Glucose 6 phosphate isomerase (GPI) (NM_000175) Human Untagged Clone

CAT#: SC120063

GPI (untagged)-Human glucose-6-phosphate isomerase (GPI), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GPI
• Synonyms: AMF; GNPI; NLK; PGI; PHI; SA-36; SA36
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120063 sequence for NM_000175 edited (data generated by NextGen Sequencing)
  ATGGCCGCTCTCACCCGGGACCCCCAGTTCCAGAAGCTGCAGCAATGGTACCGCGAGCAC
  CGCTCCGAGCTGAACCTGCGCCGCCTCTTCGATGCCAACAAGGACCGCTTCAACCACTTC
  AGCTTGACCCTCAACACCAACCATGGGCATATCCTGGTGGATTACTCCAAGAACCTGGTG
  ACGGAGGACGTGATGCGGATGCTGGTGGACTTGGCCAAGTCCAGGGGCGTGGAGGCCGCC
  CGGGAGCGGATGTTCAATGGTGAGAAGATCAACTACACCGAGGGTCGAGCCGTGCTGCAC
  GTGGCTCTGCGGAACCGGTCAAACACACCCATCCTGGTAGACGGCAAGGATGTGATGCCA
  GAGGTCAACAAGGTTCTGGACAAGATGAAGTCTTTCTGCCAGCGTGTCCGGAGCGGTGAC
  TGGAAGGGGTACACAGGCAAGACCATCACGGACGTCATCAACATTGGCATTGGCGGCTCC
  GACCTGGGACCCCTCATGGTGACTGAAGCCCTTAAGCCATACTCTTCAGGAGGTCCCCGC
  GTCTGGTATGTCTCCAACATTGATGGAACTCACATTGCCAAAACCCTGGCCCAGCTGAAC
  CCCGAGTCCTCCCTGTTCATCATTGCCTCCAAGACCTTTACTACCCAGGAGACCATCACG
  AATGCAGAGACGGCGAAGGAGTGGTTTCTCCAGGCGGCCAAGGATCCTTCTGCAGTGGCG
  AAGCACTTTGTTGCCCTGTCTACTAACACAACCAAAGTGAAGGAGTTTGGAATTGACCCT
  CAAAACATGTTCGAGTTCTGGGATTGGGTGGGAGGACGCTACTCGCTGTGGTCGGCCATC
  GGACTCTCCATTGCCCTGCACGTGGGTTTTGACAACTTCGAGCAGCTGCTCTCGGGGGCT
  CACTGGATGGACCAGCACTTCCGCACGACGCCCCTGGAGAAGAACGCCCCCGTCTTGCTG
  GCCCTGCTGGGTATCTGGTACATCAACTGCTTTGGGTGTGAGACACACGCCATGCTGCCC
  TATGACCAGTACCTGCACCGCTTTGCTGCGTACTTCCAGCAGGGCGACATGGAGTCCAAT
  GGGAAATACATCACCAAATCTGGAACCCGTGTGGACCACCAGACAGGCCCCATTGTGTGG
  GGGGAGCCAGGGACCAATGGCCAGCATGCTTTTTACCAGCTCATCCACCAAGGCACCAAG
  ATGATACCCTGTGACTTCCTCATCCCGGTCCAGACCCAGCACCCCATACGGAAGGGTCTG
  CATCACAAGATCCTCCTGGCCAACTTCTTGGCCCAGACAGAGGCCCTGATGAGGGGAAAA
  TCGACGGAGGAGGCCCGAAAGGAGCTCCAGGCTGCGGGCAAGAGTCCAGAGGACCTTGAG
  AGGCTGCTGCCACATAAGGTCTTTGAAGGAAATCGCCCAACCAACTCTATTGTGTTCACC
  AAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCATGTATGAGCACAAGATCTTCGTT
  CAGGGCATCATCTGGGACATCAACAGCTTTGACCAGTGGGGAGTGGAGCTGGGAAAGCAG
  CTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCT
  TCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAATAA
  
  Clone variation with respect to NM_000175.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000175 unedited
  TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTAGTCCCGCCATGGC
  CGCTCTCACCCGGGACCCCCAGTTCCAGAAGCTGCAGCAATGGTACCGCGAGCACCGCTC
  CGAGCTGAACCTGCGCCGCCTCTTCGATGCCAACAAGGACCGCTTCAACCACTTCAGCTT
  GACCCTCAACACCAACCATGGGCATATCCTGGTGGATTACTCCAAGAACCTGGTGACGGA
  GGACGTGATGCGGATGCTGGTGGACTTGGCCAAGTCCAGGGGCGTGGAGGCCGCCCGGGA
  GCGGATGTTCAATGGTGAGAAGATCAACTACACCGAGGGTCGAGCCGTGCTGCACGTGGC
  TCTGCGGAACCGGTCAAACACACCCATCCTGGTAGACGGCAAGGATGTGATGCCAGAGGT
  CAACAAGGTTCTGGACAAGATGAAGTCTTTCTGCCAGCGTGTCCGGAGCGGTGACTGGAA
  GGGGTACACAGGCAAGACCATCACGGACGTCATCAACATTGGCATTGGCGGCTCCGACCT
  GNGACCCCTCATGGTGACTGAAGCCCTTAAGCCATACTCTTCAGGAGGTCCCCGCGTCTG
  GTATGTCTCCAACATTGATGGAACTCACATTGCCAAAACCCTGGCCCAGCTGAACCCCGA
  GTCCTCCCTGTTCATCATTGCCTNCAAGACCTTTACTACCCCAGAGACCATCACGAATGC
  AAAGACGGCGAAGGAGTGGTTTCTCCAGGCGGGCAAAGATCCTTCTGCAGTGGCGAAACA
  CTTTGTTGCCCTGTCTACTAACCCACCAAAGTGAAGAGTTTGGGATTGACCCTCAAACAT
  GTTCCAGTTCTGGGATTGGTGGGGAGACGCTACTCCTGTGTCCGGCATCGGACTTTCATT
  GCCCTGCACGGGGTTTTGAACACTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000175 unedited
  TTACCGCGGCCGCATCTANATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTGCAAAAGAACACCAGTTTATTGGACTCTGGCCTCGCGCTGCTGCTTGATGAAA
  TTGATGAACCCATTGGTAAAAACGTCGTGAGAGGGCACTTGAGCACTGCCATCAAGCTCA
  GGCTTTATTTTCTTAACCAGCTGGTTTCCCAGCTCCACTCCCCACTGGTCAAAGCTGGTG
  ATGTCCCAAATGATGCCCTGAACGAAAATCTTGGGCTCATACATGGGGACCAAGGGTCCA
  AACATGAATGGGGTGAGCTTGGGGAACACAATAAAGTTGGGTGGGCGATTTCCTTCAAAG
  ACCTTATGTGGCAGCAGCCTCTCAAGGGCCTCTGGACTCTTGCCCGCAACCTGGAGCTCC
  TTTCGGGCCTCCTCCGGCGATTTTCCCCTCATCAAGGCCTCTGTCTGGGCCAAAAAATTG
  GCCAGGAGGATCTTGTGATGCAAACCCTTCCCTATGGGGGGCTGGGTCTGGACCGGGATG
  AGGAAATCACAGGGTATCATCTTGGTGCCTTGGTGGATGAGCTGGTAAAAAACATGCTGG
  CCATTGGTCCCTGGCTCCCCCCCACAATGGGGCCTGTCTGGTGGGCCCACCGGTTCCAAA
  ATTGGTGATGTTTTTCCCATTGGACTCCATGGCGCCCTGGTGGAAGTACCCAACAAAACG
  GGGCAGGTCTGGTCATAGGGCACCATGGGGTGGGCTCACACCCAAGCAGTTGATGTCCAA
  ATCCCCACAGGGCCACAAGACGGGGGCCTTTTTTTCAGGGGCGTTTTGCGGAAATGCTGG
  TCCTCACATGGAGCCCCCCAGAACACTTGTTTAAAATTGTCAAAACCCCCTCCCGGGGCA
  TGGAAAAGCCCTAGGCCACCCCACGAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000175
• Insert Size: 1750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000175.2, NP_000166.2
• RefSeq Size: 2075 bp
• RefSeq ORF: 1677 bp
• Locus ID: 2821
• Cytogenetics: 19q13.11
• Domains: PGI
• Protein Families: Druggable Genome
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pentose phosphate pathway, Starch and sucrose metabolism
• Gene Summary: 'This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phosphate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]'
  Transcript Variant: This variant (2) differs in the 5' UTR and uses a downstream start codon, compared to variant 3. The encoded isoform (2) has a shorter N-terminus, compared to isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.