RAD21 (NM_006265) Human Untagged Clone

CAT#: SC116225

RAD21 (untagged)-Human RAD21 homolog (S. pombe) (RAD21)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAD21
• Synonyms: CDLS4; hHR21; HR21; HRAD21; MCD1; MGS; NXP1; SCC1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116225 sequence for NM_006265 edited (data generated by NextGen Sequencing)
  ATGTTCTACGCACATTTTGTTCTCAGTAAAAGAGGGCCTCTGGCCAAAATTTGGCTAGCG
  GCCCATTGGGATAAGAAGCTAACCAAAGCCCATGTGTTCGAGTGTAATTTAGAGAGCAGC
  GTGGAGAGTATCATCTCACCAAAGGTGAAAATGGCATTACGGACATCAGGACATCTCTTA
  CTGGGAGTAGTTCGAATCTATCACAGGAAAGCCAAATACCTTCTTGCAGACTGTAATGAA
  GCATTCATTAAGATAAAGATGGCTTTTCGGCCAGGTGTGGTTGACCTGCCTGAGGAAAAT
  CGGGAAGCAGCTTATAATGCCATTACTTTACCTGAAGAATTTCATGACTTTGATCAGCCA
  CTGCCTGACTTAGATGACATCGATGTGGCCCAGCAGTTCAGCTTGAATCAGAGTAGAGTG
  GAAGAGATAACCATGAGAGAAGAAGTTGGGAACATCAGTATTTTACAAGAAAATGATTTT
  GGTGATTTTGGAATGGATGATCGTGAGATAATGAGAGAAGGCAGTGCTTTTGAGGATGAC
  GACATGTTAGTAAGCACTACTACTTCTAACCTCCTATTAGAGTCTGAACAGAGCACCAGC
  AATCTGAATGAGAAAATTAACCATTTAGAATATGAAGATCAATATAAGGATGATAATTTT
  GGAGAAGGAAATGATGGTGGAATATTAGATGACAAACTTATTAGTAATAATGATGGCGGT
  ATCTTTGATGATCCCCCTGCCCTCTCTGAGGCAGGGGTGATGTTGCCAGAGCAGCCTGCA
  CATGACGATATGGATGAGGATGATAATGTATCAATGGGTGGGCCTGATAGTCCTGATTCA
  GTGGATCCCGTTGAACCAATGCCAACCATGACTGATCAAACAACACTTGTTCCAAATGAG
  GAAGAAGCATTTGCATTGGAGCCTATTGATATAACTGTTAAAGAAACAAAAGCCAAGAGG
  AAGAGGAAGCTAATTGTTGACAGTGTCAAAGAGTTGGATAGCAAGACAATTAGAGCCCAA
  CTTAGTGATTATTCAGATATTGTTACTACTTTGGATCTGGCACCGCCCACCAAGAAATTG
  ATGATGTGGAAAGAGACAGGAGGAGTAGAAAAACTGTTTTCTTTACCTGCTCAGCCTTTG
  TGGAATAACAGACTACTGAAGCTCTTTACACGCTGTCTTACACCGCTTGTACCAGAAGAC
  CTTAGAAAAAGGAGGAAAGGAGGAGAGGCAGATAATTTGGATGAATTCCTCAAAGAATTT
  GAAAATCCAGAGGTTCCTAGAGAGGACCAGCAACAGCAGCATCAGCAGCGTGATGTTATC
  GATGAGCCCATTATTGAAGAGCCAAGCCGCCTCCAGGAGTCAGTGATGGAGGCCAGCAGA
  ACAAACATAGATGAGTCAGCTATGCCTCCACCACCACCTCAGGGAGTTAAGCGAAAAGCT
  GGACAAATTGACCCAGAGCCTGTGATGCCTCCTCAGCAGGTAGAGCAGATGGAAATACCA
  CCTGTAGAGCTTCCCCCAGAAGAACCTCCAAATATCTGTCAGCTAATACCAGAGTTAGAA
  CTTCTGCCAGAAAAAGAGAAGGAGAAAGAGAAGGAAAAAGAAGATGATGAAGAGGAAGAG
  GATGAAGATGCATCAGGGGGCGATCAAGATCAGGAAGAAAGAAGATGGAACAAAAGGACT
  CAGCAGATGCTTCATGGTCTTCAGCGTGCTCTTGCTAAAACTGGAGCTGAATCTATCAGT
  TTGCTTGAGTTATGTCGAAATACGAACAGAAAACAAGCTGCCGCAAAGTTCTACAGCTTC
  TTGGTTCTTAAAAAGCAGCAAGCTATTGAGCTGACACAGGAAGAACCGTACAGTGACATC
  ATCGCAACACCTGGACCAAGGTTCCATATTATATAA
  
  Clone variation with respect to NM_006265.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006265 unedited
  GTAACACGACTCTACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCGATTTGAACCGAG
  GATTTGGGCGGCAGGAAGAGCCGCGGCGTAACGGCAGCCATCTTGTTTGTTTGAGTGAAT
  CGGAAAGGAGGCGCCGGCTGTGGCGGCGGCGGGAGCTGCTCGGAAGCTACACCTCGCAAG
  GGCTCCCCCCTTTCCCCACCCCCTCCCCCGACCCTTTTCCCCTCCCCGGGCCACCCAGCC
  CGCCCAACTCCCAGCGGAGAGCAAGGTTTTCTTCTGTTTTCATAGCCAGCCAGAACAATG
  TTCTACGCACATTTTGTTCTCAGTAAAAGAGGGCCTCTGGCCAAAATTTGGCTAGCGGCC
  CATTGGGATAAGAAGCTAACCAAAGCCCATGTGTTCGAGTGTAATTTAGAGAGCAGCGTG
  GAGAGTATCATCTCACCAAAGGTGAAAATGGCATTACGGACATCAGGACATCTCTTACTG
  GGAGTAGTTCGAATCTATCACAGGAAAGCCAAATACCTTCTTGCAGACTGTAATGAAGCA
  TTCATTAAGATAAAGATGGCTTTTCGGCCAGGTGTGGTTGACCTGCCTGAGGAAAATCGG
  GAAGCAGCTTATAATGCCATTACTTTACCTGAAGAATTTCATGACTTTGATCAGCCACTG
  CCTGACTTAGATGACATCGATGTGGCCCAGCAGTTCAGCTTGAATCAGAGTAGAGTGGAA
  GAGATAACCATGAGAGAAGAAGTTGGGAACATCAGTATTTTACAAGAAATGATTNTGGTG
  ATTTGGNAATGGATGATCGTGAGATATGANAGAAGCAGTGCTTTTGAGATGACGACATGT
  ANTAGCACTACTACTTCTAACCTCTATANATCTGACAGACACCACATCTGATGAGAAATA
  C
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006265 unedited
  TTTTNNNNNNNNNNNNNNTTTTTTTTCTGTGNNANCNCGGNNCCGCATTTNANGATCGAG
  TTTTTTTTTTTTTTTTTTTTTTTTTTACAGCTTATAACACAACTTTTATTAGAAAAGTTA
  TACATAACATAGCATCAACTATTTTCAAGAACAATATTAAACCCGATAAGCAACAAAAAC
  CAGACTAACAAAATGTGTAACAAGAAACTAATGACCTTTCTAAAATCAAACATTCAATTA
  TCTACAATGTCTTTTTACAAACGGGGGAAAACTCCTTGGTTTACAGGCACATCATATTGA
  ATATANAAAGNCCTGGCAATAGCAATTTATACAATTACACTCTGAAGAACTGAATCATAA
  AACAGTAATACGAGTTCACAATTTAAAACATTTAACATAATTTTTAAATTATTGGGGGTT
  TCCCCTGGAAATTTGGGGTTTTAAAAAAAGGGGATTTTTTTTTTTCCCCCACAAGGTTAA
  AACTTTAAGCCAAAACTTTTGGGGGGAAACTTGCCCCCCAAAAAGGTGAAAAAAATTTTC
  TGGGAAAAGTAAACACCGCCCCCCGGATTTTTGGGGGGCTTTGGGGGGGGGGAAAAAAAA
  AAAAACCTTTTTTGGGGGGGAGGTTTTTTTTTCCCCCNNAAAAAACCGGCCCAAGAAGAC
  GGGGTTTTTTTGGGGGTTTTTTTTTCCCCCCCCCCCTTTTTTTTTTTTCCCCACCCACAA
  CGGGTGGGTTCCTCCTTGGGGGAAAGAAACCTCTCCCCCCCCCCCCCAAAAAAAAATATA
  TGAAAAAAGGAGCGTTTTTTTTTTCTCACCCCCCCCTTTTTTTTTTAAAAAAAAAAAAAG
  GGGCGGGGGGGGGGGGCCCCCCCCCCCCCACGCGGGGGCGGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006265
• Insert Size: 4000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006265.1, NP_006256.1
• RefSeq Size: 3647 bp
• RefSeq ORF: 1896 bp
• Locus ID: 5885
• Cytogenetics: 8q24.11
• Domains: Rad21_Rec8, Rad21_Rec8_N
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle
• Gene Summary: 'The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq, Jul 2008]'