TPSAB1 (NM_003294) Human Untagged Clone

CAT#: SC124210

TPSAB1 (untagged)-Human tryptase alpha/beta 1 (TPSAB1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TPSAB1
• Synonyms: TPS1; TPS2; TPSB1; TPSB2; Tryptase-2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124210 sequence for NM_003294 edited (data generated by NextGen Sequencing)
  ATGCTGAATCTGCTGCTGCTGGCGCTGCCCGTCCTGGCGAGCCGCGCCTACGCGGCCCCT
  GCCCCAGGCCAGGCCCTGCAGCGAGTGGGCATCGNNGGGGGTCAGGAGGCCCCCAGGAGC
  AAGTGGCCCTGGCAGGTGAGCCTGAGAGTCCACGGCCCATACTGGATGCACTTCTGCGGG
  GGCTCCCTCATCCACCCCCAGTGGGTGCTGACCGCAGCGCACTGCGTGGGACCGGACGTC
  AAGGATCTGGCCGCCCTCAGGGTGCAACTGCGGGAGCAGCACCTCTACTACCAGGACCAG
  CTGCTGCCGGTCAGCAGGATCATCGTGCACCCACAGTTCTACACCGCCCAGATCGGAGCG
  GACATCGCCCTGCTGGAGCTGGAGGAGCCGGTGAAGGTCTCCAGCCACGTCCACACGGTC
  ACCCTGCCCCCTGCCTCAGAGACCTTCCCCCCGGGGATGCCGTGCTGGGTCACTGGCTGG
  GGCGATGTGGACAATGATGAGCGCCTCCCACCGCCATTTCCTCTGAAGCAGGTGAAGGTC
  CCCATAATGGAAAACCACATTTGTGACGCAAAATACCACCTTGGCGCCTACACGGGAGAC
  GACGTCCGCATCGTCCGTGACGACATGCTGTGTGCCGGGAACACCCGGAGGGACTCATGC
  CAGGGCGACTCCGGAGGGCCCCTGGTGTGCAAGGTGAATGGCACCTGGCTGCAGGCGGGC
  GTGGTCAGCTGGGGCGAGGGCTGTGCCCAGCCCAACCGGCCTGGCATCTACACCCGTGTC
  ACCTACTACTTGGACTGGATCCACCACTATGTCCCCAAAAAGCCGTGA
  
  Clone variation with respect to NM_003294.3
  95 t=>n;96 c=>n;396 c=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003294 unedited
  TTGTAATACGTTTTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGATGCTTTATCTG
  CTGCTGCTGGCGCTGCCCGTCCTGGCGAGCCGCGCCTACGCGGCCCCTGCCCCAGGCCAG
  GCCCTGCAGCGAGTGGGCATCGTTGGGGGTCAGGAGGCCCCCAGGAGCAAGTGGCCCTGG
  CAGGTGAGCCTGAGAGTCCACGGCCCATACTGGATGCACTTCTGCGGGGGCTCCCTCATC
  CACCCCCAGTGGGTGCTGACCGCAGCGCACTGCGTGGGACCGGACGTCAAGGATCTGGCC
  GCCCTCAGGGTGCAACTGCGGGAGCAGCACCTCTACTACCAGGACCAGCTGCTGCCGGTC
  AGCAGGATCATCGTGCACCCACAGTTCTACACCGCCCAGATCGGAGCGGACATCGCCCTG
  CTGGAGCTGGAGGAGCCGGTGAAGGTCTCCAGCCACGTCCACACGGTCACCCTGCCCCCT
  GCCTCAGAGACCTTCCCCCCGGGGATGCCGTGCTGGGTCACTGGCTGGGGCGATGTGGAC
  AATGATGAGCGCCCTCCACCGCCATTTCCTCTGAAGCAGGTGAAGGTCCCCATAATGGAA
  AACCACATTTGTGACGCAAAATACCACCTTGGCGCCTACACGGGAGACGACGTCCGCATC
  GTCCGTGACGACATGCTGTGTGCCCGGAACACCCCGAGGAACTCATGCCACGGCGACTCC
  GGACGCCCCCTGNTGTGCAAGGTGAATGGCACCTTGCTGCCAGCGGCCGTGTCAGCTGGG
  CGAGGGCTGTGCCACGCCAACCGGCCTGGCATTTCACCCGTGTCACCTACTACTTGGACT
  GGATCCACCCTATGTCCCCAAAAAGCCGGGAGTCACGCTCTGGTTGGCCACCTGGGTCCT
  GTAGGAACAACCCCTGTTGTACAAAACCCACTGCTTCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003294 unedited
  ACTATGAACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGATTTCCAT
  GCACTTTAATGAGGTCCAGCACTCAGGAGGATTAGCGCCCACCACCAGCTGCCTGGGCAG
  GGGAGGGCCGGAGGGCCCAGTGCAGGCGTCAGGCTTAGGACAGGGAAGGGGGCTCAGGAT
  GGGGAAGGGTCCTCAGGACAGGGGAAGGGGCTCAGAAGAGAGCAGGGGGCTTAGGACAGG
  AAGGGGCACTCAGGACGGGGCAGGGAAGGTGTGGGGGGCAGTCGCCACCTGGGTAGGAAG
  CAGTGGTGTTTTGGACAGCAGGGGTTGGTCCTCCAGTGACCCAGGTGGCCAACCCAGGCC
  TGACTCACGGCTTTTTGGGGACATAGTGGTGGATCCAGTCCAAGTAGTAGGTGACACGGG
  TGTAGATGCCAGGCCGGTTGGGCTGGGCACAGCCCTCGCCCCAGCTGACCACGCCCGCCT
  GCAGCCAGGTGCCATTCACCTTGCACACCAGGGGCCCTCCGGAGTCGCCCTGGCATGAGT
  CCCTCCGGGTGTTCCCGGCACACAGCATGTCGTCACGGACGATGCGGACGTCGTCTCCCG
  TGTAGGCGCCAAGGTGGTATTTTGCGTCACAAATGTGGTTTTCCATTATGGGGACCTTCA
  CCTGCTTCAGAGGAAATGGCGGTGGGAGGCGCTCATCATTGTCCACATCGCCCCAGCCAG
  TGACCCAGCACGGCATCCCCGGGGGGAAGGTCTCTGNAGCANGGGGCAGGGTGACCGTGT
  GGACCTGGCTGGAGACCTTTACGGNTNCTCCAGCTCAGCAGGCGATGTCCGCTCGATCTG
  GCGTGTAGACTTGGGTGCACGAGATACTGCTGACCGCAGCANCTGTCCTGGTATAGAGTG
  CTGCTCCCCANTTCACCCTGAGGGCGCCAGTACTTGACTCCGGTCCCGCATGCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003294
• Insert Size: 1300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003294.3, NP_003285.2
• RefSeq Size: 1194 bp
• RefSeq ORF: 828 bp
• Locus ID: 7177
• Cytogenetics: 16p13.3
• Domains: Tryp_SPc
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Gene Summary: 'Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq, Jul 2008]'