ATX2 (ATXN2) (NM_002973) Human Untagged Clone

CAT#: SC118310

ATXN2 (untagged)-Human ataxin 2 (ATXN2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATXN2
• Synonyms: ATX2; SCA2; TNRC13
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002973, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCGCTCAGCGGCCGCAGCTCCTCGGAGTCCCGCGGTGGCCACCGAGTCTCGCCGCTTCGCCGCAGCCA
  GGTGGCCCGGGTGGCGCTCGCTCCAGCGGCCGGCGCGGCGGAGCGGGCGGGGCGGCGGTGGCGCGGCCCC
  GGGACCGTATCCCTCCGCCGCCCCTCCCCCGCCCGGCCCCGGCCCCCCTCCCTCCCGGCAGAGCTCGCCT
  CCCTCCGCCTCAGACTGTTTTGGTAGCAACGGCAACGGCGGCGGCGCGTTTCGGCCCGGCTCCCGGCGGC
  TCCTTGGTCTCGGCGGGCCTCCCCGCCCCTTCGTCGTCCTCCTTCTCCCCCTCGCCAGCCCGGGCGCCCC
  TCCGGCCGCGCCAACCCGCGCCTCCCCGCTCGGCGCCCGCGCGTCCCCGCCGCGTTCCGGCGTCTCCTTG
  GCGCGCCCGGCTCCCGGCTGTCCCCGCCCGGCGTGCGAGCCGGTGTATGGGCCCCTCACCATGTCGCTGA
  AGCCCCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAGCAGCAGCAGCA
  GCAGCCGCCGCCCGCGGCTGCCAATGTCCGCAAGCCCGGCGGCAGCGGCCTTCTAGCGTCGCCCGCCGCC
  GCGCCTTCGCCGTCCTCGTCCTCGGTCTCCTCGTCCTCGGCCACGGCTCCCTCCTCGGTGGTCGCGGCGA
  CCTCCGGCGGCGGGAGGCCCGGCCTGGGCAGAGGTCGAAACAGTAACAAAGGACTGCCTCAGTCTACGAT
  TTCTTTTGATGGAATCTATGCAAATATGAGGATGGTTCATATACTTACATCAGTTGTTGGCTCCAAATGT
  GAAGTACAAGTGAAAAATGGAGGTATATATGAAGGAGTTTTTAAAACTTACAGTCCGAAGTGTGATTTGG
  TACTTGATGCCGCACATGAGAAAAGTACAGAATCCAGTTCGGGGCCGAAACGTGAAGAAATAATGGAGAG
  TATTTTGTTCAAATGTTCAGACTTTGTTGTGGTACAGTTTAAAGATATGGACTCCAGTTATGCAAAAAGA
  GATGCTTTTACTGACTCTGCTATCAGTGCTAAAGTGAATGGCGAACACAAAGAGAAGGACCTGGAGCCCT
  GGGATGCAGGTGAACTCACAGCCAATGAGGAACTTGAGGCTTTGGAAAATGACGTATCTAATGGATGGGA
  TCCCAATGATATGTTTCGATATAATGAAGAAAATTATGGTGTAGTGTCTACGTATGATAGCAGTTTATCT
  TCGTATACAGTGCCCTTAGAAAGAGATAACTCAGAAGAATTTTTAAAACGGGAAGCAAGGGCAAACCAGT
  TAGCAGAAGAAATTGAGTCAAGTGCCCAGTACAAAGCTCGAGTGGCCCTGGAAAATGATGATAGGAGTGA
  GGAAGAAAAATACACAGCAGTTCAGAGAAATTCCAGTGAACGTGAGGGGCACAGCATAAACACTAGGGAA
  AATAAATATATTCCTCCTGGACAAAGAAATAGAGAAGTCATATCCTGGGGAAGTGGGAGACAGAATTCAC
  CGCGTATGGGCCAGCCTGGATCGGGCTCCATGCCATCAAGATCCACTTCTCACACTTCAGATTTCAACCC
  GAATTCTGGTTCAGACCAAAGAGTAGTTAATGGAGGTGTTCCCTGGCCATCGCCTTGCCCATCTCCTTCC
  TCTCGCCCACCTTCTCGCTACCAGTCAGGTCCCAACTCTCTTCCACCTCGGGCAGCCACCCCTACACGGC
  CGCCCTCCAGGCCCCCCTCGCGGCCATCCAGACCCCCGTCTCACCCCTCTGCTCATGGTTCTCCAGCTCC
  TGTCTCTACTATGCCTAAACGCATGTCTTCAGAAGGGCCTCCAAGGATGTCCCCAAAGGCCCAGCGACAT
  CCTCGAAATCACAGAGTTTCTGCTGGGAGGGGTTCCATATCCAGTGGCCTAGAATTTGTATCCCACAACC
  CACCCAGTGAAGCAGCTACTCCTCCAGTAGCAAGGACCAGTCCCTCGGGGGGAACGTGGTCATCAGTGGT
  CAGTGGGGTTCCAAGATTATCCCCTAAAACTCATAGACCCAGGTCTCCCAGACAGAACAGTATTGGAAAT
  ACCCCCAGTGGGCCAGTTCTTGCTTCTCCCCAAGCTGGTATTATTCCAACTGAAGCTGTTGCCATGCCTA
  TTCCAGCTGCATCTCCTACGCCTGCTAGTCCTGCATCGAACAGAGCTGTTACCCCTTCTAGTGAGGCTAA
  AGATTCCAGGCTTCAAGATCAGAGGCAGAACTCTCCTGCAGGGAATAAAGAAAATATTAAACCCAATGAA
  ACATCACCTAGCTTCTCAAAAGCTGAAAACAAAGGTATATCACCAGTTGTTTCTGAACATAGAAAACAGA
  TTGATGATTTAAAGAAATTTAAGAATGATTTTAGGTTACAGCCAAGTTCTACTTCTGAATCTATGGATCA
  ACTACTAAACAAAAATAGAGAGGGAGAAAAATCAAGAGATTTGATCAAAGACAAAATTGAACCAAGTGCT
  AAGGATTCTTTCATTGAAAATAGCAGCAGCAACTGTACCAGTGGCAGCAGCAAGCCGAATAGCCCCAGCA
  TTTCCCCTTCAATACTTAGTAACACGGAGCACAAGAGGGGACCTGAGGTCACTTCCCAAGGGGTTCAGAC
  TTCCAGCCCAGCATGTAAACAAGAGAAAGACGATAAGGAAGAGAAGAAAGACGCAGCTGAGCAAGTTAGG
  AAATCAACATTGAATCCCAATGCAAAGGAGTTCAACCCACGTTCCTTCTCTCAGCCAAAGCCTTCTACTA
  CCCCAACTTCACCTCGGCCTCAAGCACAACCTAGCCCATCTATGGTGGGTCATCAACAGCCAACTCCAGT
  TTATACTCAGCCTGTTTGTTTTGCACCAAATATGATGTATCCAGTCCCAGTGAGCCCAGGCGTGCAACCT
  TTATACCCAATACCTATGACGCCCATGCCAGTGAATCAAGCCAAGACATATAGAGCAGTACCAAATATGC
  CCCAACAGCGGCAAGACCAGCATCATCAGAGTGCCATGATGCACCCAGCGTCAGCAGCGGGCCCACCGAT
  TGCAGCCACCCCACCAGCTTACTCCACGCAATATGTTGCCTACAGTCCTCAGCAGTTCCCAAATCAGCCC
  CTTGTTCAGCATGTGCCACATTATCAGTCTCAGCATCCTCATGTCTATAGTCCTGTAATACAGGGTAATG
  CTAGAATGATGGCACCACCAACACACGCCCAGCCTGGTTTAGTATCTTCTTCAGCAACTCAGTACGGGGC
  TCATGAGCAGACGCATGCGATGTATGCATGTCCCAAATTACCATACAACAAGGAGACAAGCCCTTCTTTC
  TACTTTGCCATTTCCACGGGCTCCCTTGCTCAGCAGTATGCGCACCCTAACGCTACCCTGCACCCACATA
  CTCCACACCCTCAGCCTTCAGCTACCCCCACTGGACAGCAGCAAAGCCAACATGGTGGAAGTCATCCTGC
  ACCCAGTCCTGTTCAGCACCATCAGCACCAGGCCGCCCAGGCTCTCCATCTGGCCAGTCCACAGCAGCAG
  TCAGCCATTTACCACGCGGGGCTTGCGCCAACTCCACCCTCCATGACACCTGCCTCCAACACGCAGTCGC
  CACAGAATAGTTTCCCAGCAGCACAACAGACTGTCTTTACGATCCATCCTTCTCACGTTCAGCCGGCGTA
  TACCAACCCACCCCACATGGCCCACGTACCTCAGGCTCATGTACAGTCAGGAATGGTTCCTTCTCATCCA
  ACTGCCCATGCGCCAATGATGCTAATGACGACACAGCCACCCGGCGGTCCCCAGGCCGCCCTCGCTCAAA
  GTGCACTACAGCCCATTCCAGTCTCGACAACAGCGCATTTCCCCTATATGACGCACCCTTCAGTACAAGC
  CCACCACCAACAGCAGTTGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002973 unedited
  GGCCCACCCNCCTTTATCCCNNNGGTTAGTATTTGTATACNACTCTTATAGGCGGCCGCG
  NAATTCGCACGAGCTCCGATGCGCTCAGCGGCCGCAGCTCCTCGGAGTCCCGCGGTGGCC
  ACCGAGTCTCGCCGCTTCGCCGCAGCCAGGTGGCCCGGGTGGCGCTCGCTCCAGCGGCCG
  GCGCGGCGGAGCGGGCGGGGCGGCGGTGGCGCGGCCCCGGGACCGTATCCCTCCGCCGCC
  CCTCCCCCGCCCGGCCCCGGCCCCCCTCCCTCCCGGCAGAGCTCGCCTCCCTCCGCCTCA
  GACTGTTTTGGTAGCAACGGCAACGGCGGCGGCGCGTTTCGGCCCGGCTCCCGGCGGCTC
  CTTGGTCTCGGCGGGCCTCCCCGCCCCTTCGTCGTACTCCTTCTCCCCCTCGCCAGCCCG
  GGCGCCCCTCCGGCCGCGCCAACCCGCGCCTACCCGCTCGGCGCCCGCGCGTTCCCGCCG
  CGTTCCGGCGTCTCCTTGGCGCGCCCGGCTTCCCGACTGTACCCGCCCCGCGTGCGAGCC
  CGTGTATGGGCCCCTCACCATGTCGCTGAATCCCAGCACCCACCTCATCAGCAGCATCAA
  CAGTCTCAGCAGCAACAGCAGCAGCAGCAGAAGCATTAGCCGACGCCCGCGGCTGCCAAT
  GTCCCAAGCCCTGCCGGAACGGCCTTTTAGCGTGGCCCGCCGCCGGGCCTTTCGCGGCCC
  TCGGACTCGGGCTTCTCGGTCTCGGGCCACGGATCCCTTCTTNGGTGGTCGCGGGGACCT
  CGGGCGGCGGGGAGGCCCCGTCTGGGCCGAGGTCCAAAACATAACAAGGAATCGCCTCAA
  TCCACCACTTCTTTTTGAGGGAAATTTATCCACATTGAGGGATGGTTCTATCCTCCCACC
  CACTTTGGCCCCCACTTGTAAATTCACATTGAACATGGAGGTATATTTACAGCGTTTTTT
  AAACTTCACGCCCTAAGGGGTGATTGCTCCTTGCAGTTCTAAATAAAAAAATCCCCAATT
  CCTCTTGTGGCCTTAATCTCAAAATA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002973 unedited
  NAAGCGCTACTAGGTACCTGGCCCCCCTCGATGTCGAGTTTTTTTTTTTTTTTTTTTTAG
  CAGTAATATCAGCAAGAATCACTCTTGTTACTTCTTTTGCTAGCTGATGTGTTCATGACT
  TTCAAGGGTTATTAAAAAATAAATAACTTCCAGTTTCGGCAAGCAGAGCTGGGGTACCTG
  CGGGACTCTGAAACAGCATATGGAATTATGGAATAGCCCCCAAGTTCCTAAATGCCTCTA
  CTCGGTCCAAGTATCTTCCACTGCAAGTGAACTGTTAGCATTCCTATTGGATGTTACAAG
  AAATCAACATATATATTTTAAAAACAAAATAAATGAAATTCTAGTTTTCTGTGCTTCCAG
  TTGGTAGAAGCAGTAGAAGGGAGGAGGGAATTTGGCCTTTCGGTTCCTCCAGGGCAGCCT
  TACAACTGCTGTTGGTGGTGGGCTTGTACTGAAGGGTGCGTCATATAGGGGAAATGCGCT
  GTTGTCGAGACTGGAATGGGCTGTAGTGCACTTTGAGCGAGGGCGGCCTGGGGACCGCCG
  GGTGGCTGTGTCGTCATTAGCATCATTGGCGCATGGGCAGTTGGATGAGAAGGAACCATT
  CCTGACTGTACATGAGCCTGAGGTACGTGGGCCATGTGGGGTGGGTTGGTATACGCCGGC
  TGAACGTGAGAAGGATGGATCGTAAAGACAGTCTGTTGTGCTGCTGGGAAACTATTCTGT
  GGCGACTGCGTGTTGGAGGCAGGTGTCATGGAGGGGGGAGTTGGCGCAAGCCCCGCGTGG
  TAAATGGCTGACTGCTGCTGTGGACTGGCCANATGGAGAGCCTGGGCGCCCTGGTGCTGA
  TGGTGCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002973
• Insert Size: 4500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002973.2, NP_002964.2
• RefSeq Size: 4712 bp
• RefSeq ORF: 3942 bp
• Locus ID: 6311
• Cytogenetics: 12q24.12
• Gene Summary: 'This gene belongs to a group of genes that is associated with microsatellite-expansion diseases, a class of neurological and neuromuscular disorders caused by expansion of short stretches of repetitive DNA. The protein encoded by this gene has two globular domains near the N-terminus, one of which contains a clathrin-mediated trans-Golgi signal and an endoplasmic reticulum exit signal. The encoded cytoplasmic protein localizes to the endoplasmic reticulum and plasma membrane, is involved in endocytosis, and modulates mTOR signals, modifying ribosomal translation and mitochondrial function. The N-terminal region of the protein contains a polyglutamine tract of 14-31 residues that can be expanded in the pathogenic state to 32-200 residues. Intermediate length expansions of this tract increase susceptibility to amyotrophic lateral sclerosis, while long expansions of this tract result in spinocerebellar ataxia-2, an autosomal-dominantly inherited, neurodegenerative disorder. Genome-wide association studies indicate that loss-of-function mutations in this gene may be associated with susceptibility to type I diabetes, obesity and hypertension. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2016]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.