Dermatopontin (DPT) (NM_001937) Human Untagged Clone

CAT#: SC118957

DPT (untagged)-Human dermatopontin (DPT)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DPT
• Synonyms: TRAMP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118957 sequence for NM_001937 edited (data generated by NextGen Sequencing)
  ATGGACCTCAGTCTTCTCTGGGTACTTCTGCCCCTAGTCACCATGGCCTGGGGCCAGTAT
  GGCGATTATGGATACCCATACCAGCAGTATCATGACTACAGCGATGATGGGTGGGTGAAT
  TTGAACCGGCAAGGCTTCAGCTACCAGTGTCCCCAGGGGCAGGTGATAGTGGCCGTGAGG
  AGCATCTTCAGCAAGAAGGAAGGTTCTGACAGACAATGGAACTACGCCTGCATGCCCACA
  CCACAGAGCCTCGGGGAACCCACGGAGTGCTGGTGGGAGGAGATCAACAGGGCTGGCATG
  GAATGGTACCAGACGTGCTCCAACAATGGGCTGGTGGCAGGATTCCAGAGCCGCTACTTC
  GAGTCAGTGCTGGATCGGGAGTGGCAGTTTTACTGTTGTCGCTACAGCAAGAGGTGCCCA
  TATTCCTGCTGGCTAACAACAGAATATCCAGGTCACTATGGTGAGGAAATGGACATGATT
  TCCTACAATTATGATTACTATATCCGAGGAGCAACAACCACTTTCTCTGCAGTGGAAAGG
  GATCGCCAGTGGAAGTTCATAATGTGCCGGATGACTGAATACGACTGTGAATTTGCAAAT
  GTTTAG
  
  Clone variation with respect to NM_001937.4
  240 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001937 unedited
  GTAATACGCACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGATCCCAGGCAGCATGG
  ACCTCAGTCTTCTCTGGGTACTTCTGCCCCTAGTCACCATGGCCTGGGGCCAGTATGGCG
  ATTATGGATACCCATACCAGCAGTATCATGACTACAGCGATGATGGGTGGGTGAATTTGA
  ACCGGCAAGGCTTCAGCTACCAGTGTCCCCAGGGGCAGGTGATAGTGGCCGTGAGGAGCA
  TCTTCAGCAAGAAGGAAGGTTCTGACAGACAATGGAACTACGCCTGCATGCCCACACCAC
  AGAGCCTCGGGGAACCCACGGAGTGCTGGTGGGAGGAGATCAACAGGGCTGGCATGGAAT
  GGTACCAGACGTGCTCCAACAATGGGCTGGTGGCAGGATTCCAGAGCCGCTACTTCGAGT
  CAGTGCTGGATCGGGAGTGGCAGTTTTACTGTTGTCGCTACAGCAAGAGGTGCCCATATT
  CCTGCTGGCTAACAACAGAATATCCAGGTCACTATGGTGAGGAAATGGACATGATTTCCT
  ACAATTATGATTACTATATCCGAGGAGCAACAACCACTTTCTCTGCAGTGGAAAGGGATC
  GCCAGTGGAAGTTCATAATGTGCCGGATGACTGAATACGACTGTGAATTTGCAATGTTTA
  GATTTGCACATACCAAATCTGGGTGAAAGGAAGGGGCCGGTGACAGGAGGGTGTCCACAT
  ATGTTAACATCAGTTGGATCTCCTATAGAAGTTTCTGCTGCTCTCTTTCCTTCCTCCTGA
  GCTGGTAACTGCAATGCCAACTTTCCTGGCCCTTTCTGACTGGAATACACTTNTAATAAA
  ATCCCCATTAAACCATGAAAAAAAAAAAAAAAACTTGACTTTAGATTGCGCCCGCGTCAT
  AGCTGTTTCTGAAACAATCCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001937 unedited
  TCTATGGACCGCGGCACGCAATCTAGATCGAGTTTTTTTTTTTTTTTTTTAATGGCTTAA
  TTGTGGATTTTATTAGAAGTGTGATACTAGTCAGAAGGCCCAGGAAGTTGGCATTGCAGT
  TACCAGCTCAGGGAGAAGGAAAGAGAGCAGCAGAAACTTCTATAGGAGATCCAACTGATG
  TTAACATATGTGGACACCCTCCTGTCCCCGGCCCCTTTCCTTTCACCCAGATTTGGTATG
  TGGCAAATCTAAACATTTGCAAATTCACAGTCGTATTCAGTCATCCGGCACATTATGAAC
  TTCCACTGGCGATCCCTTTCCACTGCAGAGAAAGTGGTTGTTGCTCCTCGGATATAGTAA
  TCATAATTGTAGGAAATCATGTCCATTTCCTCACCATAGTGACCTGGATATTCTGTTGTT
  AGCCAGCAGGAATATGGGCACCTCTTGCTGTAGCGACAACAGTAAAACTGCCACTCCCGA
  TCCAGCACTGACTCGAAGTAGCGGCTCTGGAATCCTGCCACCAGCCCATTGTTGGAGCAC
  GTCTGGTACCATTCCATGCCAGCCCTGTTGATCTCCTCCCACCAGCACTCCGTGGGTTCC
  CCGAGGCTCTGTGGTGTGGGCATGCAGGCGTAGTTCCATTGTCTGTCAGAACCTTCCTTC
  TTGCTGAAGATGCTCCTCACGGCCACTATCACCTGCCCCTGGGGACACTGGTAGCTGAAG
  CCTTGCCGGTTCAAATTCACCCACCCATCATCGCTGTAGTCATGATACTGCTGGTATGGN
  TATCCATAATCGCCATACTGGCCCCCAGCCATGGTGACTAGGGGCAGAAGTACCCCAGAG
  AGACTGAGGTCCATGCTGCCTGGGATCCTCGTGCCAATNCGCGGCCGCCCTAAGGGAGTC
  GTATTACAAATCTGACGGTTACTAACGAGCTCTGCTATTAGACCTCCACGCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001937
• Insert Size: 870 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001937.3, NP_001928.2
• RefSeq Size: 1735 bp
• RefSeq ORF: 606 bp
• Locus ID: 1805
• Cytogenetics: 1q24.2
• Protein Families: Secreted Protein
• Gene Summary: 'Dermatopontin is an extracellular matrix protein with possible functions in cell-matrix interactions and matrix assembly. The protein is found in various tissues and many of its tyrosine residues are sulphated. Dermatopontin is postulated to modify the behavior of TGF-beta through interaction with decorin. [provided by RefSeq, Jul 2008]'