EWSR1 (NM_005243) Human Untagged Clone

CAT#: SC116868

EWSR1 (untagged)-Human Ewing sarcoma breakpoint region 1 (EWSR1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EWSR1
• Synonyms: bK984G1.4; EWS; EWS-FLI1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005243, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGTCCACGGATTACAGTACCTATAGCCAAGCTGCAGCGCAGCAGGGCTACAGTGCTTACACCGCCC
  AGCCCACTCAAGGATATGCACAGACCACCCAGGCATATGGGCAACAAAGCTATGGAACCTATGGACAGCC
  CACTGATGTCAGCTATACCCAGGCTCAGACCACTGCAACCTATGGGCAGACCGCCTATGCAACTTCTTAT
  GGACAGCCTCCCACTGGTTATACTACTCCAACTGCCCCCCAGGCATACAGCCAGCCTGTCCAGGGGTATG
  GCACTGGTGCTTATGATACCACCACTGCTACAGTCACCACCACCCAGGCCTCCTATGCAGCTCAGTCTGC
  ATATGGCACTCAGCCTGCTTATCCAGCCTATGGGCAGCAGCCAGCAGCCACTGCACCTACAAGACCGCAG
  GATGGAAACAAGCCCACTGAGACTAGTCAACCTCAATCTAGCACAGGGGGTTACAACCAGCCCAGCCTAG
  GATATGGACAGAGTAACTACAGTTATCCCCAGGTACCTGGGAGCTACCCCATGCAGCCAGTCACTGCACC
  TCCATCCTACCCTCCTACCAGCTATTCCTCTACACAGCCGACTAGTTATGATCAGAGCAGTTACTCTCAG
  CAGAACACCTATGGGCAACCGAGCAGCTATGGACAGCAGAGTAGCTATGGTCAACAAAGCAGCTATGGGC
  AGCAGCCTCCCACTAGTTACCCACCCCAAACTGGATCCTACAGCCAAGCTCCAAGTCAATATAGCCAACA
  GAGCAGCAGCTACGGGCAGCAGAGTTCATTCCGACAGGACCACCCCAGTAGCATGGGTGTTTATGGGCAG
  GAGTCTGGAGGATTTTCCGGACCAGGAGAGAACCGGAGCATGAGTGGCCCTGATAACCGGGGCAGGGGAA
  GAGGGGGATTTGATCGTGGAGGCATGAGCAGAGGTGGGCGGGGAGGAGGACGCGGTGGAATGGGCAGCGC
  TGGAGAGCGAGGTGGCTTCAATAAGCCTGGTGGACCCATGGATGAAGGACCAGATCTTGATCTAGGCCCA
  CCTGTAGATCCAGATGAAGACTCTGACAACAGTGCAATTTATGTACAAGGATTAAATGACAGTGTGACTC
  TAGATGATCTGGCAGACTTCTTTAAGCAGTGTGGGGTTGTTAAGATGAACAAGAGAACTGGGCAACCCAT
  GATCCACATCTACCTGGACAAGGAAACAGGAAAGCCCAAAGGCGATGCCACAGTGTCCTATGAAGACCCA
  CCCACTGCCAAGGCTGCCGTGGAATGGTTTGATGGGAAAGATTTTCAAGGGAGCAAACTTAAAGTCTCCC
  TTGCTCGGAAGAAGCCTCCAATGAACAGTATGCGGGGTGGTCTGCCACCCCGTGAGGGCAGAGGCATGCC
  ACCACCACTCCGTGGAGGTCCAGGAGGCCCAGGAGGTCCTGGGGGACCCATGGGTCGCATGGGAGGCCGT
  GGAGGAGATAGAGGAGGCTTCCCTCCAAGAGGACCCCGGGGTTCCCGAGGGAACCCCTCTGGAGGAGGAA
  ACGTCCAGCACCGAGCTGGAGACTGGCAGTGTCCCAATCCGGGTTGTGGAAACCAGAACTTCGCCTGGAG
  AACAGAGTGCAACCAGTGTAAGGCCCCAAAGCCTGAAGGCTTCCTCCCGCCACCCTTTCCGCCCCCGGGT
  GGTGATCGTGGCAGAGGTGGCCCTGGTGGCATGCGGGGAGGAAGAGGTGGCCTCATGGATCGTGGTGGTC
  CCGGTGGAATGTTCAGAGGTGGCCGTGGTGGAGACAGAGGTGGCTTCCGTGGTGGCCGGGGCATGGACCG
  AGGTGGCTTTGGTGGAGGAAGACGAGGTGGCCCTGGGGGGCCCCCTGGACCTTTGATGGAACAGATGGGA
  GGAAGAAGAGGAGGACGTGGAGGACCTGGAAAAATGGATAAAGGCGAGCACCGTCAGGAGCGCAGAGATC
  GGCCCTACTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005243 unedited
  AGTCAGCATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGAAGGAGA
  GAAAATGGCGTCCACGGATTACAGTACCTATAGCCAAGCTGCAGCGCAGCAGGGCTACAG
  TGCTTACACCGCCCAGCCCACTCAAGGATATGCACAGACCACCCAGGCATATGGGCAACA
  AAGCTATGGAACCTATGGACAGCCCACTGATGTCAGCTATACCCAGGCTCAGACCACTGC
  AACCTATGGGCAGACCGCCTATGCAACTTCTTATGGACAGCCTCCCACTGGTTATACTAC
  TCCAACTGCCCCCCAGGCATACAGCCAGCCTGTCCAGGGGTATGGCACTGGTGCTTATGA
  TACCACCACTGCTACAGTCACCACCACCCAGGCCTCCTATGCAGCTCAGTCTGCATATGG
  CACTCAGCCTGCTTATCCAGCCTATGGGCAGCAGCCAGCAGCCACTGCACCTACAAGACC
  GCAGGATGGAAACAAGCCCACTGAGACTAGTCAACCTCAATCTAGCACAGGGGGTTACAA
  CCAGCCCAGCCTAGGATATGGACAGAGTAACTACAGTTATCCCCAGGTACCTGGGAGCTA
  CCCCATGCAGCCAGTCACTGCACCTCCATCCTACCCTCCTACCAGCTATTCCTCTACACA
  GCCGACTAGTTATGATCAGAGCAGTTACTCTCAGCAGAACACCTATGGGCAACCGAGCAG
  CTATGGACAGCAGAGTAGCTATGGTCAACAAAGCAGCTATGGGCAGCAGCCTTCCACTAG
  NTACCCACCCCCANACTGGATCCTACAGCCAAGCTNCAAGTCAATATAGCCCACAGAGCA
  GCAGCTACGGGCAGCAGAAGTCATCCCGACAGGACCACCCCATAGCATGGGTGTTTATGG
  GCAGGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005243 unedited
  CGGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTAACATTTGGAATTT
  TATTTAAAAAAAAAAAAAAAACATCACAACCATGGAACATTGTTACAGTTAAGAAGGCCC
  TCTTGGGTTCTCCACAATGATACTGGAGCATGCTCACAAGGGGCTTCCCATTGTTAAAGT
  CTTAAACAACCATTTTTAAAAGAAGGAAGAAAAAAAAACTCCGCACACTACCATTTAACT
  TGTTTTAATGTTTCTTCACAAATGGTGAAAAATACTAAAGTACAGACAAGGAATAATCAT
  AATGTTGGGGCCAACATTATAAATATGGAATTATAAATTTAAAACATTTTCTGGTTTAAA
  AAATAAATCTGGTAGTCAATGCAGCTCTGCGGGGTCTCTGCATCTAGTAGGGCCGATCTC
  TGCGCTCCTGACGGTGCTCGCCTTTATCCATTTTTCCAGGTCCTCCACGTCCTCCTCTTC
  TTCCTCCCATCTGTTCCATCAAAGGTCCAGGGGGCCCCCCAGGGCCACCTCGTCTTCCTC
  CACCAAAGCCACCTCGGTCCATGCCCCGGCCACCACGGAAGCCACCTCTGTCTCCACCAC
  GGCCACCTCTGAACATTCCACCGGGACCACCACGATCCATGAGGCCACCTCTTCCTCCCC
  GCATGCCACCAAGGCCACCTCTGCCACGATCACCACCCGGGGGCGGAAAGGTTGGCCGAA
  AGAAACCTTCAGCCTTTGGGGCCTTACACTGGTTGCACTCTGGTTCTCCAGCGAAGTTTT
  GGTTTCCACACCCCGTATTGGGACACTGCAGTCTCCAACTCGGGCTGGACGTTTCTTCCC
  AAAGGGTTCCCTCGGAACCCGGGGTCCTTTGAAGGGAACCTCCTTATTTCTTCAGCCTTC
  TTGCACCAGGTTCCCAAGACTCCGGGCTCCTGACTCCCGAAGGGTGGGCTGCCCTGCCTA
  CGGGGGCAAACCCCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005243
• Insert Size: 2330 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005243.1, NP_005234.1
• RefSeq Size: 2679 bp
• RefSeq ORF: 2679 bp
• Locus ID: 2130
• Cytogenetics: 22q12.2
• Domains: RRM, zf-RanBP
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Gene Summary: 'This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14. [provided by RefSeq, Jul 2009]'
  Transcript Variant: This variant (2) lacks an alternate in-frame exon and uses an alternate in-frame splice site in the coding region, compared to variant 1. The resulting isoform (2) is shorter than isoform 1.