Gemin 3 (DDX20) (NM_007204) Human Untagged Clone

CAT#: SC115683

DDX20 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (DDX20)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DDX20
• Synonyms: DP103; GEMIN3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115683 sequence for NM_007204 edited (data generated by NextGen Sequencing)
  ATGGCGGCGGCATTTGAAGCCTCGGGAGCCTTAGCAGCAGTGGCGACTGCTATGCCGGCT
  GAGCATGTGGCCGTGCAGGTCCCGGCCCCAGAGCCAACACCCGGGCCTGTGAGGATCCTG
  CGGACCGCTCAGGATCTCAGCAGCCCGCGGACCCGCACGGGGGATGTGCTGTTGGCGGAG
  CCGGCCGACTTCGAGTCACTGCTGCTTTCGCGGCCGGTGCTGGAGGGGCTGCGGGCGGCC
  GGCTTCGAGAGGCCCTCGCCGGTGCAGCTCAAGGCCATCCCGTTGGGGCGCTGCGGGCTC
  GATTTAATTGTTCAAGCTAAATCTGGCACCGGGAAAACCTGTGTGTTCTCCACCATAGCT
  TTGGACTCTCTTGTTCTTGAAAACTTAAGTACCCAGATTTTGATCTTGGCTCCTACAAGA
  GAAATTGCTGTACAGATACATTCTGTTATTACAGCCATTGGAATAAAAATGGAAGGCTTA
  GAGTGTCATGTCTTTATTGGAGGGACCCCATTATCACAAGACAAAACCAGACTTAAAAAG
  TGTCATATTGCTGTTGGATCTCCTGGCAGAATTAAGCAACTCATAGAACTTGACTACTTG
  AACCCAGGCAGTATACGCCTCTTTATTCTTGATGAAGCAGATAAGCTTTTAGAAGAAGGC
  AGCTTCCAGGAGCAAATAAATTGGATTTATTCTTCCTTGCCTGCCAGTAAACAGATGCTG
  GCAGTATCAGCTACTTATCCCGAATTTTTGGCTAATGCTTTGACAAAGTACATGAGAGAT
  CCCACTTTTGTAAGACTGAATTCCAGTGATCCAAGTCTCATAGGTTTGAAGCAGTATTAC
  AAAGTTGTCAATTCATACCCTTTGGCACATAAGGTTTTTGAGGAAAAGACTCAGCATTTA
  CAGGAACTGTTCAGCAGAATTCCATTTAATCAAGCTTTAGTCTTTTCTAATTTGCACAGC
  AGAGCACAACATTTGGCTGATATCCTTTCTTCTAAAGGCTTTCCTGCTGAGTGCATTTCA
  GGCAATATGAATCAGAATCAGCGTCTTGATGCTATGGCTAAACTGAAGCACTTTCATTGC
  AGAGTCCTCATTTCCACAGATTTGACTTCTCGTGGGATTGATGCTGAGAAGGTGAATCTG
  GTTGTAAATCTGGATGTACCATTGGATTGGGAGACATACATGCATCGGATTGGGAGAGCT
  GGCCGTTTTGGTACATTGGGGCTGACAGTGACCTACTGTTGCCGGGGAGAGGAAGAAAAT
  ATGATGATGAGAATTGCCCAGAAATGTAATATCAACCTTCTCCCTTTACCAGATCCCATT
  CCTTCTGGTCTGATGGAAGAATGTGTGGATTGGGATGTGGAAGTTAAAGCTGCTGTGCAT
  ACATATGGTATAGCAAGTGTACCTAACCAACCCTTAAAAAAGCAAATTCAGAAAATAGAG
  AGAACCCTTCAAATTCAGAAAGCTCATGGTGACCACATGGCTTCCTCTAGAAATAATTCT
  GTATCTGGACTATCAGTCAAATCAAAAAATAATACCAAACAAAAGCTTCCTGTGAAAAGC
  CACTCAGAATGTGGAATCATAGAAAAAGCAACGTCACCAAAAGAACTGGGCTGTGACAGG
  CAATCCGAAGAGCAAATGAAGAATTCTGTTCAGACTCCCGTTGAAAACTCCACCAACAGT
  CAGCACCAGGTCAAAGAAGCTTTACCTGTGTCACTCCCCCAGATTCCTTGTCTGTCTTCC
  TTTAAAATCCATCAGCCATACACGTTGACTTTTGCTGAATTGGTAGAGGATTATGAACAT
  TATATTAAAGAGGGGTTAGAGAAACCTGTGGAAATCATCAGGCACTACACAGGCCCTGGG
  GATCAGACTGTGAATCCTCAAAATGGTTTTGTGAGAAATAAAGTTATTGAACAGAGAGTC
  CCTGTGTTGGCAAGTAGTAGCCAATCTGGAGACTCTGAGAGTGACAGTGATTCTTACAGC
  TCAAGAACCTCTTCCCAGAGCAAAGGAAATAAGTCATACTTGGAAGGCTCTTCTGATAAT
  CAGCTGAAAGACTCTGAATCTACGCCTGTGGATGATCGTATTTCTTTGGAACAACCACCA
  AATGGAAGTGACACCCCCAATCCAGAGAAATATCAAGAATCACCTGGAATCCAGATGAAG
  ACAAGACTTAAAGAGGGGGCTAGCCAGAGAGCTAAGCAGAGCCGGAGAAACCTACCCAGG
  CGGTCTTCCTTCAGATTGCAGACTGAAGCCCAGGAAGATGATTGGTATGACTGTCATAGG
  GAAATACGTCTGAGTTTTTCTGATACCTATCAGGATTATGAGGAGTACTGGAGAGCTTAC
  TACAGGGCATGGCAAGAATATTATGCTGCCGCTTCTCATTCATATTATTGGAATGCTCAG
  AGACATCCAAGTTGGATGGCAGCTTATCACATGAATACCATTTATCTACAAGAAATGATG
  CATAGTAACCAGTGA
  
  Clone variation with respect to NM_007204.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007204 unedited
  ATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGAGATCTGACG
  GCGCGGCTACCATGGCGGCGGCATTTGAAGCCTCGGGAGCCTTAGCAGCAGTGGCGACTG
  CTATGCCGGCTGAGCATGTGGCCGTGCAGGTCCCGGCCCCAGAGCCAACACCCGGGCCTG
  TGAGGATCCTGCGGACCGCTCAGGATCTCAGCAGCCCGCGGACCCGCACGGGGGATGTGC
  TGTTGGCGGAGCCGGCCGACTTCGAGTCACTGCTGCTTTCGCGGCCGGTGCTGGAGGGGC
  TGCGGGCGGCCGGCTTCGAGAGGCCCTCGCCGGTGCAGCTCAAGGCCATCCCGTTGGGGC
  GCTGCGGGCTCGATTTAATTGTTCAAGCTAAATCTGGCACCGGGAAAACCTGTGTGTTCT
  CCACCATAGCTTTGGACTCTCTTGTTCTTGAAAACTTAAGTACCCAGATTTTGATCTTGG
  CTCCTACAAGAGAAATTGCTGTACAGATACATTCTGTTATTACAGCCATTGGAATAAAAA
  TGGAAGGCTTAGAGTGTCATGTCTTTATTGGAGGGACCCCATTATCACAAGACAAAACCA
  GACTTAAAAAGTGTCATATTGCTGTTGGATCTCCTGGCAGAATTAAGCAACTCATAGAAC
  TTGACTACTTGAACCCAGGCAGTATACGCCTCTTTATTCTTGATGAAGCAGATAAGCTNT
  TAGAAGAAGGCAGCTTCCAGGAGCAAATAAATTGGATTTATTCTTNCCTGGCCTGCCAGT
  AAACAGATGCTGGCAGTATCAGCTACTTATCCCCGAATTTTGGNNCTATGGCTTGACAAA
  GTACATGAGAGACCCACTTTTGTAGACTGAATNCAGTGATCCAGTCTCATAGGTTTGAGC
  AGTATACAAAGTGTCAATTCTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007204 unedited
  CGCGGCCGCAATCTAGNATCGAGGTTTTTTTTTTTTTTTTTTGCTAATCAAATCTATTGC
  TTTTATTGTTTTTAAGTAAGTGGTGGTATATGTCATCTTTTTAAATGTTTCTTTTGGGAA
  GATCTTATAGAAAATGGCACCCTGAAACTTTCAGCTCTATGTCCCAAAAATTATCTTTCA
  ACAATCTGATGAAGTTTCTTAAAGAAACTCTTAAAAATGAAGATAATATACCTGGCCTAA
  ATCAAAACAATCTGAAAAATGGATGTGTCCCAGTGGAAAGACTCAAGTCTTTTCAAGTTT
  ATCAGAAATGCCACTATACACCACTGTACTATAAGTCGAGGAGGATGGATATCCAAAGGT
  ATCATTTGTTGACAGTTCCTGATGGTCTCAGGTATATCCTATAATCACTGGTTACTATGC
  ATCATTTCTTGTAGATAAATGGTATTCATGTGATAAGCTGCCATCCAACTTGGATGTCTC
  TGAGCATTCCAATAATATGAATGAGAAGCGGCAGCATAATATTCTTGCCATGCCCTGTAG
  TAAGCTCTCCAGTACTCCCCATAATCCTGATAGGTATCAAAAAAACCCACACCTATTTCC
  CTATGACAGTCCTACCAATCATCTTCCTGGGCTTAAGTCTGCAATCCGAAGGAAGACCGC
  CTGGGTAGGTTTCCTCCGCTCCTGCTTACCTCTCTGGCTCGCCCCCTCTTTAGTCCTGCC
  TCCATCTGGACTCCCAGGGATCCTTGGTATTCCTCTGGATCGCGGGCGGCCCCTCCCTCC
  GCCGGTGCGTCCCAAAAACACCACCATCCCCCGGCCCAAGAACCAAGACCTCCACCCGCA
  CTTACCACAATCCCCTCACGTCTCAACTCTCTCCCCTCCTCGCGGCCAGAGGGTCCCACC
  CCCCACCATCCACGGTACCTCCAGACGCCCCACAGCCTTTCCCCTGCGCACCCCCACCCT
  C
  
• Restriction Sites: NotI-NotI
• ACCN: NM_007204
• ORF Size: 2475 bp
• Insert Size: 2800
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_007204.3, NP_009135.3
• RefSeq Size: 3449
• RefSeq ORF: 2475
• Locus ID: 11218
• Domains: DEAD, helicase_C
• Protein Families: Druggable Genome
• Gene Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which has an ATPase activity and is a component of the survival of motor neurons (SMN) complex. This protein interacts directly with SMN, the spinal muscular atrophy gene product, and may play a catalytic role in the function of the SMN complex on RNPs. [provided by RefSeq, Jul 2008]