Amphiphysin (AMPH) (NM_001635) Human Untagged Clone

CAT#: SC119115

AMPH (untagged)-Human amphiphysin (AMPH), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AMPH
• Synonyms: AMPH1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001635, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCGACATCAAGACGGGCATCTTCGCCAAGAACGTCCAGAAGCGACTCAACCGCGCGCAGGAAAAGG
  TCCTCCAAAAGCTGGGGAAAGCTGATGAGACAAAAGACGAACAGTTCGAAGAATATGTCCAGAACTTCAA
  ACGGCAAGAAGCAGAGGGTACCAGACTTCAGCGAGAACTCCGAGGATATTTAGCAGCAATCAAAGGCATG
  CAGGAGGCCTCCATGAAGCTCACAGAGTCGCTGCATGAAGTCTATGAGCCTGACTGGTATGGGCGGGAAG
  ATGTGAAAATGGTTGGTGAGAAATGTGATGTGCTGTGGGAAGACTTCCATCAAAAACTCGTGGATGGGTC
  CTTGCTAACACTGGATACCTACCTGGGGCAATTTCCTGACATAAAGAATCGCATCGCCAAGCGCAGCAGG
  AAGCTAGTGGACTATGACAGTGCCCGCCACCATCTGGAAGCTCTGCAGAGCTCCAAGAGGAAGGATGAGA
  GTCGAATCTCTAAGGCAGAAGAAGAATTTCAGAAAGCACAGAAAGTGTTTGAAGAGTTTAACGTTGACTT
  ACAAGAAGAGTTACCATCATTATGGTCAAGACGAGTTGGATTTTATGTTAATACTTTCAAAAACGTCTCC
  AGCCTTGAAGCCAAGTTTCATAAGGAAATTGCGGTGCTTTGCCACAAACTGTATGAAGTGATGACAAAAC
  TGGGTGACCAGCACGCCGACAAGGCCTTCACCATCCAAGGAGCGCCCAGTGATTCGGGTCCTCTCCGCAT
  TGCAAAGACACCATCACCGCCTGAGGAGCCTTCACCCCTCCCGAGCCCGACAGCAAGTCCAAATCATACA
  TTAGCACCTGCGTCTCCCGCACCAGCACGGCCTCGGTCACCTTCACAGACAAGGAAAGGGCCTCCTGTCC
  CACCTCTACCTAAAGTCACCCCGACAAAGGAACTGCAGCAGGAGAACATCATCAGTTTCTTTGAGGACAA
  CTTTGTTCCAGAAATCAGTGTGACAACACCTTCCCAGAATGAAGTCCCTGAGGTGAAGAAAGAGGAGACT
  TTGCTGGATCTGGACTTTGATCCTTTCAAGCCCGAGGTGACACCTGCAGGTTCTGCTGGAGTGACCCACT
  CACCCATGTCTCAGACATTGCCCTGGGACCTATGGACGACAAGCACTGATTTGGTACAGCCGGCTTCTGG
  TGGTTCATTTAATGGATTCACACAGCCCCAGGATACTTCATTATTCACAATGCAGACAGACCAGAGTATG
  ATCTGCAACTTGGCTGAATCTGAACAGGCTCCACCCACAGAGCCAAAAGCAGAGGAGCCTCTGGCTGCTG
  TCACACCTGCCGTTGGTCTGGACCTTGGAATGGACACTCGGGCTGAGGAGCCAGTGGAGGAGGCAGTGAT
  CATACCTGGAGCTGATGCTGATGCAGCTGTTGGAACCTTGGTGTCAGCAGCTGAGGGGGCCCCAGGAGAG
  GAAGCAGAGGCGGAGAAGGCCACTGTCCCTGCCGGGGAAGGAGTAAGTTTAGAGGAGGCCAAAATTGGAA
  CTGAAACCACTGAGGGTGCAGAGAGTGCCCAACCTGAAGCAGAGGAGCTCGAAGCAACAGTGCCTCAGGA
  GAAGGTCATTCCTTCGGTGGTCATAGAGCCTGCCTCCAACCATGAAGAGGAAGGAGAAAACGAAATAACT
  ATAGGTGCAGAGCCCAAGGAGACCACCGAGGACGCGGCTCCTCCGGGCCCCACCAGCGAGACACCGGAGC
  TGGCTACGGAGCAGAAGCCTATCCAGGACCCTCAGCCCACGCCTTCTGCACCAGCCATGGGGGCTGCTGA
  CCAGCTAGCATCTGCAAGGGAGGCCTCTCAGGAATTGCCTCCTGGCTTTCTCTACAAGGTGGAAACACTG
  CATGATTTTGAGGCAGCAAATTCTGATGAACTTACCTTACAAAGGGGTGATGTGGTGCTGGTGGTCCCCT
  CAGATTCAGAAGCTGATCAGGATGCAGGCTGGCTGGTGGGAGTGAAGGAATCAGACTGGCTTCAGTACAG
  AGACCTTGCCACCTACAAAGGCCTCTTTCCAGAGAACTTCACCCGACGCTTAGATTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001635 unedited
  GCGATTTGTATACGACTACTATAGGCGGCCGCGCAATTCGCACGAGGGCCGCCAGCCCCG
  CTGCGCGCTCTGCTCTTCGCAGCTCCCCGGACCCGCAGCCATGGCCGACATCAAGACGGG
  CATCTTCGCCAAGAACGTCCAGAAGCGACTCAACCGCGCGCAGGAAAAGGTCCTCCAAAA
  GCTGGGGAAAGCTGATGAGACAAAAGACTAACAGTTCGAAGAATATGTTCAGAACTTCAA
  ACGGCAAGAAGCAGAGGGTACCAGACTTCAGCGAGAACTCCGAGGATATTTAGCAGCAAT
  CAAAGGCATGCAGGAGGCCTCCATGAAGCTCACAGAGTCGCTGCATGAAGTCTATGAGCC
  TGACTGGTATGGGCGGGAAGATGTGAAAATGGTTGGTGAGAAATGTGATGTGCTGTGGGA
  AGACTTCCATCAAAAACTCGTGGATGGGTCCTTGCTAACACTGGATACCTACCTGGGGCA
  ATTTCCTGACATAAAGAATCGCATCGCCAAGCGCATCAGGAAGCTAGTGGACTATGACAG
  TGCCCCGCCACCATCTGGAAGCTCTGCAGAGCTCCAAGAGGAAGGATGACAGTCGAATCT
  TTAATGCACAAGAACAATTTCACAAAGCACAAAAAGTGTTTGAAGAGTTTAACGTTGACT
  TACAATAAGAGTACCATCATTATGGTCCAGACGAGTTGGATTTTATGCTAATCCTTTCAC
  AAACGTCTCCAGCCCTTTGAGCCCAACTTCATAAAGAAAATTGCGGGGCTTTGTCCCAAA
  CTGTTTGAAATGAAGACCCAACTGGGTGACCACTACCCCGACAAGCCTTCACATTCCAAG
  AGCGCCCCAGGATTCGGGTCCTCTCCGCATCGCAAAAACACATCACCGCCTGAGGAGCCT
  TACACCCTCCGAACCCGCACGGGGTCCCAAATTACATTTCCACCTGGGCTCCCCGACCAA
  AACGGCTTCGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001635 unedited
  NNTTTTAGCTCTGGACCCGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTAGGGGAATCGGTGAAGATTACCAAAGGGTTATTTGG
  AATGACACAGCACTGAAAACATAATTGTTACAGATGATTTGGGGATACAGCATACACCAT
  CTATTTTACTTTAAAACAATCTGGGAAAATGAGTTGCATAAATAAAAAGAGGGGGAAATA
  TAGAGGAGCTGGTTTTATAGGGGTCTTTTGGGGGTAAATGAATATGCCCCATCTTTTTAC
  CCAATCTCATAAAGGCAGAAAAAAAAACTGGTTAACTGCCCATCCCAACTAGCCTACCTC
  CAGCCACAGGGGGTGGACAGCTAGATAAATCAGGCCCTGCCTTCTCTGAGGGGGCCTCAG
  GGGAAACGGGCTATCTGGCATCTCATCCTGAAAACTGCACAGTACAAACACAGATACTGG
  TGACTTTTTTGGCAATGGGATGGCGTGGTTTTGCCATTAGGCAAAACTGCAATTGTAATG
  CCAAACAGAATAAAAGCAATGCATGTATCAATGACAATTGAGAGGCAAAGAGCTACAATG
  GAAAGGAAAGAAGCAACTATGTATATGTAGCCTTCTGCATACACATAGATCCAGGAGTCA
  TGGGAACTAACTTGCGTGACTGTGTGTCCATAATATTTAGAAGGGGGTGGAAAAGAACAT
  TGAGTACTTTTGCAGCAGTTTTAGTTTAAACATAGTAAACCTGAAAGGGAGCGAGAGCAC
  GTGCTCCACCTGGGCAGGGGAATAGAAAATCCTGGGCTACAGATAATTATCACATTTTTG
  CAGGGCAGCCCTCATACGATCACCCGATGCGAAAATTAAGGCTGACCTGGAAAGCTCTTA
  AA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001635
• Insert Size: 3150 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001635.2, NP_001626.1
• RefSeq Size: 3287 bp
• RefSeq ORF: 2088 bp
• Locus ID: 273
• Cytogenetics: 7p14.1
• Domains: SH3, BAR, BAR
• Protein Families: Secreted Protein
• Protein Pathways: Fc gamma R-mediated phagocytosis
• Gene Summary: 'This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Nov 2010]'
  Transcript Variant: This variant (1) encodes the longer isoform (1).