Ankyrin brain (ANK2) (NM_020977) Human Untagged Clone

CAT#: SC127419

ANK2 (untagged)-Human ankyrin 2, neuronal (ANK2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ANK2
• Synonyms: ANK-2; brank-2; LQT4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_020977, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATGAACGAAGATGCAGCTCAGAAAAGCGACAGTGGAGAGAAGTTCAACGGCAGTAGTCAGAGGAGAA
  AAAGACCCAAGAAGTCTGACAGCAATGCAAGCTTCCTCCGTGCTGCCAGAGCAGGCAACCTGGACAAAGT
  TGTGGAATATCTGAAGGGGGGCATAGACATCAATACCTGCAATCAGAATGGACTCAACGCTCTCCATCTG
  GCTGCCAAGGAAGGCCACGTGGGGCTGGTGCAGGAGCTGCTGGGAAGAGGGTCCTCTGTGGATTCTGCCA
  CTAAGAAGGGAAATACCGCTCTTCACATTGCATCTTTGGCTGGACAAGCAGAAGTTGTCAAAGTTCTTGT
  TAAGGAAGGAGCCAATATTAATGCACAGTCTCAGAATGGCTTTACTCCTTTATACATGGCTGCCCAAGAG
  AATCACATTGATGTTGTAAAATATTTGCTGGAAAATGGAGCTAATCAGAGCACTGCTACAGAGGATGGCT
  TTACTCCTCTAGCTGTGGCACTCCAGCAAGGACACAACCAGGCGGTGGCCATCCTCTTGGAGAATGACAC
  CAAAGGGAAAGTGAGGCTGCCAGCTCTGCATATTGCCGCTAGGAAAGACGACACCAAATCTGCCGCACTT
  CTGCTTCAGAATGACCACAATGCTGACGTACAATCCAAGATGATGGTGAATAGGACAACTGAGAGTGGTT
  TTACCCCTTTGCACATAGCTGCACATTACGGAAATGTCAACGTGGCAACTCTTCTTCTAAACCGGGGAGC
  TGCTGTGGACTTCACAGCCAGGAATGGAATCACTCCTCTGCATGTGGCTTCCAAAAGAGGAAATACAAAC
  ATGGTGAAGCTCTTACTGGATCGAGGCGGTCAGATCGATGCCAAAACTAGGGATGGGTTGACACCACTTC
  ACTGTGCTGCACGAAGTGGGCATGACCAAGTGGTGGAACTTCTGTTGGAACGGGGTGCCCCCTTGCTGGC
  AAGGACTAAGAATGGGCTGTCTCCACTACACATGGCTGCCCAGGGAGACCACGTGGAATGTGTGAAGCAC
  CTGTTACAGCACAAGGCACCTGTTGATGATGTCACCCTAGACTACCTGACAGCCCTCCACGTTGCTGCGC
  ACTGTGGCCACTACCGTGTAACCAAACTCCTTTTAGACAAGAGAGCCAATCCGAACGCCAGAGCCCTGAA
  TGGTTTTACTCCACTGCACATTGCCTGCAAGAAAAACCGCATCAAAGTCATGGAACTGCTGGTGAAATAT
  GGGGCTTCAATCCAAGCTATAACAGAGTCTGGCCTCACACCAATACATGTGGCTGCCTTCATGGGCCACT
  TGAACATTGTCCTCCTTCTGCTGCAGAACGGAGCCTCTCCAGATGTCACTAACATTCGTGGTGAGACGGC
  ACTACACATGGCAGCCCGAGCCGGGCAGGTGGAAGTGGTCCGATGCCTCCTGAGAAATGGTGCCCTTGTT
  GATGCCAGAGCCAGGGAGGAACAGACACCTTTACATATTGCCTCCCGCCTGGGTAAGACAGAAATTGTCC
  AGCTGCTTCTACAACATATGGCTCATCCAGATGCGGCCACTACAAATGGGTACACACCACTGCACATCTC
  TGCCCGGGAGGGCCAGGTGGATGTGGCATCAGTCCTATTGGAAGCAGGAGCAGCCCACTCCTTAGCTACC
  AAGAAGGGTTTTACTCCCCTGCATGTAGCAGCCAAGTATGGAAGCCTGGATGTGGCAAAACTTCTCTTGC
  AACGCCGTGCTGCCGCAGATTCTGCAGGGAAGAACGGCCTTACCCCGCTCCATGTTGCTGCTCATTATGA
  CAACCAGAAGGTGGCGCTGCTGTTACTGGAGAAGGGTGCTTCCCCTCATGCCACTGCCAAGAATGGCTAT
  ACTCCGTTACATATTGCTGCCAAGAAGAATCAAATGCAGATAGCTTCCACACTCCTGAACTATGGAGCAG
  AGACAAACATTGTGACAAAGCAAGGAGTAACTCCACTCCATCTGGCCTCGCAGGAGGGGCACACAGATAT
  GGTTACCTTGCTTCTGGATAAGGGAGCCAATATCCACATGTCAACTAAGAGTGGACTCACATCCTTACAC
  CTTGCAGCCCAGGAAGATAAAGTGAATGTTGCTGATATTCTCACCAAGCATGGAGCTGATCAGGATGCTC
  ATACAAAGCTTGGTTACACACCTTTAATTGTGGCCTGTCACTATGGAAATGTGAAAATGGTCAACTTTCT
  TCTGAAGCAGGGAGCAAATGTTAACGCAAAAACCAAGAACGGCTACACGCCTTTGCACCAGGCCGCTCAG
  CAGGGTCACACGCACATCATCAACGTCCTGCTCCAGCATGGGGCCAAGCCCAACGCCACCACTGCGAATG
  GCAACACTGCCTTGGCGATTGCTAAGCGTCTGGGCTACATCTCCGTGGTCGACACCCTGAAGGTTGTGAC
  TGAGGAGGTCACCACCACCACCACAACTATTACAGAAAAACACAAACTAAATGTACCTGAGACGATGACT
  GAGGTTCTTGATGTTTCTGATGAAGAGGGTGATGACACAATGACTGGTGATGGGGGAGAATACCTTAGGC
  CTGAGGACCTAAAAGAACTGGGTGATGACTCACTACCCAGCAGTCAGTTCCTGGATGGTATGAATTACCT
  GCGATACAGCTTGGAGGGAGGACGATCTGACAGCCTTCGATCCTTCAGTTCCGACAGGTCTCACACTCTG
  AGCCATGCCTCCTACCTGAGGGACAGTGCCGTGATGGATGACTCAGTTGTGATTCCCAGTCACCAGGTGT
  CAACTCTAGCCAAGGAGGCAGAAAGGAATTCTTATCGCCTAAGCTGGGGCACTGAGAACTTAGACAACGT
  GGCTCTTTCTTCTAGTCCTATTCATTCAGGTTTCCTGGTTAGTTTTATGGTGGATGCCCGAGGTGGTGCT
  ATGCGAGGATGCAGACACAATGGGCTCCGAATCATTATTCCACCTCGGAAATGTACTGCTCCAACGCGAG
  TCACCTGCCGACTGGTCAAGCGCCACAGACTGGCAACAATGCCTCCAATGGTGGAAGGAGAAGGCCTGGC
  CAGTCGCCTGATCGAAGTTGGACCTTCTGGTGCTCAGTTCCTTGGTAAACTTCACCTGCCAACGGCTCCT
  CCCCCACTTAATGAGGGAGAAAGTTTGGTCAGCCGCATTCTTCAGCTGGGGCCTCCTGGAACCAAATTCC
  TTGGGCCTGTGATCGTGGAGATCCCTCACTTTGCGGCCCTTCGAGGAAAGGAAAGGGAACTGGTGGTCCT
  GCGCAGTGAGAATGGGGACAGCTGGAAAGAGCATTTCTGTGACTACACTGAAGATGAATTGAATGAAATT
  CTTAACGGCATGGATGAAGTACTGGATAGCCCAGAAGACCTAGAAAAGAAACGAATCTGCCGCATCATCA
  CCCGAGACTTCCCACAGTACTTTGCAGTGGTGTCTCGTATCAAACAGGACAGCAATCTGATTGGCCCAGA
  AGGAGGTGTACTGAGCAGCACAGTGGTGCCCCAGGTGCAGGCCGTCTTCCCAGAGGGGGCACTCACCAAG
  CGGATCCGCGTAGGCCTGCAGGCTCAACCTATGCACAGTGAGCTGGTTAAGAAGATCCTAGGCAACAAAG
  CTACCTTCAGCCCTATAGTCACTTTGGAACCTAGAAGAAGAAAATTCCACAAACCAATTACCATGACCAT
  TCCTGTCCCCAAAGCTTCAAGTGATGTCATGTTGAATGGTTTTGGGGGAGATGCACCAACCTTAAGATTA
  CTATGCAGCATAACAGGTGGAACCACCCCTGCCCAGTGGGAAGATATTACAGGAACTACGCCATTAACAT
  TTGTCAATGAATGTGTTTCCTTTACAACAAACGTGTCTGCCAGGTTCTGGCTGATAGATTGTCGACAGAT
  CCAGGAATCCGTTACTTTTGCATCACAAGTATACAGAGAAATTATCTGCGTACCTTATATGGCCAAATTT
  GTAGTGTTTGCCAAATCACATGACCCCATTGAAGCCAGGTTGAGGTGTTTCTGCATGACTGATGATAAAG
  TGGATAAGACCCTTGAACAACAAGAAAATTTTGCTGAGGTGGCCAGAAGCAGGGATGTGGAGGTGTTAGA
  AGGAAAACCCATCTACGTTGATTGTTTCGGCAACTTGGTACCATTAACTAAAAGTGGCCAGCATCATATA
  TTCAGTTTTTTTGCCTTCAAAGAAAATAGACTTCCTCTATTTGTCAAGGTACGCGATACGACTCAGGAAC
  CTTGCGGACGACTATCATTTATGAAGGAGCCAAAATCCACGAGAGGCCTGGTGCATCAAGCTATTTGCAA
  CTTAAACATCACTTTGCCGATTTATACAAAGGAATCAGAGTCAGATCAAGAACAGGAGGAAGAGATCGAT
  ATGACATCAGAAAAAAATCCACAGGATGAGCAGGAACGGATCGAGGAAAGGCTGGCTTATATTGCTGATC
  ACCTTGGCTTCAGCTGGACAGAATTAGCAAGAGAACTGGATTTCACTGAGGAGCAAATTCATCAAATTCG
  AATTGAAAATCCCAACTCTCTTCAAGACCAGAGTCATGCACTGTTGAAGTACTGGCTAGAGAGGGATGGG
  AAACATGCTACAGATACCAACCTCGTTGAATGTCTCACCAAGATCAACCGAATGGATATTGTTCATCTCA
  TGGAGACCAACACAGAACCTCTCCAGGAGCGCATCAGTCATAGTTATGCAGAAATTGAACAGACCATTAC
  ACTGGATCATAGTGAAGGGTTCTCGGTACTTCAAGAGGAGTTATGCACTGCACAGCACAAGCAGAAAGAG
  GAGCAAGCTGTTTCTAAAGAAAGTGAGACCTGCGATCACCCTCCTATCGTCTCAGAGGAAGACATTTCTG
  TTGGTTATTCCACTTTTCAGGATGGCGTCCCCAAAACTGAGGGGGACAGCTCAGCAACAGCACTCTTTCC
  CCAAACTCACAAGGAGCAAGTTCAACAGGATTTCTCAGGGAAAATGCAAGACCTGCCTGAAGAGTCATCT
  CTGGAATATCAGCAGGAATATTTTGTGACAACTCCAGGAACAGAAACATCAGAGACTCAGAAGGCTATGA
  TAGTACCCAGCTCTCCCAGCAAGACACCTGAGGAAGTTAGCACCCCTGCAGAGGAGGAGAAGCTGTACCT
  CCAGACCCCAACATCCAGCGAGCGGGGAGGCTCTCCCATCATACAAGAACCCGAAGAGCCCTCAGAGCAC
  AGAGAGGAGAGCTCTCCGCGGAAAACCAGCCTCGTAATAGTGGAGTCTGCCGATAACCAGCCTGAGACCT
  GTGAAAGACTCGATGAAGATGCAGCTTTTGAAAAGGGAGACGATATGCCTGAAATACCCCCAGAAACAGT
  CACAGAAGAAGAATACATTGATGAGCATGGACACACCGTGGTAAAGAAGGTTACTAGGAAAATCATTAGG
  CGGTATGTATCCTCTGAAGGCACAGAGAAAGAAGAGATTATGGTGCAGGGAATGCCACAGGAACCTGTCA
  ACATCGAGGAAGGGGATGGCTATTCCAAAGTTATAAAGCGTGTTGTATTGAAGAGTGACACCGAGCAGTC
  AGAGGACAACAATGAGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020977 unedited
  AGCTTTTGTATACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGGTTTGATGGCAG
  AGATATTTTCTTTCCAAACTGTTCAAAATGATGAACGAAGATGCAGCTCAGAAAAGCGAC
  AGTGGAGAGAAGTTCAACGGCAGTAGTCAGAGGAGAAAAAGACCCAAGAAGTCTGACAGC
  AATGCAAGCTTCCTCCGTGCTGCCAGAGCAGGCAACCTGGACAAAGTTGTGGAATATCTG
  AAGGGGGGCATAGACATCAATACCTGCAATCAGAATGGACTCAACGCTCTCCATCTGGCT
  GCCAAGGAAGGCCACGTGGGGCTGGTGCAGGAGCTGCTGGGAAGAGGGTCCTCTGTGGAT
  TCTGCCACTAAGAAGGGAAATACCGCTCTTCACATTGCATCTTTGGCTGGACAAGCAGAA
  GTTGTCAAAGTTCTTGTTAAGGAAGGAGCCAATATTAATGCACAGTCTCAGAATGGCTTT
  ACTCCTTTATACATGGCTGCCCAAGAGAATCACATTGATGTTGTAAAATATTTGCTGGAA
  AATGGAGCTAATCAGAGCACTGCTACAGAGGATGGCTTTACTCCTCTAGCTGTGGCACTC
  CAGCAAGGACACAACCAGGCGGTGGCCATCCTCTTGGAGAATGACACCAAAGGGAAAGTG
  AGGCTGCCAGCTCTGCATATTGCCGCTAGGAAAGACGACACCAAATCTGCCGCACTTCTG
  CTTCAGAATGACCACAATGCTGACGTACAATCCAAGAGTGGTTTTACCCCTTTGCACATA
  GCTGCACATTACGGGAATGTCAACGTGGCAACTCTTCTTCTTAACCGGGAGCTGCTGTGG
  ACTCACAGCCAGGATGGGATCACTCCTCTGCATGTGGCTTCAAAGAAGAATACAACATGT
  GAGCTCNACTGGATCGAGCGTCAGACGTGCCAACTAGGNATGGTGACCCATT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020977 unedited
  GCCGCCTACAGGCNAAAGCCGTCTTTTTTCCCCCCCCCCCGGGTACGGNCTNAAAAACCC
  AATTTTACGGCCTTGNACACATCGCAGCTATGTATTGTTAGTGTTGTTTTTTTTTTTCCA
  TTAACTAATACATGCCCTCATAGATATATTCAATTAGTGTTATCACCATGGGAACAAGAT
  GCTGATTCGTCAACTGGAAAATTCACTTCTTCTCACAGTATTCAAGTTTTTGATAACACA
  GCAAAAATCAAAATCAAAAATTGTTTAATAACCATTATGTTGTTACACAGACATCATCTG
  CACTGCAAATAACACAACAGAAATGCTTTTCTTGAGCTCCAGTGAAGGTTTTGACAAGAA
  GGCCATAGGGTGTCTCAAAAAGTTTCACCATCGGCCTCTCTTATTTTGGCTGTCAAAGAA
  TGTCTTATTTCCTATCCAACTGCCAAAAAGTGCTAGCATTTGAGGCTTAGGAGATTTGGC
  TTGTGGGAAACCTCCCTCCCAGGAAATTTTTTCTCTTGGCAACATTACCTTAAATGATGA
  AGTGCCATTGCTTGCCGCATTTGTTTTTGACATTCGGAGCAATGAGTTATTTCTGCCCAC
  CAGGAAAAGACCACATAATTGGAAAATGCTCACTCCCTGGAGGATACACCCTGCCCTTTT
  TTATTTAAATTTCATCTTCATTGAATATACTTGTCTTTACCAACAGACTGTCCCCCCACG
  TATACCGTTTATCCCAATCTATTTGGGCCTATTTCCTTCCGTAAAAAGCTTCCCAGCCTT
  CCCCACCTTTAGTATCAGCGCGAGGCCTCGGCAATTCTTCAAATTCGTTCAGCCCCCTCC
  CTCTGCAACCCCCAATCCCTATCGCCCATATGCCCTTATAAATCCCCACGGCGGTTGCGC
  CCTTCAATTATTTAACCCCAATATNTTACCAAACCCTAACTCTCCTACGGTTTCCCCCCC
  CCCATCGTTCCCTCACAACCAATCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_020977
• Insert Size: 7680 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020977.2, NP_066187.2
• RefSeq Size: 7911 bp
• RefSeq ORF: 5619 bp
• Locus ID: 287
• Cytogenetics: 4q25-q26
• Domains: DEATH, ZU5, ANK
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a member of the ankyrin family of proteins that link the integral membrane proteins to the underlying spectrin-actin cytoskeleton. Ankyrins play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. The protein encoded by this gene is required for targeting and stability of Na/Ca exchanger 1 in cardiomyocytes. Mutations in this gene cause long QT syndrome 4 and cardiac arrhythmia syndrome. Multiple transcript variants encoding different isoforms have been described. [provided by RefSeq, Dec 2011]'
  Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. It encodes isoform 2 which is shorter than isoform 1. Isoform 2 is the major form of ankyrin in adult brain.