MC5R (NM_005913) Human Untagged Clone

CAT#: SC116446

MC5R (untagged)-Human melanocortin 5 receptor (MC5R)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MC5R
• Synonyms: MC2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116446 sequence for NM_005913 edited (data generated by NextGen Sequencing)
  ATGAATTCCTCATTTCACCTGCATTTCTTGGATCTCAACCTGAATGCCACAGAGGGCAAC
  CTTTCAGGACCCAATGTCAAAAACAAGTCTTCACCATGTGAAGACATGGGCATTGCTGTG
  GAGGTGTTTCTCACTCTGGGTGTCATCAGCCTCTTGGAGAACATCTTGGTCATAGGGGCC
  ATAGTGAAGAACAAAAACCTGCACTCCCCCATGTACTTCTTCGTGTGCAGCCTGGCAGTG
  GCGGACATGCTGGTGAGCATGTCCAGTGCCTGGGAGACCATCACCATCTACCTACTCAAC
  AACAAGCACCTAGTGATAGCAGACGCCTTTGTGCGCCACATTGACAATGTGTTTGACTCC
  ATGATCTGCATTTCCGTGGTGGCATCCATGTGCAGCTTACTGGCCATTGCAGTGGATAGG
  TACGTCACCATCTTCTACGCCCTGCGCTACCACCACATCATGACGGCGAGGCGCTCAGGG
  GCCATCATCGCCGGCATCTGGGTTTTCTGCACGGGCTGCGGCATTGTCTTCATCCTGTAC
  TCAGAATCCACCTACGTCATCCTGTGCCTCATCTCCATGTTCTTCGCTATGCTGTTCCTC
  CTGGTGTCTCTGTACATACACATGTTCCTCCTGGCGCGGACTCACGTCAAGCGGATCGCG
  GCTCTGCCCGGGGCCAGCTCTGCGCGGCAGAGGACCAGCATGCAGGGCGCGGTCACCGTC
  ACCATGCTGCTGGGCGTGTTTACCGTGTGCTGGGCCCCGTTCTTCCTTCATCTCACTTTA
  ATGCTTTCTTGCCCTCAGAACCTCTACTGCTCTCGCTTCATGTCTCACTTCAATATGTAC
  CTCATACTCATCATGTGTAATTCCGTGATGGACCCTCTCATATATGCCTTCCGCAGCCAA
  GAGATGCGGAAGACCTTTAAGGAGATTATTTGCTGCCGTGGTTTCAGGATCGCCTGCAGC
  TTTCCCAGAAGGGATTAA
  
  Clone variation with respect to NM_005913.2
  503 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005913 unedited
  CGTTAGATTTGTATACGACTCATATAGGCGGCCGCGNAATCGGCACGAGGCAGCCGCCTA
  GCTGGGGGAGAGGCCCGTCCGGGGCTGGCTCTGAGCGCCGCACGGCCGTCGGATCCCGGG
  GACGCCGGGGGCCCGGGCTGAGCGCCGCGGCCCGCGAGGAGGAGCACCGTAAATTTGCTG
  CCAAGACAAGAGGTGTATTTCTCCAGCAATGAATTCCTCATTTCACCTGCATTTCTTGGA
  TCTCAACCTGAATGCCACAGAGGGCAACCTTTCAGGACCCAATGTCAAAAACAAGTCTTC
  ACCATGTGAAGACATGGGCATTGCTGTGGAGGTGTTTCTCACTCTGGGTGTCATCAGCCT
  CTTGGAGAACATCTTGGTCATAGGGGCCATAGTGAAGAACAAAAACCTGCACTCCCCCAT
  GTACTTCTTCGTGTGCAGCCTGGCAGTGGCGGACATGCTGGTGAGCATGTCCAGTGCCTG
  GGAGACCATCACCATCTACCTACTCAACAACAAGCACCTAGTGATAGCAGACGCCTTTGT
  GCGCCACATTGACAATGTGTTTGACTCCATGATCTGCATTTCCGTGGTGGCATCCATGTG
  CAGCTTACTGGCCATTGCAGTGGATAGGTACGTCACCATCTTCTACGCCCTGCGCTACCA
  CCACATCATGACGGCGAGGCGCTCAGGGGCCATCATCGCCGGCATCTGGGTTTTCTGCAC
  GGGCTGCGGCATTGNCTTCATCCTGTACTCAGAAGCCACCTACGTCATCCTGTGCCTCAT
  CTCCATGGTCTTCGCTATGCTGNTCCTCCTGGNGTCTCTGTACATACACATGTTCCTTCT
  GGCGCGGACTCACGTCAAGCGGATCGCTGCTCTGCCCTGGGCCAGCTCTGCGCTGCAGAG
  GAACAGCATGCAGGNCGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005913 unedited
  GGTGGGTGCAATCCCTTTTTATTTGTAGGTGTTATATCATTTTGGTCTGAGACTGGCCAT
  CCTACAGGCCATAAATATTGTCAAGGTAGACTTCACCACAAGTCACCAACGAATGTGCCT
  TCTCATTTTGCAGTAGCTGCTAACGAATTGGCAGAAAGACCAAAACCATTCTGGATATCA
  GAATGACCAGTTCAAACAGGTGGTCTGCTCCTTCCATTTTATTAACCATTTCCTTGAACA
  CACGTTTTTACATCAAACAAATTTCCTACAGAAGCAGATATTTAACATACCATATCATAC
  AATATTTGACAGTTATATGTATTCTTTTTGGGGGAGACACTTTGTGTTGGGTGCTAAGGG
  TTGGCTCATTTATGTACAGATATTTACCAAGTCACTACACACATGTATTCCTTCCAAATG
  AAGACACATCTAAATACTAGGGTTATTTCTGCACTATTTATAAAGATTAAAAGGCATTTG
  AGCATTTCCATAAAGACCTTTTTATGAAAGGTCAGATCTTGTAGCAACTCATGAAGCACC
  AGAGCAGAAAATTAGCCTAAAGAGAATTGGGTTGCTAGACGGGGCCAGTGTCAACCACAG
  GGTTGAAGATTCTGCCCATAGGACTGGAACTCATTTATTACTCAGGTCTACCAGGCCGAA
  GAGTCAATCACATTTGTTTTGCACCAATAACAAGCATTCTTGGTGCCCCTGCTCACTCCC
  TGAACTTGTTGTAAGACATGGGGCCACCTATTTGCTTGAGGCTAAGTAAAGCCAGTTTTA
  CAGAAGCCGTGTTCTGAGACACATCCCACGGAACAATACACTTAGGTTCCCACGGAGGTA
  CACGGAACCCTTTATGCCCTTTCGGGATCCCACCTGAATTTTCTCCATAGAGCCAGAAAC
  TTTGATAAAGAATTGGCCCTTAACTTAGTGGCACCACCGNACATCCCCCTTTAACAGTTT
  GGGTCTTAGCCCCCTCGTCATCTCACCCTACT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005913
• Insert Size: 4000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005913.1, NP_005904.1
• RefSeq Size: 978 bp
• RefSeq ORF: 978 bp
• Locus ID: 4161
• Cytogenetics: 18p11.21
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: 'This gene encodes a member of the seven-pass transmembrane G protein-coupled melanocortin receptor protein family that stimulate cAMP signal transduction. The encoded protein is a receptor for melanocyte-stimulating hormone and adrenocorticotropic hormone and is suggested to play a role in sebum generation. [provided by RefSeq, Jun 2010]'