RNASE4 (NM_002937) Human Untagged Clone

CAT#: SC118284

RNASE4 (untagged)-Human ribonuclease, RNase A family, 4 (RNASE4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RNASE4
• Synonyms: RAB1; RNS4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118284 sequence for NM_002937 edited (data generated by NextGen Sequencing)
  ATGGCTCTGCAGAGGACCCATTCATTGCTTCTGCTTTTGCTGCTGACCCTGCTGGGGCTG
  GGGCTGGTCCAGCCCTCCTATGGCCAGGATGGCATGTACCAGCGATTCCTGCGGCAACAC
  GTGCACCCTGAGGAGACAGGTGGCAGTGATCGCTACTGCAACTTGATGATGCAAAGACGG
  AAGATGACTTTGTATCACTGCAAGCGCTTCAACACCTTCATCCATGAAGATATCTGGAAC
  ATTCGTAGTATCTGCAGCACCACCAATATCCAATGCAAGAACGGCAAGATGAACTGCCAT
  GAGGGTGTAGTGAAGGTCACAGATTGCAGGGACACAGGAAGTTCCAGGGCACCCAACTGC
  AGATATCGGGCCATAGCGAGCACTAGACGTGTTGTCATTGCCTGTGAGGGTAACCCACAG
  GTGCCTGTGCACTTTGACGGTTAG
  
  Clone variation with respect to NM_002937.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002937 unedited
  TTTTGGTATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCCGTCCGTGTA
  CACACACTCACACAAGGACGCCAACCCCACCTAGATGCAAAGCAGGATTCAAAAGAACAT
  CTTTGCGTTTTCTACCGGCTCCCCATCATCGTACTAGGGAGGAAGAAGCGGGTGAGAAAC
  AAAACTTCTTTCCATTGTCCTGCCCGTTTCTGCGGACTTGTTCTGAGGCCGAGGCACCTC
  TAAGATACTGATGGCTCTGCAGAGGACCCATTCATTGCTTCTGCTTTTGCTGCTGACCCT
  GCTGGGGCTGGGGCTGGTCCAGCCCTCCTATGGCCAGGATGGCATGTACCAGCGATTCCT
  GCGGCAACACGTGCACCCTGAGGAGACAGGTGGCAGTGATCGCTACTGCAACTTGATGAT
  GCAAAGACGGAAGATGACTTTGTATCACTGCAAGCGCTTCAACACCTTCATCCATGAAGA
  TATCTGGAACATTCGTAGTATCTGCAGCACCACCAATATCCAATGCAAGAACGGCAAGAT
  GAACTGCCATGAGGGTGTAGTGAAGGTCACAGATTGCAGGGACACAGGAAGTTCCAGGGC
  ACCCAACTGCAGATATCGGGCCATAGCGAGCACTAGACGTGTTGTCATTGCCTGTGAGGG
  TAACCCACAGGTGCCTGTGCACTTTGACGGTTAGATGCCACCATGTANGGATTATCGCGA
  GTGGTTGACCCTACACTTACTCCTTAAATAGCAGNGAGTAATGCATTTGAGCTGTCCCAG
  CTCTGCCCCTCAGCTCTTTCCTACTCTTTTCTCTATATACTCCATCTATTAAAACATTGC
  ACCAAGGAGAATGGAGACATTAACCTGTAAGAATGAAGCTGGCCTTTTCTGTATAAGCTT
  CTTTTATATACTGCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002937 unedited
  CGCTCTCTAGAGTCGAGTTCTTCCCTTTTTTTTTTCTCAGCACCTTCGTTTCAGGCCCTT
  TATTACAAGGCAAAACATACAAAATCTTTCCATCAGCTAAGAGCACATAACCCCTTAATG
  ATCTACCACATTTTTTAGCACTTTGACTTACTAGGGCGATCCACAGCTCCAGGCAACAAG
  AACATCCCCAGCCTCATTCTCTTATTCTTATTAACCCCACACATAAGAATGCCCCAAAAT
  ACAGAGCTCACATTCTGCTGGCATACATTCCCAAACCCTCAATGCCCTCCCTGACAGGCG
  AATCCTAAGGATAAAAAAAGCACACACTTTCCAAACATCCCTTGCGATGAACCAGAAAAA
  CCCCTGCATCAGCGGCCACCTCCCCTGGATCTTTGCCCCAATCCTTCCATCACCTTTACA
  GTCTTCACCACACCCCTCACCTCCGAGCTTCTATATGCACCGCCCTCATCATAAGCGCTG
  CGGCCCCAACATGCGATACCCATACATGAAAGCCTCCCGAAATGCCCGAAATACCACATA
  CCTCCCCATACTCCCCACCTCCCACAGGATAGGATCTGACAGAGCTAACCTCGCCGTCCC
  ATCCAAGGAAGCCCATTACAGAACAGCCCACCCTCTCTCTCCCAGGCCTATGCCCCCATA
  TCCCTCTGGCGCCTGCCCCCAACCCATGAGCCATCTCCAGAAAAGAGCCCGCAATGACCT
  GAGGACACACACCCTGGCACAGCCCCAAATGCATACTCCCTGCCATCTCAGGACCAGCGC
  CAAGCCACCCCCTCCCCATACCCCTACCGGGGCCTCCCACCGCCCAAGTCCCCAGCCCCT
  GTGGCCACCCTCCAGCCCAGCCCCCCCCCCAGTGCCCCCTATGCCCCATTCTGCACCTGC
  TCCCCTGCACTNCCTGGCCCCTGCATCTGGCCCTCTCTACCCCCTCTGGCGCCCATCTGC
  CCGTCTGCATGCAATTGGCGGGGCCCCAACCTCCACGCTCCCATCTCCTGTAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002937
• Insert Size: 1430 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002937.3, NP_002928.1
• RefSeq Size: 1805 bp
• RefSeq ORF: 444 bp
• Locus ID: 6038
• Cytogenetics: 14q11.2
• Domains: RNAse_Pc
• Protein Families: Secreted Protein, Transmembrane
• Gene Summary: 'The protein encoded by this gene belongs to the pancreatic ribonuclease family. It plays an important role in mRNA cleavage and has marked specificity towards the 3' side of uridine nucleotides. Alternative splicing results in four transcript variants encoding the same protein. This gene and the gene that encodes angiogenin share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region. [provided by RefSeq, Aug 2013]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 4. All four variants encode the same protein.