MCM5 (NM_006739) Human Untagged Clone

CAT#: SC115899

MCM5 (untagged)-Human minichromosome maintenance complex component 5 (MCM5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MCM5
• Synonyms: CDC46; MGORS8; P1-CDC46
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006739, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCGGGATTCGACGATCCTGGCATTTTCTACAGCGACAGCTTCGGGGGCGACGCCCAGGCCGACGAGG
  GGCAGGCCCGCAAATCGCAGCTGCAGAGGCGCTTCAAGGAGTTCCTGCGGCAGTACCGAGTGGGCACCGA
  CCGCACGGGCTTCACCTTCAAATACAGGGATGAACTCAAGCGGCATTACAACCTGGGGGAGTACTGGATT
  GAGGTGGAGATGGAGGATCTGGCCAGCTTTGATGAGGACCTGGCCGACTACTTGTACAAGCAGCCAGCCG
  AGCACCTGCAGCTGCTGGAGGAAGCTGCCAAGGAGGTAGCTGATGAGGTGACCCGGCCCCGGCCTTCTGG
  GGAGGAGGTGCTCCAGGACATCCAGGTCATGCTCAAGTCGGACGCCAGCCCTTCCAGCATTCGTAGCCTG
  AAGTCGGACATGATGTCACACCTGGTGAAGATCCCTGGCATCATCATCGCGGCCTCTGCGGTCCGTGCCA
  AGGCCACCCGCATCTCTATCCAGTGCCGCAGCTGCCGCAACACCCTCACCAACATTGCCATGCGCCCTGG
  CCTCGAGGGCTATGCCCTGCCCAGGAAGTGCAACACAGATCAGGCTGGGCGCCCCAAATGCCCATTGGAC
  CCGTACTTCATCATGCCCGACAAATGCAAATGCGTGGACTTCCAGACCCTGAAGCTGCAGGAGCTGCCTG
  ATGCAGTCCCCCACGGGGAGATGCCCAGACACATGCAGCTCTACTGCGACAGGTACCTGTGTGACAAGGT
  CGTCCCTGGGAACAGGGTTACCATCATGGGCATCTACTCCATCAAGAAGTTTGGCCTGACTACCAGCAGG
  GGCCGTGACAGGGTGGGCGTGGGCATCCGAAGCTCCTACATCCGTGTCCTGGGCATCCAGGTGGACACAG
  ATGGCTCTGGCCGCAGCTTTGCTGGGGCCGTGAGCCCCCAGGAGGAGGAGGAGTTCCGTCGCCTGGCTGC
  CCTCCCAAATGTCTATGAGGTCATCTCCAAGAGCATCGCCCCCTCCATCTTTGGGGGCACAGACATGAAG
  AAGGCCATTGCCTGCCTGCTCTTTGGGGGCTCCCGAAAGAGGCTCCCTGATGGACTTACTCGCCGAGGAG
  ACATCAACCTGCTGATGCTAGGGGACCCTGGGACAGCCAAGTCCCAGCTTCTGAAGTTTGTGGAGAAGTG
  TTCTCCCATTGGGGTATACACGTCTGGGAAAGGCAGCAGCGCAGCTGGACTGACAGCCTCGGTGATGAGG
  GACCCTTCGTCCCGGAATTTCATCATGGAGGGCGGAGCCATGGTCCTGGCCGATGGTGGGGTCGTCTGTA
  TTGACGAGTTTGACAAGATGCGAGAAGATGACCGTGTGGCAATCCACGAAGCCATGGAGCAGCAGACCAT
  CTCTATCGCCAAGGCTGGGATCACCACCACCCTGAACTCCCGCTGCTCCGTCCTGGCTGCTGCCAACTCA
  GTGTTCGGCCGCTGGGATGAGACGAAGGGGGAGGACAACATTGACTTCATGCCCACCATCTTGTCGCGCT
  TCGACATGATCTTCATCGTCAAGGATGAGCACAATGAGGAGAGGGATGTGATGCTGGCCAAGCATGTCAT
  CACTCTGCACGTGAGCGCACTGACACAGACACAGGCTGTGGAGGGCGAGATTGACCTGGCCAAGCTGAAG
  AAGTTTATTGCCTACTGCCGAGTGAAGTGTGGCCCCCGGCTGTCAGCAGAGGCTGCAGAGAAACTGAAGA
  ACCGCTACATCATCATGCGGAGCGGGGCCCGTCAGCACGAGAGGGACAGTGACCGCCGCTCCAGCATCCC
  CATCACTGTGCGGCAGCTGGAGGCCATTGTGCGCATCGCGGAAGCCCTCAGCAAGATGAAGCTGCAGCCC
  TTCGCCACAGAGGCAGATGTGGAGGAGGCCCTGCGGCTCTTCCAAGTGTCCACGTTGGATGCTGCCTTGT
  CCGGTACCCTGTCAGGGGTGGAGGGCTTCACCAGCCAGGAGGACCAGGAGATGCTGAGCCGCATCGAGAA
  GCAGCTCAAGCGCCGCTTTGCCATTGGCTCCCAGGTGTCTGAGCACAGCATCATCAAGGACTTCACCAAG
  CAGAAATACCCGGAGCACGCCATCCACAAGGTGCTGCAGCTCATGCTGCGGCGCGGCGAGATCCAGCATC
  GCATGCAGCGCAAGGTTCTCTACCGCCTCAAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006739 unedited
  GTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGNATTCGGCACGAGGCTCCCC
  TGGTTTGTGAAGTGCGNGAAAACCANAGGCGCAGTCATGTCGGGATTCGACGATCCTGGC
  ATTTTCTACAGCGACAGCTTCGGGGGCGACGCCCAGGCCGACGAGGGGCAGGCCCGCAAA
  TCGCAGCTGCAGAGGCGCTTCAAGGAGTTCCTGCGGCAGTACCGAGTGGGCACCGACCGC
  ACGGGCTTCACCTTCAAATACAGGGATGAACTCAAGCGGCATTACAACCTGGGGGAGTAC
  TGGATTGAGGTGGAGATGGAGGATCTGGCCAGCTTTGATGAGGACCTGGCCGACTACTTG
  TACAAGCAGCCAGCCGAGCACCTGCAGCTGCTGGAGGAAGCTGCCAAGGAGGTAGCTGAT
  GAGGTGACCCGGCCCCGGCCTTCTGGGGAGGAGGTGCTCCAGGACATCCAGGTCATGCTC
  AAGTCGGACGCCAGCCCTTCCAGCATTCGTAGCCTGAAGTCGGACATGATGTCACACCTG
  GTGAAGATCCCTGGCATCATCATCGCGGCCTCTGCGGTCCGTGCCAAGGCCACCCGCATC
  TCTATCCAGTGCCGCAGCTGCCGCAACACCCTCACCAACATTGTCATGCGCCCTGTGCTC
  TAGGGCTATGCCCTGCCCAAGAAGTGCAACACAGATCANGCTGGGCGCCCCAAATGCCCA
  TTGGACCCGTACTTCATCATGCCCGACAAATGCAAATGCGTGGACTTCCAGACCCTGAAG
  CTGCAGGAGCTGCCTGATGCAGTCCCCCACGGGGAGATGCCCAGACACATGCAGCTCTAC
  TGCGACAGGTACCTGTGTGANCAGGTCGTNCCTGGGAACAGGNTTACCATCATGGGCATC
  TACTCATTCTAGAAAGTTGGCCTGACTACCANNCAGGGCCGTGACAGGNTGGGGCGTGGG
  CATCCCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006739 unedited
  TATGCCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCCGAACACCCCGGGGGTTTTTT
  TTTAACGGGGGCCAAAAGCCCCCTTCCCAAAACCCCCCTAAAGGGGGGCCCCCAAAGGCA
  GCAGGACACTACAGCTCCTTTCTTTGGGGCAAAAAGGAGGGGGCCTGGGAAATTCGAGGG
  CTGCAGGGGGGAGGCAAAGGTCCCAACAACATTTTCAATGGCAGGCACCGGCAGGAGGGG
  CAAGGCGTGGGCAAATCCATGAATCCAGGGGGGGGGGCCCACTCACTTGAGGGGGGAAAA
  AACCTTGCCCTGCATGCAAAGCTGGATCTCCCCCCCCCCCAAAATGAACTGCAACACCTT
  GGGGAGGGGGGGCCCCGGGTTTTTCTGCTTGGGGAAACCCTTGATGAAGCTGGGCTCAAA
  CCCCTGGGAACCAATGGCAAAACGGGGCTTGAACTGTTTTTTAAAGCGGGTAAAAATTTC
  CTGGGCCTCCTGGGGGGGGAAACCCTCCACCCCTGACAGGGGACCGGACAAGGGAACATC
  CAACGGGGACACTTGGAAAAACCGCAGGGCCTCCTCCAATTTGCCTTTGGGGCGAAAGGC
  TGCAACTTCATCTTGCTGAGGGGCTCCGGGAAGCGCCAATGGCCTTCAACTGCCCAACAT
  GATGGGGAATCTTGAACGGGGGTCACTTGTCCTCTTGTGCTGAGGGCCCCCTTTCGCATG
  GAGAAGAAACGGGTCTTTATTTTTTTGGACCTTTGGTAAAGCGGGGGCCCCCTTCATTGG
  GAGAAGCAAAAAACTTTTAACTGGCCAGGAAATCCCCTTCCAGCCTGGGTTGGTACAAGC
  CCCC
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_006739
• Insert Size: 2600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006739.2, NP_006730.2
• RefSeq Size: 2548 bp
• RefSeq ORF: 2205 bp
• Locus ID: 4174
• Cytogenetics: 22q12.3
• Domains: MCM
• Protein Families: Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Cell cycle, DNA replication
• Gene Summary: 'The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation. [provided by RefSeq, Jul 2008]'