Troponin I fast skeletal muscle (TNNI2) (NM_003282) Human Untagged Clone

CAT#: SC124050

TNNI2 (untagged)-Human troponin I type 2 (skeletal, fast) (TNNI2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TNNI2
• Synonyms: AMCD2B; DA2B; DA2B1; FSSV; fsTnI
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124050 sequence for NM_003282 edited (data generated by NextGen Sequencing)
  ATGGGAGATGAGGAGAAGCGGAACAGGGCCATCACGGCCCGCAGGCAGCACCTGAAGAGC
  GTGATGCTGCAGATAGCGGCCACGGAGCTGGAGAAGGAGGAGAGCCGCCGTGAGGCAGAG
  AAGCAGAACTACCTGGCGGAGCACTGCCCGCCGCTGCATATCCCGGGCTCCATGTCTGAA
  GTGCAGGAGCTCTGCAAACAGCTGCACGCCAAGATCGATGCGGCTGAAGAGGAGAAGTAC
  GACATGGAGGTGAGGGTGCAGAAGACCAGCAAGGAGCTGGAGGACATGAACCAGAAGCTA
  TTTGATCTGCGGGGCAAGTTCAAGCGGCCCCCACTGCGGAGGGTGCGCATGTCGGCCGAT
  GCCATGCTCAAGGCCCTGCTGGGCTCGAAGCACAAGGTGTGCATGGACCTGAGGGCCAAC
  CTGAAGCAGGTCAAGAAGGAGGACACAGAGAAGGAGCGGGACCTGCGAGACGTGGGTGAC
  TGGAGGAAGAACATCGAGGAGAAGTCTGGCATGGAGGGCCGGAAGAAGATGTTTGAGTCC
  GAGTCCTAG
  
  Clone variation with respect to NM_003282.3
  60 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003282 unedited
  CACTATAGGGCGGCCGCGAATTTAAGAGATTGTCCTTGAAGGAGGCTCCAAGCTCAGGAC
  CTCAGGATGGGAGATGAGGAGAAGCGGAACAGGGCCATCACGGCCCGCAGGCAGCACCTG
  AAGAGCGTGATGCTGCAGATAGCGGCCACGGAGCTGGAGAAGGAGGAGAGCCGCCGTGAG
  GCAGAGAAGCAGAACTACCTGGCGGAGCACTGCCCGCCGCTGCATATCCCGGGCTCCATG
  TCTGAAGTGCAGGAGCTCTGCAAACAGCTGCACGCCAAGATCGATGCCGGCTGAAGAGGA
  GAAGTACGACATGGAGGTGAGGGTGCAGAAGACCAGCAAGGAGCTGGAGGACATGAACCA
  GAAGCTATTTGATCTGCGGGGCAAGTTCAAGCGGCCCCCACTGCGGAGGGTGCGCATGTC
  GGCCGATGCCATGCTCAAGGCCCTGCTGGGCTCGAAGCACAAGGTGTGCATGGACCTGAG
  GGCCAACCTGAAGCAGGTCAAGAAGGAGGACACAGAGAAGGAGCGGGACCTGCGAGACGT
  GNGTGACTGGAGGAAGAACATCGAGGAGAAGTCTGGCATGGAGGGCCGGAAGAAGATGTT
  TGAGTCCGAGTCCTAGGCCACTCGCTGCCCCTACCCCTGCCCCGGTGCCCGGCTCCCAGC
  AGAACATACTANGGAGATGCACCCAGAGCCCTGCCAGGAGGGCTGGCCTCACCACCACCG
  TCAATAAAGGATTTGAATCCCCAAAAAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003282 unedited
  NCCCCAGAGGCACTGGGGAGGGGTACAGGGATGCCACCCGGGATCTGTTCAGGAAACAGC
  TATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTGGTTTTTTTGGGGATTCAA
  ATCCTTTATTGACGGTGGTGGTGAGGCCAGCCCTCCCTGGCAGGCTCTGGGTGCATCTCC
  CTAGTATGTTCTGCTGGGAGCCGGGCACCGGGGCAGGCGTAGGGGCAGCGAGTGGCCTAT
  GACTCGGACTCAAACATCTTCTTTCCGGCCCTCCATGCCAGACTTCTCCTCGATGTTCTT
  CCTCCAGTCACCCACGTCTCGCAGGTCCCGCTCCTTCTCTGTGTCCTCCTTCTTGACCTG
  CTTCAGGTTGGCCCTCAGGTCCATGCACACCTTGTGCTTCGAGCCCAGCANGGCCTTGAG
  CATGGCATCGGCCGACATGCGCACCCTCCGCAGTGGGGGCCGCTTGAACTTGCCCCGCAG
  ATCAAATAGCTTCTGGTTCATGTCCTCCAGCTCCTTGCTGGTCTTCTGCACCCTCACCTC
  CATGTCGTACTTCTCCTCTTCAGCCGCATCGATCTTGGCGTGCAGCTGTTTGCAGAGCTC
  CTGCACTTCANACATGGGAGCCCGGATATGCAGCGGCNGGCAGTNGCTCGCCAGTAGTTC
  TGCTTTCTGCTCACGGCGGCTCTCTCTTCTCAGCTCGTGGCGTATCTGACATCACGCTCT
  ANGTGTGCTC
  
• Restriction Sites: Please inquire
• ACCN: NM_003282
• Insert Size: 800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003282.2, NP_003273.1
• RefSeq Size: 700 bp
• RefSeq ORF: 549 bp
• Locus ID: 7136
• Cytogenetics: 11p15.5
• Domains: Troponin
• Gene Summary: 'This gene encodes a fast-twitch skeletal muscle protein, a member of the troponin I gene family, and a component of the troponin complex including troponin T, troponin C and troponin I subunits. The troponin complex, along with tropomyosin, is responsible for the calcium-dependent regulation of striated muscle contraction. Mouse studies show that this component is also present in vascular smooth muscle and may play a role in regulation of smooth muscle function. In addition to muscle tissues, this protein is found in corneal epithelium, cartilage where it is an inhibitor of angiogenesis to inhibit tumor growth and metastasis, and mammary gland where it functions as a co-activator of estrogen receptor-related receptor alpha. This protein also suppresses tumor growth in human ovarian carcinoma. Mutations in this gene cause myopathy and distal arthrogryposis type 2B. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009]'
  Transcript Variant: This variant (1) and variant 2 encode the same protein (isoform 1).