ARHGAP28 (NM_001010000) Human Untagged Clone

CAT#: SC301472

ARHGAP28 (untagged)-Human Rho GTPase activating protein 28 (ARHGAP28)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARHGAP28
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001010000, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGGAAAAAGGATAAGCAATCTATCAGGGATGTCAGAGACATTTTTGGAGTCAGTGAATCTCCTCCTC
  GTGATACCTGTGGCAACCACACTAATCAGCTGGATGGCACCAAGGAAGAAAGAGAGCTTCCAAGAGTTAT
  CAAGACAAGTGGTTCCATGCCAGATGATGCTTCTCTCAACAGTACTACCCTGTCTGACGCATCCCAGGAT
  AAAGAAGGGAGTTTTGCGGTTCCCAGGAGTGACTCTGTGGCTATACTTGAGACCATTCCAGTTCTACCAG
  TTCATTCCAATGGATCACCGGAGCCTGGACAGCCAGTTCAGAATGCGATAAGTGATGATGATTTTCTGGA
  AAAGAACATTCCACCAGAGGCTGAAGAGCTGTCATTTGAAGTGTCTTATTCAGAAATGGTTACGGAGGCT
  CTAAAAAGAAATAAACTTAAGAAATCAGAGATTAAGAAAGAAGACTATGTTTTAACTAAATTTAATGTTC
  AGAAAACCAGATTTGGCTTAACTGAAGCAGGAGATCTGTCTGCTGAAGACATGAAGAAAATCCGCCATCT
  CTCTCTGATTGAATTGACTGCCTTTTTTGATGCCTTTGGAATTCAACTGAAAAGGAACAAAACAGAGAAA
  GTAAAAGGACGAGACAATGGGATTTTTGGAGTTCCACTTACAGTCCTCCTGGACGGTGACCGAAAGAAAG
  ACCCTGGAGTGAAAGTTCCCCTGGTATTACAAAAATTTTTTGAGAAAGTTGAGGAATCAGGTCTGGAATC
  TGAAGGAATTTTTCGACTTTCAGGATGTACTGCTAAAGTCAAGCAATACCGTGAAGAACTTGATGCCAAG
  TTTAATGCTGATAAATTTAAATGGGACAAAATGTGCCATAGAGAAGCTGCAGTAATGTTGAAAGCGTTTT
  TCAGAGAACTACCCACCTCTCTCTTCCCTGTGGAATATATACCTGCCTTCATCAGTCTAATGGAAAGAGG
  GCCTCACGTCAAAGTACAGTTTCAAGCCTTACACCTCATGGTCATGGCGCTGCCTGATGCCAACAGAGAT
  GCAGCTCAGGCCCTCATGACATTCTTCAATAAAGTGATTGCCAATGAATCAAAAAACCGAATGAGTCTGT
  GGAACATTTCTACAGTGATGGCACCAAACCTTTTCTTCAGTAGAAGCAAACACTCTGATTATGAAGAATT
  ACTGTTAGCAAACACTGCGGCCCACATCATCCGCCTAATGCTTAAGTACCAGAAGATTTTGTGGAAGGTT
  CCATCTTTCTTAATCACTCAAGTAAGAAGAATGAATGAAGCCACGATGCTATTGAAGAAGCAGCTCCCAA
  GTGTCAGGAAGCTGCTCAGGAGGAAGACCCTCGAGCGGGAGACTGCAAGCCCCAAGACTTCAAAGGTACT
  GCAAAAATCACCCTCGGCAAGACGAATGTCTGACGTGCCGGAAGGAGTCATACGGGTCCATGCTCCACTT
  CTCTCCAAGGTGTCCATGGCCATTCAACTCAACAATCAAACCAAAGCCAAAGACATATTGGCAAAATTTC
  AATATGAAAACAGTCATGGTTCATCAGAATGTATTAAGATTCAGAACCAAAGGTTATATGAAATTGGAGG
  AAATATAGGAGAGCATTGCTTGGATCCAGATGCTTATATATTGGATGTATATCGTATAAATCCTCAAGCA
  GAATGGGTGATTAAACCCCAACAAAGTTCTTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001010000
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001010000.2, NP_001010000.1
• RefSeq Size: 5542 bp
• RefSeq ORF: 1713 bp
• Locus ID: 79822
• Cytogenetics: 18p11.31