SH3BP2 (NM_003023) Human Untagged Clone

CAT#: SC128156

SH3BP2 (untagged)-Human SH3-domain binding protein 2 (SH3BP2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SH3BP2
• Synonyms: 3BP-2; 3BP2; CRBM; CRPM; RES4-23
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC128156 sequence for NM_003023 edited (data generated by NextGen Sequencing)
  ATGGCGGCTGAAGAGATGCATTGGCCTGTCCCTATGAAGGCCATTGGTGCCCAGAACCTG
  CTAACCATGCCTGGGGGCGTGGCCAAGGCTGGCTACCTGCACAAGAAGGGCGGTACCCAG
  CTGCAGCTGCTGAAATGGCCCCTGCGCTTTGTCATCATCCACAAACGCTGCGTCTACTAC
  TTCAAGAGTAGCACCTCTGCCTCCCCGCAGGGCGCCTTCTCCCTGAGTGGCTATAACCGG
  GTGATGCGGGCGGCTGAGGAGACCACGTCCAACAACGTTTTCCCCTTCAAGATCATCCAC
  ATCAGCAAGAAGCACCGCACGTGGTTCTTCTCGGCCTCCTCCGAGGAGGAGCGCAAGAGC
  TGGATGGCCTTGCTGCGCAGGGAGATTGGCCACTTCCACGAAAAGAAAGACCTGCCCTTG
  GACACCAGCGACTCCAGCTCGGACACAGACAGCTTCTACGGCGCAGTTGAGCGGCCTGTG
  GATATCAGCCTTTCCCCGTACCCCACGGACAATGAAGACTATGAGCACGACGATGAGGAT
  GACTCCTACCTGGAGCCTGACTCCCCGGAGCCCGGAAGGCTTGAGGATGCCCTGATGCAC
  CCACCGGCTTACCCACCACCCCCAGTGCCCACGCCCAGGAAGCCAGCCTTCTCTGACATG
  CCCCGGGCCCACTCCTTTACCTCCAAGGGCCCCGGTCCCCTACTGCCACCCCCGCCCCCT
  AAGCACGGCCTCCCAGATGTTGGCCTGGCTGCTGAGGACTCCAAGAGGGACCCACTGTGC
  CCGAGGCGGGCTGAGCCTTGCCCCAGGGTACCTGCTACCCCCCGAAGGATGAGCGATCCC
  CCTCTGAGCACCATGCCCACCGCACCCGGCCTCCGGAAACCCCCTTGCTTCCGGGAGAGT
  GCCAGCCCCAGCCCGGAGCCCTGGACCCCTGGCCACGGGGCCTGCTCCACTTCCAGTGCT
  GCCATCATGGCCACTGCCACCTCCAGAAACTGTGACAAACTCAAGTCCTTCCACCTGTCC
  CCCCGAGGACCACCCACATCTGAGCCCCCACCTGTGCCAGCCAACAAGCCCAAGTTCCTG
  AAGATAGCTGAAGAGGACCCCCCAAGGGAGGCAGCCATGCCCGGACTCTTTGTGCCCCCC
  GTGGCTCCCCGGCCTCCTGCGCTGAAGCTGCCAGTGCCTGAGGCCATGGCGCGGCCCGCA
  GTCCTGCCCAGGCCAGAGAAGCCGCAGCTCCCGCACCTCCAGCGATCACCCCCCGATGGG
  CAGAGTTTCAGGAGCTTCTCCTTTGAAAAGCCCCGGCAACCCTCACAGGCTGACACTGGC
  GGGGACGACTCGGACGAGGACTATGAGAAGGTGCCACTGCCCAACTCGGTCTTCGTCAAC
  ACCACGGAGTCCTGCGAAGTGGAAAGGTTGTTCAAGGCTACAAGCCCCCGGGGAGAGCCC
  CAGGATGGACTCTACTGCATCCGGAACTCCTCTACCAAGTCGGGGAAGGTCCTGGTTGTG
  TGGGACGAAACCTCTAACAAAGTGAGGAACTATCGCATTTTTGAGAAGGACTCTAAGTTC
  TACCTGGAGGGCGAGGTCCTGTTTGTGAGTGTGGGCAGCATGGTGGAGCACTACCACACC
  CACGTGCTGCCCAGCCACCAGAGCCTGCTGCTGCGGCACCCCTACGGCTACACTGGGCCT
  AGGTGA
  
  Clone variation with respect to NM_003023.4
  300 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003023 unedited
  GGGAGTCGGATTTGTATACGACTCATATAGGCGGCCGCGNATTCAGATCTGGTACCGGTC
  CGGAATTCCCGGGATATCGTCGACCCACGCGTCCGGCGGGCGGGACGGAGCTGCGGCGGC
  GGCGGGTCCCGCGGGCGGGAGCGCCTGGCGGCCGAGTCAAGCCGCCGCCTCGACCCAGGG
  CCCGCGGGCCAGGAGAGCGCTCGGCGGCTTCATGGCGGCTGAAGAGATGCATTGGCCTGT
  CCCTATGAAGGCCATTGGTGCCCAGAACCTGCTAACCATGCCTGGGGGCGTGGCCAAGGC
  TGGCTACCTGCACAAGAAGGGCGGTACCCAGCTGCAGCTGCTGAAATGGCCCCTGCGCTT
  TGTCATCATCCACAAACGCTGCGTCTACTACTTCAAGAGTAGCACCTCTGCCTCCCCGCA
  GGGCGCCTTCTCCCTGAGTGGCTATAACCGGGTGATGCGGGCGGCTGAGGAGACCACGTC
  CAACAACGTTTTCCCCTTCAAGATCATCCACATCAGCAAGAAGCACCGCACGTGGTTCTT
  CTCGGCCTCCTCCGAGGAGGAGCGCAAGAGCTGGATGGCCTTGCTGCGCAGGGAGATTGG
  CCACTTCCACGAAAAGAAAGACCTGCCCTTGGACACCAGCGACTCCAGCTCGGACACAGA
  CAGCTTCTACGGCGCAGTTGAGCGGCCTGTGGATATCAGCCTTTTCCCGTACCCCACGGN
  ACATGAAGACTATGAGCACGACGATGAGGATGACTCCTACCTGGAGCCTGACTCCCCGGA
  GCCCGGAAGGGCTGAGATGCCCTGATGCACCCACCGGCTTACCCACCACCCCCAGTGCCC
  ACGCCCAGGAAGCCAGCCTTCTCTGACATGGCCCGGGCCCACTCCTTTACCTC
  
• Restriction Sites: Please inquire
• ACCN: NM_003023
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003023.2, NP_003014.2
• RefSeq Size: 7300 bp
• RefSeq ORF: 1686 bp
• Locus ID: 6452
• Cytogenetics: 4p16.3
• Domains: SH2, PH
• Protein Families: Druggable Genome
• Protein Pathways: Natural killer cell mediated cytotoxicity
• Gene Summary: 'The protein encoded by this gene has an N-terminal pleckstrin homology (PH) domain, an SH3-binding proline-rich region, and a C-terminal SH2 domain. The protein binds to the SH3 domains of several proteins including the ABL1 and SYK protein tyrosine kinases , and functions as a cytoplasmic adaptor protein to positively regulate transcriptional activity in T, natural killer (NK), and basophilic cells. Mutations in this gene result in cherubism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]'
  Transcript Variant: This variant (1) represents use of an alternative promoter and 5' UTR and uses a downstream translation start site, compared to variant 3. The resulting isoform (a) has a shorter N-terminus, compared to isoform b. Both variants 1 and 2 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.