XRCC4 (BC005259) Human Untagged Clone

CAT#: SC126431

XRCC4 (untagged)-Human X-ray repair complementing defective repair in Chinese hamster cells 4, mRNA (cDNA clone MGC:12297 IMAGE:3838919), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: XRCC4
• Synonyms: DNA repair protein XRCC4; X-ray repair, complementing defective, repair in Chinese hamster; X-ray repair complementing defective repair in Chinese hamster cells 4; X-ray repair cross complementing protein 4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC005259 edited
  AAACCTTGATCTGTGAAAGCGGGCGTTTTGGAAGATACCGGAAGTAGAGTCACGGAGAGG
  TATTAAGAAATGGAGAGAAAAATAAGCAGAATCCACCTTGTTTCTGAACCCAGTATAACT
  CATTTTCTACAAGTATCTTGGGAGAAAACACTGGAATCTGGTTTTGTTATTACACTTACT
  GATGGTCATTCAGCATGGACTGGGACAGTTTCTGAATCAGAGATTTCCCAAGAAGCTGAT
  GACATGGCAATGGAAAAAGGGAAATATGTTGGTGAACTGAGAAAAGCATTGTTGTCAGGA
  GCAGGACCAGCTGATGTATACACGTTTAATTTTTCTAAAGAGTCTTGTTATTTCTTCTTT
  GAGAAAAACCTGAAAGATGTCTCATTCAGACTTGGTTCCTTCAACCTAGAGAAAGTTGAA
  AACCCAGCTGAAGTCATTAGAGAACTTATTTGTTATTGCTTGGACACCATTGCAGAAAAT
  CAAGCCAAAAATGAGCACCTGCAGAAAGAAAATGAAAGGCTTCTGAGAGATTGGAATGAT
  GTTCAAGGACGATTTGAAAAATGTGTGAGTGCTAAGGAAGCTTTGGAGACTGATCTTTAT
  AAGCGGTTTATTCTGGTGTTGAATGAGAAGAAAACAAAAATCAGAAGTTTGCATAATAAA
  TTATTAAATGCAGCTCAAGAACGAGAAAAGGACATCAAACAAGAAGGGGAAACTGCAATC
  TGTTCTGAAATGACTGCTGACCGAGATCCAGTCTATGATGAGAGTACTGATGAGGAAAGT
  GAAAACCAAACTGATCTCTCTGGGTTGGCTTCAGCTGCTGTAAGTAAAGATGATTCCATT
  ATTTCAAGTCTTGATGTCACTGATATTGCACCAAGTAGAAAAAGGAGACAGCGAATGCAA
  AGAAATCTTGGGACAGAACCTAAAATGGCTCCTCAGGAGAATCAGCTTCAAGAAAAGGAA
  AAAGGGAGGAAGAAGGAGACTTCAGAGAAAGAGGCCGTGTGATGGAAGCAAAATCAGAGA
  GAAAAGATGCTAAGCTGCTGCCTTTGAATAAGGAGGAACGTGAGCCAACAGATCCAAGAA
  CTGAAGCTCTAGGCAGTGGAAAAGCCAAGGAAACTGATCTTCCCTTCGAGCCTCCGGAGG
  GAGTGTAGCCCTGCCAACACCTTTATTTTAGCCCCATAACAGTCATTTCAGACTTGTGGC
  CTCCAGAATTTTAAGAGAATAAATTTTTATTGTATTAAGCCATGAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC005259 unedited
  TAGGATTTTGTAATACGACTCACTATAGGCGGCGCGCGAATTCGCCATTACGGCCGGGGA
  AACCTTGATCTGTGAAAGCGGGGGTTTTGGAGGATACCGGAAGTAGAGTCACGGAGAGGT
  ATTAAGAAATGGAGAGAAAAATAAGCAGAATCCACCTTGTTTCTGAACCCAGTATAACTC
  ATTTTCTACAAGTATCTTGGGAGAAAACACTGGAATCTGGTTTTGTTATTACACTTACTG
  ATGGGGATTCANCATGGACTGGGACAGTTTCTGAATCAGAGATTTCCCAAGAAGCTGATG
  ACATGGCAATGGAAAAAGGGAAATATGTTGGTGAACTGAGAAAAGCATTGTTGTCAGGAG
  CAGGACCAGCTGATGTATACACGTTTAATTTTTCTAAAGAGTCTTGTTATTTCTTCTTTG
  AGAAAAACCTGAAAGATGTCTCATTCAGACTTGGTTCCTTCAACCTAGAGAAAGTTGAAA
  ACCCAGCTGAAGTCATTAGAGAACTTATTTGTTATTGCTTGGACCCATTGGCAGAAATCA
  AGCCAAAATGAGCACCTGCCAAAAGAAATGAAAGGCTTCTGAGAGATGGGATGATGTTCA
  AGGACGATTTGAAAAATGTGTGAGTGCTAAGGAAGCTTTGGAGACTGATCTTTATAAGCG
  GGTTATTCTGGGGGTGGATGAGAAGGAAACAAAAATCAGAGATGTGGTAATAAATTATTA
  AATGCGGCTCAGGAACGAGAAAGGGCCTCAAACAGGAGGGGAAACTGCCATCTGGTCCTG
  AATGACTGCTGGCCCAGATCCACTCCATTATGAGGGTCCTGTTAGAGAAATGGAAACCCA
  CCCGGTCCCTCTGGGGTGGGTTCCCCTGGGGAAACGAAAAAGAGTCCCTTTTTTCAAGCC
  TTGGGGCCCTTGATTTGGCCCACTGAAAAAGGGGCCCGCAATGCGCAAAACCTTGGGGCA
  AACCC
  
• Restriction Sites: Please inquire
• ACCN: BC005259
• Insert Size: 1300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC005259.1, AAH05259.1
• RefSeq Size: 1274 bp
• Locus ID: 7518
• Cytogenetics: 5q14.2
• Protein Families: Druggable Genome
• Protein Pathways: Non-homologous end-joining
• Gene Summary: 'The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand breaks. This protein plays a role in both non-homologous end joining and the completion of V(D)J recombination. Mutations in this gene can cause short stature, microcephaly, and endocrine dysfunction (SSMED). Alternate transcript variants such as NM_022406 are unlikely to be expressed in some individuals due to a polymorphism (rs1805377) in the last splice acceptor site. [provided by RefSeq, Oct 2019]'