAGACTAAATATGTCAAAATTGTGGAGAAGTGGGCTTCAGATTTAAGGCTGACAGGAAGA
  CTGATGTTCATGGGTAAAATAATACTGATTTTACTACAGGGAGACAGAAACAACCTCAAG
  GTGCCAAAAAGTTAAATGTTGAGTATGAATCTGGCTATTTTCTGCTTTAAATGGTGTGTC
  TTTAAGTGTGTTTTATAACAATGGGATAGATTAATTATTAAGATGTTTCTGCTTTCATTA
  TTACAATCTTAATGGATCTTCCTTTTCTTTTTAAAGAATGTCTGACTGCNTATTACAAGT
  ACAAACTTGCCAAGCTTGAAGATAAGATTGCATTTTAAAATCTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015485 unedited
  ACCGCGGGCCGCAATCTAGAGTCGAGGNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTAAAAAAATCTTTTATTTCCAAAAAAATTCCCCAAAAAATTCCTTTT
  TTTCAAACAAATTTTGTTTTTAAAAACAGGGGGCCTTTCCCCATTTTTTAAAATTTATTT
  TTAAAAAAAGGGCTAATTTACGGAACCTACCCTTTCCGGGAAAACATTAAAAAATACAAG
  TTTTTTTCAGGTTAAAAATTCCATAAAACAAAACGGGTTTTTGTCCCTGAAGTAAAAAGG
  AGCCCTATCTTTTGGCTTCAAAAAAACTTTAAAATGGCCTTTTTAAATAACCCCCCCCCC
  AAACCCAGGGCAAAACCAACAAAAAACCCGGTTTTTTTACAAAAAAATCCCGGGCTTCCA
  TTTCATTTACCCAAAGCAGGTCCAAATTCGGAAAAAAGCTTTTTAAGCCCTGCAGTTCCA
  AATCCCTTTGGAAATCCACCCAACGCTGAAAACTTTTTGACTTCAAAGGCCAAAACCCTG
  CTTTATACAAAAAAACCTCAGGAATATCCCTAGATTTGCCAAAGTATAATTATGGATTCA
  GTGGCTTTTCCATCGGCAAAAGAACTAAATTATCCCTTTGGGGTTCGGCCTGACCTTTGG
  TTACAAACAGAACACTAATCATTTTCTTTTTGCATTTCTTTCCCCTGGAGCCCACATTTA
  CTTGTGGAGGTTTTCCTGAAGAATCAAGGCCCTCCTAAATAAATAACCCGGCACCTTTAA
  AATCCTTTCTTTACTTTGAGCCCCAAAGGCCCCAGTTTAAAAGAAGCTTTAACACGTGGA
  GCGTTATTTTCATGCTTAAAACCCCCCTTTTTAAACCAAGCAGGAAGAATTTTATATCCT
  TGTTTACAATCCTGGGCAGAAAAGGGTAGGGGCGGTCCCGGAATAAAGACAATATACCCA
  CCTCCCAGGNTTAAACGGGATGACCGCTACAAGTTCTACCTGGGCGGGCACTAAAACTGT
  GGGACAGCGCCTTCCAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015485
• ORF Size: 588 bp
• Insert Size: 3270
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_015485.3, NP_056300.1
• RefSeq Size: 1093
• RefSeq ORF: 588
• Locus ID: 25950
• Domains: RWD
• Gene Summary: Enhancer of SUMO conjugation. Via its interaction with UBE2I/UBC9, increases SUMO conjugation to proteins by promoting the binding of E1 and E2 enzymes, thioester linkage between SUMO and UBE2I/UBC9 and transfer of SUMO to specific target proteins which include HIF1A, PIAS, NFKBIA, NR3C1 and TOP1. Isoform 1 and isoform 2 positively regulate the NF-kappa-B signaling pathway by enhancing the sumoylation of NF-kappa-B inhibitor alpha (NFKBIA), promoting its stabilization which consequently leads to an increased inhibition of NF-kappa-B transcriptional activity. Isoform 1 and isoform 2 negatively regulate the hypoxia-inducible factor-1 alpha (HIF1A) signaling pathway by increasing the sumoylation of HIF1A, promoting its stabilization, transcriptional activity and the expression of its target gene VEGFA during hypoxia. Isoform 2 promotes the sumoylation and transcriptional activity of the glucocorticoid receptor NR3C1 and enhances the interaction of SUMO1 and NR3C1 with UBE2I/UBC9. Has no effect on ubiquitination. [UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (a).