Keratin 12 (KRT12) (NM_000223) Human Untagged Clone

CAT#: SC300027

KRT12 (untagged)-Human keratin 12 (KRT12)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KRT12
• Synonyms: K12; MECD1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000223, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGATCTCTCCAACAACACCATGTCACTCTCAGTGCGCACCCCCGGACTGTCCCGGCGGCTCTCCTCGC
  AGAGTGTGATAGGCAGACCCAGGGGCATGTCTGCTTCCAGTGTTGGAAGTGGTTATGGGGGAAGTGCCTT
  TGGCTTTGGAGCCAGCTGTGGGGGAGGCTTTTCTGCTGCTTCCATGTTTGGTTCTAGTTCCGGCTTTGGG
  GGTGGCTCCGGAAGTTCCATGGCAGGAGGACTGGGTGCTGGTTATGGGAGAGCCCTGGGTGGAGGTAGCT
  TTGGAGGGCTGGGGATGGGATTTGGGGGCAGCCCAGGAGGTGGCTCTCTAGGTATTCTCTCGGGCAATGA
  TGGAGGCCTTCTTTCTGGATCAGAAAAAGAAACTATGCAAAATCTTAATGATAGATTAGCTTCCTACCTG
  GATAAGGTGCGAGCTCTAGAAGAGGCTAATACTGAGCTAGAAAATAAAATTCGAGAATGGTATGAAACAC
  GAGGAACTGGGACTGCAGATGCTTCACAGAGCGATTACAGCAAATATTATCCACTGATTGAAGACCTCAG
  GAATAAGATCATTTCAGCCAGCATTGGAAATGCCCAGCTCCTCTTGCAGATTGACAATGCGAGACTAGCT
  GCTGAGGACTTCAGGATGAAGTATGAGAATGAACTGGCCCTGCGCCAGGGCGTAGAGGCCGACATCAATG
  GCCTGCGCCGGGTGCTGGACGAGCTGACCCTGACCAGGACCGACCTGGAGATGCAGATCGAGAGCCTGAA
  CGAGGAGCTGGCCTACATGAAGAAGAACCACGAGGATGAGCTCCAAAGCTTCCGGGTGGGCGGCCCAGGC
  GAGGTCAGCGTAGAAATGGACGCTGCCCCCGGAGTGGACCTCACCAGGCTCCTCAATGATATGCGGGCGC
  AGTATGAAACCATCGCTGAGCAGAATCGGAAGGACGCTGAAGCCTGGTTCATTGAAAAGAGCGGGGAGCT
  CCGTAAGGAGATTAGCACCAACACCGAGCAGCTTCAGTCCAGCAAGAGCGAGGTCACCGACCTGCGTCGC
  GCCTTTCAGAACCTGGAGATCGAGCTACAGTCCCAGCTCGCCATGAAGAAATCCCTGGAGGACTCCTTGG
  CCGAAGCCGAGGGCGATTACTGCGCGCAGCTGTCCCAGGTGCAGCAGCTCATCAGCAACCTGGAGGCACA
  GCTGCTCCAGGTGCGCGCGGACGCAGAGCGCCAGAACGTGGACCACCAGCGGCTGCTGAATGTCAAGGCC
  CGCCTGGAGCTGGAGATTGAGACCTACCGCCGCCTGCTGGACGGGGAGGCCCAAGGTGATGGTTTGGAGG
  AAAGTTTATTTGTGACAGACTCCAAATCACAAGCACAGTCAACTGATTCCTCTAAAGACCCAACCAAAAC
  CCGAAAAATCAAGACAGTTGTGCAGGAGATGGTGAATGGTGAGGTGGTCTCATCTCAAGTTCAGGAAATT
  GAAGAACTAATGTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_000223
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000223.3, NP_000214.1
• RefSeq Size: 1992 bp
• RefSeq ORF: 1485 bp
• Locus ID: 3859
• Cytogenetics: 17q21.2
• Gene Summary: 'KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy. [provided by RefSeq, Jul 2008]'