MCC (NM_002387) Human Untagged Clone

CAT#: SC118657

MCC (untagged)-Human mutated in colorectal cancers (MCC), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MCC
• Synonyms: MCC1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002387, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAATTCCGGAGTTGCCATGAAATATGGAAACGACTCCTCGGCCGAGCTGAGTGAGCTCCATTCAGCAG
  CCCTGGCATCACTAAAGGGAGATATAGTGGAACTTAATAAACGTCTCCAGCAAACAGAGAGGGAACGGGA
  CCTTCTGGAAAAGAAATTGGCCAAGGCACAGTGCGAGCAGTCCCACCTCATGAGAGAGCATGAGGATGTC
  CAGGAGCGAACGACACTTCGCTATGAGGAACGCATCACAGAGCTCCACAGCGTCATTGCGGAGCTCAACA
  AGAAGATAGACCGTCTGCAAGGCACCACCATCAGGGAGGAAGATGAGTACTCAGAACTGCGATCAGAACT
  CAGCCAGAGCCAACACGAGGTCAACGAGGACTCTCGAAGCATGGACCAAGACCAGACCTCTGTCTCTATC
  CCCGAAAACCAGTCTACCATGGTTACTGCTGACATGGACAACTGCAGTGACCTGAACTCAGAACTGCAGA
  GGGTGCTGACAGGGCTGGAGAATGTTGTCTGCGGCAGGAAGAAGAGCAGCTGCAGCCTCTCCGTGGCCGA
  GGTGGACAGGCACATTGAGCAGCTCACCACAGCCAGCGAGCACTGTGACCTGGCTATTAAGACAGTCGAG
  GAGATTGAGGGGGTGCTTGGCCGGGACCTGTATCCCAACCTGGCTGAAGAGAGGTCTCGGTGGGAGAAGG
  AGCTGGCTGGGCTGAGGGAAGAGAATGAGAGCCTGACTGCCATGCTGTGCAGCAAAGAGGAAGAACTGAA
  CCGGACTAAGGCCACCATGAATGCCATCCGGGAAGAGCGGGACCGGCTCCGGAGGAGGGTCAGAGAGCTT
  CAAACTCGACTACAGAGCGTGCAGGCCACAGGTCCCTCCAGCCCTGGCCGCCTCACTTCCACCAACCGCC
  CGATTAACCCCAGCACTGGGGAGCTGAGCACAAGCAGCAGCAGCAATGACATTCCCATCGCCAAGATTGC
  TGAGAGGGTGAAGCTATCAAAGACAAGGTCCGAATCGTCATCATCTGATCGGCCAGTCCTGGGCTCAGAA
  ATCAGTAGCATAGGGGTATCCAGCAGTGTGGCTGAACACCTGGCCCACTCACTTCAGGACTGCTCCAATA
  TCCAAGAGATTTTCCAAACACTCTACTCACACGGATCTGCCATCTCAGAAAGCAAGATTAGAGAGTTTGA
  GGTGGAAACAGAACGGCTGAATAGCCGGATTGAGCACCTCAAATCCCAAAATGACCTCCTGACCATAACC
  TTGGAGGAATGTAAAAGCAATGCCGAGAGGATGAGCATGCTGGTGGGAAAATACGAATCCAATGCCACAG
  CGCTGAGGCTGGCCTTGCAGTACAGCGAGCAGTGCATCGAAGCCTACGAACTCCTCCTGGCGCTGGCAGA
  GAGTGAGCAGAGCCTCATCCTGGGGCAGTTCCGAGCGGCGGGCGTGGGGTCCTCCCCTGGAGACCAGTCG
  GGGGATGAAAACATCACTCAGATGCTCAAGCGAGCTCATGACTGCCGGAAGACAGCTGAGAACGCTGCCA
  AGGCCCTGCTCATGAAGCTGGACGGCAGCTGTGGGGGAGCCTTTGCCGTGGCCGGCTGCAGCGTGCAGCC
  CTGGGAGAGCCTTTCCTCCAACAGCCACACCAGCACAACCAGCTCCACAGCCAGTAGTTGCGACACCGAG
  TTCACTAAAGAAGACGAGCAGAGGCTGAAGGATTATATCCAGCAGCTCAAGAATGACAGGGCTGCGGTCA
  AGCTGACCATGCTGGAGCTGGAAAGCATCCACATCGATCCTCTCAGCTATGACGTCAAGCCTCGGGGAGA
  CAGCCAGAGGCTGGATCTGGAAAACGCAGTGCTTATGCAGGAGCTCATGGCCATGAAGGAGGAGATGGCC
  GAGTTGAAGGCCCAGCTCTACCTACTGGAGAAAGAGAAGAAGGCCCTGGAGCTGAAGCTGAGCACGCGGG
  AGGCCCAGGAGCAGGCCTACCTGGTGCACATTGAGCACCTGAAGTCCGAGGTGGAGGAGCAGAAGGAGCA
  GCGGATGCGATCCCTCAGCTCCACCAGCAGCGGCAGCAAAGACAAACCTGGCAAGGAGTGTGCTGATGCT
  GCCTCCCCAGCTCTGTCCCTAGCCGAACTCAGGACAACGTGCAGCGAGAATGAGCTGGCTGCGGAGTTCA
  CCAACGCCATTCGTCGAGAAAAGAAGTTGAAGGCCAGAGTTCAAGAGCTGGTGAGTGCCTTGGAGAGACT
  CACCAAGAGCAGTGAAATCCGACATCAGCAATCTGCAGAGTTCGTGAATGATCTAAAGCGGGCCAACAGC
  AACCTGGTGGCTGCCTATGAGAAAGCAAAGAAAAAGCATCAAAACAAACTGAAGAAGTTAGAGTCGCAGA
  TGATGGCCATGGTGGAGAGACATGAGACCCAAGTGAGGATGCTCAAGCAAAGAATAGCTCTGCTAGAGGA
  GGAGAACTCCAGGCCACACACCAATGAAACTTCGCTTTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002387 unedited
  TTCGGCACGAGGCAGCACCCTCAGATCTGCCTAACTGCGTTGAACAGAGACGAGAGAACA
  GAGACTTGATGAAATGAGCATCAGCTCCATTCAGCAGCCCTGGCATCACTAAAGGGAGAT
  ATAGTGGAACTTAATAAACGTCTCCAGCAAACAGAGAGGGAACGGGACCTTCTGGAAAAG
  AAATTGGCCAAGGCACAGTGCGAGCAGTCCCACCTCATGAGAGAGCATGAGGATGTCCAG
  GAGCGAACGACGCTTCGCTATGAGGAACGCATCACAGAGCTCCACAGCGTCATTGCGGAG
  CTCAACAAGAAGATAGACCGTCTGCAAGGCACCACCATCAGGGAGGAAGATGAGTACTCA
  GAACTGCGATCAGAACTCAGCCAGAGCCAACACGAGGTCAACGAGGACTCTCGAAGCATG
  GACCAAGACCAGACCTCTGTCTCTATCCCCGAAAACCAGTCTACCATGGTTACTGCTGAC
  ATGGACAACTGCAGTGACCTGAACTCANAACTGCANAGGGTGCTGACAGGGCTGGAGAAT
  GTTGTCTGCGGCAGGAAGAAGAGCAGCTGCAGCCTCTCCGTGGCCGNGGTGGACAGGCAC
  ATTGAGCAGCTCACCACAGCCAGCGAGCACTGTGACCTGGCTATTAANACAGTCNANGAG
  ATTGAGGGGGTGCTTGGNCGGNACCTGTATTCCCACCTGGCTGANNANAGGTNNTCGTGG
  GNAAAAAGAGCTGCTGGNCTGAGGGAANAAANTGAGAGNCTGACTGCCATGCTGTGCAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002387 unedited
  CAATCCTTGCCCGCGGCCGCAATTTAGATNCGGTTTTTTTTTTTTTTTTTGGGGCATATG
  CTTAAATAGTCCAAACATTTTCCTCATGATAAACATGCAACTTTATATGAAAAGTGAAAA
  ATTCTAAAAAATCACAGGAATGGCCAGGCACATTGGCTCATGCCTGTAATCCCAGCACTT
  TGGGAGGCCGAGGTGGGTGGATCAGTGGTCAGGAGTTCTATATTTTCTGGCCAACATGGT
  GAAACCCTGTCTTTACTAAAAATACAAAAAAACTAGCTGGGTATGGTGGCGGGCACCTGT
  AATTCCAGCTACTCAGGAGGCAGAGGTTGCAGTGAGCCAAGATCACGCCACTGCACTTCA
  GCCTGGTGACAAGAGCAAAACTTCATTTTAAAAAAAAAAAAAAAAAAAATCACAGGAACT
  AACTTTGACCAAGTGATTGACAGAAACCAGTGAACATTTTTATAGTGATCAAAGCACCCC
  AAAGCTGGTCCTTCTAGACTGATACCAGCTGATCTGTTGTTTCGTTTTGGAGATGGGCAG
  CCTCACTGCTGGCCTCTCAGCGGTTGCTTTTAGGATACAGCTGTCTGCCCTCCGAACTGG
  TGGCTGGAGTATCCTTCAATGAACAAAAGGCTGTGGAAATTGTATCATGCTTTGAAAAAG
  ACCTACCCGTAGCTTATGGACATAAAGACTTTATGTCGAAACCTTCTCCACCTTTTAATT
  TGTAGTCAGATATGGCAATGGAACACATTTTTCATAGGGAACTTTGCATGGAATTTCTTG
  GTGTTACACACAAAAACAGTAAAGGGGCCCTTTAAGTGTATCCACTGTACACCTGAAAGA
  GAAAAGGTAAAACTTACAAACAATATGAACTTTGTTATAGAATGAAAACTCTCCTGGACA
  CACCAATTCCCCTGGGACTGCCAACCCTGTAACCATTTCCCCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002387
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002387.1, NP_002378.1
• RefSeq Size: 4181 bp
• RefSeq ORF: 2490 bp
• Locus ID: 4163
• Cytogenetics: 5q22.2
• Protein Families: Druggable Genome
• Gene Summary: 'This gene is a candidate colorectal tumor suppressor gene that is thought to negatively regulate cell cycle progression. The orthologous gene in the mouse expresses a phosphoprotein associated with the plasma membrane and membrane organelles, and overexpression of the mouse protein inhibits entry into S phase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) has a distinct 5' UTR and 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.