BNC1 (NM_001717) Human Untagged Clone

CAT#: SC303057

BNC1 (untagged)-Human basonuclin 1 (BNC1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BNC1
• Synonyms: BNC; BSN1; HsT19447
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001717, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCGGCGGCGCCCGCCGAGCCGGGGCGGACGCGGGGCGGCCCGGGCCCGGGAGACGCGCCGGCAGCCCC
  GGCACCGCAGCGGTCGCAGGATGGCCGAGGCTATCAGCTGTACTCTGAACTGTAGTTGCCAAAGTTTCAA
  ACCCGGGAAAATAAACCACCGTCAGTGTGACCAATGCAAGCATGGATGGGTGGCCCACGCTCTAAGTAAG
  CTAAGGATCCCCCCCATGTATCCAACAAGCCAGGTGGAGATTGTCCAGTCCAATGTAGTGTTTGATATTA
  GCAGCCTCATGCTCTATGGGACCCAGGCCATCCCCGTTCGCCTAAAAATCCTACTGGACCGGCTCTTCAG
  TGTGTTGAAGCAAGATGAGGTTCTCCAGATCCTCCATGCCTTGGACTGGACACTTCAGGATTATATCCGT
  GGATACGTACTGCAGGATGCATCAGGAAAGGTGTTGGATCACTGGAGCATCATGACCAGTGAGGAAGAAG
  TGGCCACCTTGCAGCAGTTCCTTCGTTTTGGAGAGACCAAATCTATAGTTGAACTCATGGCAATTCAAGA
  GAAAGAAGAGCAATCCATCATCATACCACCTTCCACAGCAAATGTAGATATCAGGGCTTTCATCGAGAGC
  TGCAGTCACAGGAGTTCTAGCCTCCCCACTCCTGTGGACAAAGGAAACCCCAGCAGTATACACCCCTTTG
  AGAACCTCATAAGCAACATGACTTTCATGCTGCCTTTCCAGTTCTTCAACCCTCTGCCTCCTGCACTGAT
  AGGGTCATTGCCCGAACAATATATGTTGGAGCAGGGTCATGACCAAAGTCAGGACCCCAAACAGGAAGTC
  CATGGGCCCTTCCCTGACAGCAGCTTCTTAACTTCCAGTTCCACACCATTTCAGGTTGAAAAAGATCAGT
  GTTTAAACTGTCCGGATGCTATTACTAAAAAAGAAGACAGCACCCATTTAAGTGACTCCAGCTCATACAA
  CATTGTCACTAAGTTTGAAAGGACACAGTTATCCCCTGAGGCCAAAGTGAAGCCTGAGAGGAATAGCCTT
  GGTACAAAGAAGGGCCGGGTGTTCTGCACTGCATGTGAGAAGACCTTCTATGACAAAGGCACCCTCAAAA
  TCCACTACAATGCCGTCCACTTGAAGATCAAGCATAAGTGCACCATCGAAGGGTGTAACATGGTGTTCAG
  CTCCCTAAGGAGCCGGAATCGCCATAGCGCCAACCCCAACCCTCGGCTGCACATGCCAATGAACAGAAAT
  AACCGGGACAAAGACCTCAGGAACAGCCTGAACCTGGCCAGCTCTGAGAACTACAAGTGCCCAGGTTTCA
  CAGTGACGTCCCCAGACTGTAGGCCTCCTCCCAGCTACCCTGGTTCAGGAGAGGATTCCAAAGGCCAACC
  AGCCTTCCCAAACATTGGGCAAAATGGTGTGCTTTTTCCCAACCTAAAGACAGTCCAGCCAGTCCTTCCT
  TTCTACCGCAGTCCAGCCACGCCTGCCGAGGTAGCAAACACGCCTGGGATACTCCCTTCCCTCCCGCTGT
  TGTCCTCTTCAATCCCAGAACAGCTCATTTCAAACGAAATGCCATTTGATGCCCTTCCCAAGAAGAAATC
  CAGGAAGTCCAGTATGCCTATCAAAATAGAGAAAGAAGCTGTGGAAATAGCTAATGAGAAAAGACACAAC
  CTCAGCTCAGATGAAGACATGCCCCTACAGGTGGTCAGTGAAGATGAGCAGGAGGCCTGCAGTCCTCAGT
  CACACAGAGTATCTGAGGAGCAGCATGTACAGTCAGGAGGCTTAGGGAAGCCTTTCCCTGAAGGGGAGAG
  GCCCTGCCATCGTGAATCAGTAATTGAGTCCAGTGGAGCCATCAGCCAAACCCCTGAGCAGGCCACACAC
  AATTCAGAGAGGGAGACTGAGCAGACACCAGCATTGATCATGGTGCCAAGGGAGGTCGAGGATGGTGGCC
  ATGAACACTACTTCACACCTGGGATGGAACCCCAAGTTCCTTTTTCTGACTACATGGAACTGCAGCAGCG
  CCTGCTGGCTGGGGGACTCTTCAGTGCTTTGTCCAACAGGGGAATGGCTTTTCCTTGTCTTGAAGATTCT
  AAAGAACTGGAGCACGTGGGTCAGCATGCATTAGCAAGGCAGATAGAAGAAAATCGCTTCCAGTGTGACA
  TCTGCAAGAAGACCTTTAAAAATGCTTGTAGTGTGAAAATTCATCACAAGAATATGCATGTCAAAGAAAT
  GCACACATGCACAGTGGAGGGCTGTAATGCTACCTTTCCCTCCCGCAGGAGCAGAGACAGACACAGCTCA
  AACCTAAACCTCCACCAAAAAGCATTGAGCCAGGAAGCATTGGAGAGTAGTGAAGATCATTTCCGTGCAG
  CTTACCTTCTGAAAGATGTGGCTAAGGAAGCCTATCAGGATGTGGCTTTTACACAGCAAGCCTCCCAGAC
  ATCTGTCATCTTCAAAGGAACAAGTCGAATGGGCAGTCTGGTTTACCCAATAACGCAAGTCCACAGTGCC
  AGCCTGGAGAGCTACAACTCTGGCCCCTTGAGCGAGGGCACCATCCTGGATTTGAGCACTACCTCGAGCA
  TGAAGTCAGAGAGTAGCAGCCATTCTTCCTGGGACTCTGACGGGGTGAGTGAGGAAGGCACTGTGCTTAT
  GGAGGACAGTGATGGGAACTGTGAAGGGTCGAGCCTTGTCCCTGGGGAAGATGAGTACCCCATCTGTGTC
  CTGATGGAGAAGGCTGACCAGAGCCTTGCTAGCCTGCCTTCTGGGTTGCCCATAACCTGTCATCTCTGCC
  AAAAGACATACAGTAACAAAGGGACCTTTAGGGCCCACTACAAAACTGTGCACCTCCGGCAGCTCCACAA
  ATGCAAAGTACCAGGCTGCAACACCATGTTTTCGTCTGTTCGCAGTCGAAACAGACACAGCCAGAATCCC
  AACCTGCACAAAAGCCTGGCCTCATCTCCAAGTCACCTCCAGTAA
  
  
• Restriction Sites: Please inquire
• ACCN: NM_001717
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001717.2, NP_001708.2
• RefSeq Size: 4612 bp
• RefSeq ORF: 2985 bp
• Locus ID: 646
• Cytogenetics: 15q25.2
• Gene Summary: 'This gene encodes a zinc finger protein present in the basal cell layer of the epidermis and in hair follicles. It is also found in abundance in the germ cells of testis and ovary. This protein is thought to play a regulatory role in keratinocyte proliferation and it may also be a regulator for rRNA transcription. Disruption of this gene has been implicated in premature ovarian failure as well as testicular premature aging. [provided by RefSeq, Sep 2020]'
  Transcript Variant: This variant (1) encodes the longer isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.