Collagen X (COL10A1) (NM_000493) Human Untagged Clone

CAT#: SC300081

COL10A1 (untagged)-Human collagen, type X, alpha 1 (COL10A1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: COL10A1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000493 edited
  CCAGCACGCAGAATCCATCTGAGAATATGCTGCCACAAATACCCTTTTTGCTGCTAGTAT
  CCTTGAACTTGGTTCATGGAGTGTTTTACGCTGAACGATACCAAATGCCCACAGGCATAA
  AAGGCCCACTACCCAACACCAAGACACAGTTCTTCATTCCCTACACCATAAAGAGTAAAG
  GTATAGCAGTAAGAGGAGAGCAAGGTACTCCTGGTCCACCAGGCCCTGCTGGACCTCGAG
  GGCACCCAGGTCCTTCTGGACCACCAGGAAAACCAGGCTACGGAAGTCCTGGACTCCAAG
  GAGAGCCAGGGTTGCCAGGACCACCGGGACCATCAGCTGTAGGGAAACCAGGTGTGCCAG
  GACTCCCAGGAAAACCAGGAGAGAGAGGACCATATGGACCAAAAGGAGATGTTGGACCAG
  CTGGCCTACCAGGACCCCGGGGCCCACCAGGACCACCTGGAATCCCTGGACCGGCTGGAA
  TTTCTGTGCCAGGAAAACCTGGACAACAGGGACCCACAGGAGCCCCAGGACCCAGGGGCT
  TTCCTGGAGAAAAGGGTGCACCAGGAGTCCCTGGTATGAATGGACAGAAAGGGGAAATGG
  GATATGGTGCTCCTGGTCGTCCAGGTGAGAGGGGTCTTCCAGGCCCTCAGGGTCCCACAG
  GACCATCTGGCCCTCCTGGAGTGGGAAAAAGAGGTGAAAATGGGGTTCCAGGACAGCCAG
  GCATCAAAGGTGATAGAGGTTTTCCGGGAGAAATGGGACCAATTGGCCCACCAGGTCCCC
  AAGGCCCTCCTGGGGAACGAGGGCCAGAAGGCATTGGAAAGCCAGGAGCTGCTGGAGCCC
  CAGGCCAGCCAGGGATTCCAGGAACAAAAGGTCTCCCTGGGGCTCCAGGAATAGCTGGGC
  CCCCAGGGCCTCCTGGCTTTGGGAAACCAGGCTTGCCAGGCCTGAAGGGAGAAAGAGGAC
  CTGCTGGCCTTCCTGGGGGTCCAGGTGCCAAAGGGGAACAAGGGCCAGCAGGTCTTCCTG
  GGAAGCCAGGTCTGACTGGACCCCCTGGGAATATGGGACCCCAAGGACCAAAAGGCATCC
  CGGGTAGCCATGGTCTCCCAGGCCCTAAAGGTGAGACAGGGCCAGCTGGGCCTGCAGGAT
  ACCCTGGGGCTAAGGGTGAAAGGGGTTCCCCTGGGTCAGATGGAAAACCAGGGTACCCAG
  GAAAACCAGGTCTCGATGGTCCTAAGGGTAACCCAGGGTTACCAGGTCCAAAAGGTGATC
  CTGGAGTTGGAGGACCTCCTGGTCTCCCAGGCCCTGTGGGCCCAGCAGGAGCAAAGGGAA
  TGCCCGGACACAATGGAGAGGCTGGCCCAAGAGGTGCCCCTGGAATACCAGGTACTAGAG
  GCCCTATTGGGCCACCAGGCATTCCAGGATTCCCTGGGTCTAAAGGGGATCCAGGAAGTC
  CCGGTCCTCCTGGCCCAGCTGGCATAGCAACTAAGGGCCTCAATGGACCCACCGGGCCAC
  CAGGGCCTCCAGGTCCAAGAGGCCACTCTGGAGAGCCTGGTCTTCCAGGGCCCCCTGGGC
  CTCCAGGCCCACCAGGTCAAGCAGTCATGCCTGAGGGTTTTATAAAGGCAGGCCAAAGGC
  CCAGTCTTTCTGGGACCCCTCTTGTTAGTGCCAACCAGGGGGTAACAGGAATGCCTGTGT
  CTGCTTTTACTGTTATTCTCTCCAAAGCTTACCCAGCAATAGGAACTCCCATACCATTTG
  ATAAAATTTTGTATAACAGGCAACAGCATTATGACCCAAGGACTGGAATCTTTACTTGTC
  AGATACCAGGAATATACTATTTTTCATACCACGTGCATGTGAAAGGGACTCATGTTTGGG
  TAGGCCTGTATAAGAATGGCACCCCTGTAATGTACACCTATGATGAATACACCAAAGGCT
  ACCTGGATCAGGCTTCAGGGAGTGCCATCATCGATCTCACAGAAAATGACCAGGTGTGGC
  TCCAGCTTCCCAATGCCGAGTCAAATGGCCTATACTCCTCTGAGTATGTCCACTCCTCTT
  TCTCAGGATTCCTAGTGGCTCCAATGTGAGTACACCCCACAGAGCTAATCTAAATCTTGT
  GCTAGAAAAAGCATTCTCTAACTCTACCCCACCCTACAAAATGCATATGGAGGTAGGCTG
  AAAAGAATGTAATTTTTATTTTCTGAAATACAGATTTGAGCTATCAGACCAACAAACCTT
  CCCCCTGAAAAGTGAGCAGCA
  
• Restriction Sites: Please inquire
• ACCN: NM_000493
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000493.2, NP_000484.2
• RefSeq Size: 3285 bp
• RefSeq ORF: 2043 bp
• Locus ID: 1300
• Cytogenetics: 6q22.1
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes the alpha chain of type X collagen, a short chain collagen expressed by hypertrophic chondrocytes during endochondral ossification. Unlike type VIII collagen, the other short chain collagen, type X collagen is a homotrimer. Mutations in this gene are associated with Schmid type metaphyseal chondrodysplasia (SMCD) and Japanese type spondylometaphyseal dysplasia (SMD). [provided by RefSeq, Jul 2008]'