TRIM27 (NM_006510) Human Untagged Clone

CAT#: SC116046

TRIM27 (untagged)-Human tripartite motif containing 27 (TRIM27)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TRIM27
• Synonyms: RFP; RNF76
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006510 edited
  GAATTCGGCACGAGGCCCCCGCAGGCCGGCGCCATGGCCTCCGGGAGTGTGGCCGAGTGC
  CTGCAGCAGGAGACCACCTGCCCCGTGTGCCTGCAGTACTTCGCAGAGCCCATGATGCTC
  GACTGCGGCCATAACATCTGTTGCGCGTGCCTCGCCCGCTGCTGGGGCACGGCAGAGACT
  AACGTGTCGTGCCCGCAGTGCCGGGAGACCTTCCCGCAGAGGCACATGCGGCCCAACCGG
  CACCTGGCCAACGTGACCCAACTGGTAAAGCAGCTGCGCACCGAGCGGCCGTCGGGGCCC
  GGCGGCGAGATGGGCGTGTGCGAGAAGCACCGCGAGCCCCTGAAGCTGTACTGCGAGGAG
  GACCAGATGCCCATCTGCGTGGTGTGCGACCGCTCCCGCGAGCACCGCGGCCACAGCGTG
  CTGCCGCTCGAGGAGGCGGTGGAGGGCTTCAAGGAGCAAATCCAGAACCAGCTCGACCAT
  TTAAAAAGAGTGAAAGATTTAAAGAAGAGACGTCGGGCCCAGGGGGAACAGGCACGAGCT
  GAACTCTTGAGCCTAACCCAGATGGAGAGGGAGAAGATTGTTTGGGAGTTTGAGCAGCTG
  TATCACTCCTTAAAGGAGCATGAGTATCGCCTCCTGGCCCGCCTTGAGGAGCTAGACTTG
  GCCATCTACAATAGCATCAATGGTGCCATCACCCAGTTCTCTTGCAACATCTCCCACCTC
  AGCAGCCTGATCGCTCAGCTAGAAGAGAAGCAGCAGCAGCCCACCAGGGAGCTCCTGCAG
  GACATTGGGGACACATTGAGCAGGGCTGAAAGAATCAGGATTCCTGAACCTTGGATCACA
  CCTCCAGATTTGCAAGAGAAAATCCACATTTTTGCCCAAAAATGTCTATTCTTGACGGAG
  AGTCTAAAGCAGTTCACAGAAAAAATGCAGTCAGATATGGAGAAAATCCAAGAATTAAGA
  GAGGCTCAGTTATACTCAGTGGACGTGACTCTGGACCCAGACACGGCCTACCCCAGCCTG
  ATCCTCTCTGATAATCTGCGGCAAGTGCGGTACAGTTACCTCCAACAGGACCTGCCTGAC
  AACCCCGAGAGGTTCAATCTGTTTCCCTGTGTCTTGGGCTCTCCATGCTTCATCGCCGGG
  AGACATTATTGGGAGGTAGAGGTGGGAGATAAAGCCAAGTGGACCATAGGTGTCTGTGAA
  GACTCAGTGTGCAGAAAAGGTGGAGTAACCTCAGCCCCCCAGAATGGATTCTGGGCAGTG
  TCTTTGTGGTATGGGAAAGAATATTGGGCTCTTACCTCCCCAATGACTGCCCTACCCCTG
  CGGACCCCGCTCCAGCGGGTGGGGATTTTCTTGGACTATGATGCTGGTGAGGTCTCCTTC
  TACAACGTGACAGAGAGGTGTCACACCTTCACTTTCTCTCATGCTACCTTTTGTGGGCCT
  GTCCGGCCCTACTTCAGTCTGAGTTACTCGGGAGGGAAAAGTGCAGCTCCTCTGATCATC
  TGCCCCATGAGTGGGATAGATGGGTTTTCTGGCCATGTTGGGAATCATGGTCATTCCATG
  GAGACCTCCCCTTGAGGAGGTGAATTCAGGCCAAAAGGGCTGTTGGCTGTAATCCTACGC
  CAGGCACAAGGCATCTTGTTGCCTTGCCACGTCCTGTCACAGCTGGGTATCCTTACCATG
  TTCCACGCCCTTGCAGTGGGAGACAGGATGTCCATGTTCTCTACCATCCTTTTCCTTCCC
  ATGCAGATTGTGAAATGTAATGAGATGTATCAAGATATCCTAGAAATAAAAACCAGATGT
  CCACCTCCAGTGTTTCATACTTTCTGGTTTTACACATCGCTGGAGGGATAAAGAGTATGG
  ATAATCTTTGGATTTGGAGAGCCGTTCAAGATACTTCCAGCTTCTTGGCTCAGCCTGGCT
  TCCTCTGGTTCAGCCCCACATAATGATTATGGCTATTTGCTGTCATTTCTGGGCTAGGGC
  TCCTTTCTAACAACCTAGACTGGAATAAGGCCCTGTCAGCATGGCTCCCTTTATCCCAGT
  TTTCCGTCTGGGAACAGTACCTCTGCCCCTGATTCCCAATGTGCCATAGTTTTATTAACT
  CCATTAAAGAAGCCTGTATGTGTTTTGGTTAGTTACAGTTATTTTACAATAATGGTGGGT
  AATGGCCCCACCTCTGTTATGAGATAATGTTCTAATCAATGTCTCTGCCTTTGTATCTTT
  TCTGAGGGCTTTGTCTGTTCTCTTCATTCTAATGAAAGGTGTATTCTAGTGCTGGGTGCA
  TATCATCCAGGATAATATTCTGCCCAACTCCATCCTCTGTTACTAGATCCCTTACCAGTC
  ACATTTGTGGACTGGTGGCCAGTCGTATACCATCCCTGGAAGGATTCTGGGACAATATTC
  CAGGGATTCATTGACTTCTTGGCTCCTTTTCTCCATTTCCTTTGGGGGAAGGGGGAATTG
  ACCATGCTTAAGTGCATCCTATCAAGGGGCAGCTCCGTCCCCATGGCCATTGGATCATGA
  GACACTCTGAAGTCAGAAGGCTGGGGCAGATCACTTCAAGCAAGCCCCCATGATGGTTCT
  CAGTCCTGCTTCTCTGTGGGTACGTGCCCCTCTGTTTAAAAATAAACTGAATATGGATGT
  TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006510 unedited
  GGGTACTATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGCCCCCGCA
  GGCCGGCGCCATGGCCTCCGGGAGTGTGGCCGAGTGCCTGCAGCAGGAGACCACCTGCCC
  CGTGTGCCTGCAGTACTTCGCAGAGCCCATGATGCTCGACTGCGGCCATAACATCTGTTG
  CGCGTGCCTCGCCCGCTGCTGGGGCACGGCAGAGACTAACGTGTCGTGCCCGCAGTGCCG
  GGAGACCTTCCCGCAGAGGCACATGCGGCCCAACCGGCACCTGGCCAACGTGACCCAACT
  GGTAAAGCAGCTGCGCACCGAGCGGCCGTCGGGGCCCGGCGGCGAGATGGGCGTGTGCGA
  GAAGCACCGCGAGCCCCTGAAGCTGTACTGCGAGGAGGACCAGATGCCCATCTGCGTGGT
  GTGCGACCGCTCCCGCGAGCACCGCGGCCACAGCGTGCTGCCGCTCGAGGAGGCGGTGGA
  GGGCTTCAAGGAGCAAATCCAGAACCAGCTCGACCATTTAAAAAGAGTGAAAGATTTAAA
  GAAGAGACGTCGGGCCCAGGGGGAACAGGCACGAGCTGAACTCTTGAGCCTAACCCAGAT
  GGAGAGGGAGAAGATTGTTTGGGAGTTTGAGCAGCTGTATCACTCCTTAAAGGAGCATGA
  GTATCGCCTCCTGGCCCGCCTTGAGGAGCTAGACTTGGCCATCTACAATAGCATCAATGG
  TGCCATCACCCCAGTTCTCTTGCAACATCTCCCACCTCAGCAGCCTGATCGCTCACCTAG
  AAGAGAAGCAGCACCAGCCCACCCAGGAGCTCCTGCCAGACCATTGGGGACACATTGAGC
  AGGGCTGAAAGGATACGGATCCTGAACCTTGAATACACCTCCAGATTGCAAGAGAAATAC
  CCATTTTGCCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006510 unedited
  TTTTAAAAANNCCTCTGTGNACCGCGCCGCATTCTANGATCGATNTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTAAACATCCATATTCAGTTTATTTTTAAACAGAGGGGCACGTACCCAC
  AGAGAAGCAGGACTGAAAACCATCATGGGGGCTTGCTTGAAGTGATCTGCCCCAGCCTTC
  TGACTTCAAAGTGTCTCATGATCCAATGGCCATGGGGACGGAGCTGCCCCTTGATAGGAT
  GCACTTAAGCATGGTCAATTCCCCCTTCCCCCAAAGGAAATGGAGAAAAGGAGCCAAGAA
  GTCAATGAATCCCCTGGAATATTGTCCCAGAATCCTTCCAGGGATGGTATACGACTGGCC
  ACCAGTCCACAAATGTGACTGGGTAGGGATCTAGTAACAGAGGATGGAGTTGGGCAGAAT
  ATTATCCTGGATGATATGCACCCAGCACTAGAATACACCTTTCATTAGAATGAAGAGAAC
  GACAAAGCCCTCAAAAAAGATACAAAAGCAAAGACATTGATTAGAACATTATCTCATAAC
  AGAAGTTGGGGCCATTACCCACCATTATTGGAAAATACCTGGAAACTAACCAAAACCCTT
  ACAAGGCTTCTTTTNTGGGAGTGTAATAAAACTATTGGCCCATTGGNGAAATCAGGGGCC
  AAAAGTACTTGTTCCCCNACCGGAAAACCTGGGATAAAGGGGAACCCTTCCTGAAAGGGC
  CCTTATTCTCAGTCTTAGGGTTGTTTAAAGAGAGACCCTACCCCCANAAATGCACCCCAA
  ATATCCTTTATTCCTTATGTGGGGCGCTGAACCCAAGAGAAACCACGGTTTACCCCAAAA
  ACCTGGAATTTTCTTAAACGGCCTCTCACAACCCAAAGATTTTCCATCCTTTTTATTCCC
  TTCCCCGATGGTGTAAACCCCAAAAATTGAAAACCTGGGGGGGGGCCACTGGGGTTTTTA
  TTTGAGAGATTTGGGACCCTTTTTTAACTTTAACAATTGGGGGGGAAGAGAAAGAGGGGG
  NAAAAAATTGGCCTTTCTTCCCCCTTGAGAGGGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006510
• ORF Size: 1542 bp
• Insert Size: 2770
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_006510.3, NP_006501.1
• RefSeq Size: 2984
• RefSeq ORF: 1542
• Locus ID: 5987
• Domains: zf-B_box, RING, SPRY, PRY
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (alpha) lacks 280 nt in the coding region, resulting in a frameshift and use of an upstream stop codon. It encodes isoform alpha which has a distinct C-terminus as compared to isoform beta.