Cytokeratin 14 (KRT14) (NM_000526) Human Untagged Clone

CAT#: SC119817

KRT14 (untagged)-Human keratin 14 (KRT14)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KRT14
• Synonyms: CK14; EBS3; EBS4; K14; NFJ
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119817 sequence for NM_000526 edited (data generated by NextGen Sequencing)
  ATGACTACCTGCAGCCGCCAGTTCACCTCCTCCAGCTCCATGAAGGGCTCCTGCGGCATC
  GGGGGCGGCATCGGGGGCGGCTCCAGCCGCATCTCCTCCGTCCTGGCCGGAGGGTCCTGC
  CGCGCCCCCAGCACCTACGGGGGCGGCCTGTCTGTCTCATCCTCCCGCTTCTCCTCTGGG
  GGAGCCTATGGGTTGGGGGGCGGCTATGGCGGTGGCTTCAGCAGCAGCAGCAGCAGCTTT
  GGTAGTGGCTTTGGGGGAGGATATGGTGGTGGCCTTGGTGCTGGCTTGGGTGGTGGCTTT
  GGTGGTGGCTTTGCTGGTGGTGATGGGCTTCTGGTGGGCAGTGAGAAGGTGACCATGCAG
  AACCTCAACGACCGCCTGGCCTCCTACCTGGACAAGGTGCGTGCTCTGGAGGAGGCCAAC
  GCCGACCTGGAAGTGAAGATCCGTGACTGGTACCAGAGGCAGCGGCCTGCTGAGATCAAA
  GACTACAGTCCCTACTTCAAGACCATTGAGGACCTGAGGAACAAGATTCTCACAGCCACA
  GTGGACAATGCCAATGTCCTTCTGCAGATTGACAATGCCCGTCTGGCCGCGGATGACTTC
  CGCACCAAGTATGAGACAGAGTTGAACCTGCGCATGAGTGTGGAAGCCGACATCAATGGC
  CTGCGCAGGGTGCTGGACGAACTGACCCTGGCCAGAGCTGACCTGGAGATGCAGATTGAG
  AGCCTGAAGGAGGAGCTGGCCTACCTGAAGAAGAACCACGAGGAGGAGATGAATGCCCTG
  AGAGGCCAGGTGGGTGGAGATGTCAATGTGGAGATGGACGCTGCACCTGGCGTGGACCTG
  AGCCGCATTCTGAACGAGATGCGTGACCAGTATGAGAAGATGGCAGAGAAGAACCGCAAG
  GATGCCGAGGAATGGTTCTTCACCAAGACAGAGGAGCTGAACCGCGAGGTGGCCACCAAC
  AGCGAGCTGGTGCAGAGCGGCAAGAGCGAGATCTCGGAGCTCCGGCGCACCATGCAGAAC
  CTGGAGATTGAGCTGCAGTCCCAGCTCAGCATGAAAGCATCCCTGGAGAACAGCCTGGAG
  GAGACCAAAGGTCGCTACTGCATGCAGCTGGCCCAGATCCAGGAGATGATTGGCAGCGTG
  GAGGAGCAGCTGGCCCAGCTCCGCTGCGAGATGGAGCAGCAGAACCAGGAGTACAAGATC
  CTGCTGGACGTGAAGACGCGGCTGGAGCAGGAGATCGCCACCTACCGCCGCCTGCTGGAG
  GGCGAGGACGCCCACCTCTCCTCCTCCCAGTTCTCCTCTGGATCGCAGTCATCCAGAGAT
  GTGACCTCCTCCAGCCGCCAAATCCGCACCAAGGTCATGGATGTGCACGATGGCAAGGTG
  GTGTCCACCCACGAGCAGGTCCTTCGCACCAAGAACTGA
  
  Clone variation with respect to NM_000526.4
  6 c=>t;189 c=>t;193 c=>t;369 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000526 unedited
  NNGGGTGCANAATATGTATACGACTCACTATAGGCGGCCCGCGAATTCGCACCAGCTTCT
  CTTCACTCAGCCAACTGCTCGCTCGCTCACCTCCCTCCTCTGCACCATGACTACCTGCAG
  CCGCCAGTTCACCTCCTCCAGCTCCATGAAGGGCTCCTGCGGCATCGGGGGCGGCATCGG
  GGGCGGCTCCAGCCGCATCTCCTCCGTCCTGGCCGGAGGGTCCTGCCGCGCCCCCAGCAC
  CTACGGGGGCGGCCTGTCTGTCTCATCCTCCCGCTTCTCCTCTGGGGGAGCCTATGGGTT
  GGGGGGCGGCTATGGCGGTGGCTTCAGCAGCAGCAGCAGCAGCTTTGGTAGTGGCTTTGG
  GGGAGGATATGGTGGTGGCCTTGGTGCTGGCTTGGGTGGTGGCTTTGGTGGTGGCTTTGC
  TGGTGGTGATGGGCTTCTGGTGGGCAGTGAGAAGGTGACCATGCAGAACCTCAACGACCG
  CCTGGCCTCCTACCTGGACAAGGTGCGTGCTCTGGAGGAGGCCAACGCCGACCTGGAAGT
  GAAGATCCGTGACTGGTACCAGAGGCAGCGGCCTGCTGAGATCAAAGACTACAGTCCCTA
  CTTCAAGACCATTGAGGACCTGAGGAACAAGATTCTCACAGCCACAGTGGACAATGCCAA
  TGTCCTTCTGCAGATTGACAATGCCCGTCTGGCCGCGGATGACTTCCGCACCAAGTATGA
  GACAGAGTTGAACCTGCGCATGAGTGTGGAAGCCGACATCAATGGCCTGCGCCAGGTGCT
  GTACGAACTGACCCTGGCCAGAGCTGACCTGGAGATGCAGATTGAGAGCCTGAGGAGGAG
  CTGGCCTACCTCGAGAAGACCACCGAGAGAGATGAATGCCCTGANAGCCCAGTGGGTGGA
  GATGTCATGTGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000526 unedited
  GGGCCTCCTGNACCCGGCCGCTTTCTANGATCGGTTTTTTTTTTTTTTTTTTATGCAACT
  CAGATAATGAAGCTGTATTGATTGCCAGGAGGGGGTGAGGGTGAAGCAGGGTCCAGCTGT
  GAAGTGCTTGGGCAGGAGAGGGGATCTTCCAGTGGGATCTGTGTCCACACGGGGGGCCTC
  CTAGGCCTGAGCGGGGCTGGGCAGCCTCAGTTCTTGGTGCGAAGGACCTGCTCGTGGGTG
  GACACCACCTTGCCATCGTGCACATCCATGACCTTGGTGCGGATTTGGCGGCTGGAGGAG
  GTCACATCTCTGGATGACTGCTATCCACAGGATAACTGTGAGGATGAGAGGTGGGCGTCC
  TCTCCCTCCACATGCGGCGGTCGGTGGCTATCTTCTGTCTCATCCTTGCCTTTCCTTCCA
  CCCGATCTTGTTCCTCCTGATTCGCTCCGCTCCCTCCTCCCTCGCCTTCTCGCCCCTCGC
  CTCTCCCTCCTCCTCCTCCCCACCTTCCCTCCCGCTCACACTCCCTGTCCTGCCCTCTTC
  GCTCCTTATTCCCATTATCCTTCTGCGTTCTTCCCCCTATCCATCTCCTTCACCACCTAC
  CCCTCCTCGCTCCTCATCCTCCCTCTCCCTTCTTCTTCTTTTTTTACCCCTCCTCTTCGT
  CCCCCTATCCCCTTGCCATTGGACTCTGTTCGCTTTTCCTCCTCTTCCCTCTCCATTCTC
  TTTTCCTCCTCTCTTCTCCTCCTTCTTTTCCTCCCCTATCTCCCCCTATTCCCGTCCTCT
  TCCCTCCTATCTCTTCACGTTCACATCTCCGTCCTACCTTTTTATTCACCCATCTCTTCT
  ACCCGCCACCATTCCTTTTCTTTCTTCCACTCCTTCTAGGGTTTGCGCTCTTCCACCCGC
  CTCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000526
• Insert Size: 1710 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000526.3, NP_000517.2
• RefSeq Size: 1634 bp
• RefSeq ORF: 1419 bp
• Locus ID: 3861
• Cytogenetics: 17q21.2
• Domains: filament
• Gene Summary: 'This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008]'